| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
|
|---|
| Outline of Final Research Achievements |
A zinc finger protein, GATA4, associates with an intrinsic histone acetyltransferase (HAT), p300, and regulates myocardial transcriptional activities in response to hypertrophic stimuli. In this study, we demonstrated whether GATA4 formed a homo-dimer and its functional mechanism. Chemical cross-linking assay using nuclear extract from HEK293T cells showed that GATA4 homo-dimer was increased by co-expression of p300. IP-WB assay demonstrated that co-expression of GATA4 with p300 increased the formation of its homo-dimer as well as its acetylation. HAT-deficient mutant of p300 and acetyl-deficient mutant of GATA4 did not increase this homo-dimerization as well as GATA4-acetylation. These results suggest that acetylation of GATA4 is required for the formation of GATA4 dimerization. Thus, this dimerization may regulate hypertrophy-response gene activations and the development of heart failure.
|
