Bystander effect induction mechanisms for radiotherapy optimizing biological effect

• Project/Area Number: 15K21313
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Medical Physics and Radiological Technology; Radiation science
• Research Institution: Hokkaido University of Science
• Principal Investigator: Sasaki Kohei 北海道科学大学, 保健医療学部, 准教授 (20736376)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: バイスタンダー効果 / モンテカルロ法 / 放射線影響 / コンピュータシミュレーション / DNA二本鎖切断 / 非標的効果 / シグナル伝達 / 活性酸素種 / カルシウムイオン / 数理モデル / モンテカルロシミュレーション

Outline of Final Research Achievements

This research aims to develop a method to estimate radiation effects using computer simulation. By conducting computer simulation using the phenomenon as a mathematical model, it becomes possible to cross-analyze knowledge obtained by cell biology and molecular biology.
New findings obtained in this research can be applied to analysis of effects on living cells due to radiation exposure and prediction of radiotherapy effect.

Research Products

• [Journal Article] Integrated Modelling of Cell Responses after Irradiation for DNA-Targeted Effects and Non-Targeted Effects (2018)
  • Author(s): Yusuke Matsuya, Kohei Sasaki, Yuji Yoshii, Go Okuyama and Hiroyuki Date
  • Journal Title: Scientific Reports Volume: 8 Issue: 1 Pages: 1-14
  • DOI: 10.1038/s41598-018-23202-y
  • NAID: 120006463569
  • Peer Reviewed / Open Access
• [Journal Article] Modeling cell survival and change in amount of DNA during protracted irradiation (2016)
  • Author(s): Y. Matsuya, K. Tsutsumi, K. Sasaki, Y. Yoshii, T. Kimura, H. Date
  • Journal Title: Journal of Radiation Research Volume: - Issue: 3 Pages: 1-11
  • DOI: 10.1093/jrr/rrw110
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Investigation of cell survival under protracted exposure for various dose rates in consideration of cell cycle distributionInvestigation of cell survival under protracted exposure for various dose rates in consideration of cell cycle distribution (2017)
  • Author(s): Yusuke Matsuya, Stephen J. McMahon, Kaori Tsutsumi, Kohei Sasaki, Yuji Yoshii, Ryosuke Mori, Joma Oikawa, Hiroyuki Date, Kevin M. Prise
  • Organizer: 第60回大会日本放射線影響学会
• [Presentation] A simulation study for both of the targeted and untargeted effect on the uniformly irradiated cells (2017)
  • Author(s): Kohei Sasaki
  • Organizer: ERRS and GBS 2017
  • Int'l Joint Research / Invited
• [Presentation] 生物学的影響を考慮した新たなCT被ばく線量評価指標の検討 (2017)
  • Author(s): 谷口 広夢，小林 穂乃香，関口 綾乃，成田 明里咲，真田 哲也，渋谷 幸喜，佐々木 恒平
  • Organizer: 日本放射線技術学会北海道支部第73回秋季大会
• [Presentation] DNA損傷シミュレーションによる臓器線量評価法の検討 (2017)
  • Author(s): 小林 穂乃香，谷口 広夢，関口 綾乃，成田 明里咲，真田 哲也，渋谷 幸喜，佐々木 恒平
  • Organizer: 日本放射線技術学会北海道支部第73回秋季大会
• [Presentation] 低酸素細胞の放射線抵抗性の解明に向けたモンテカルロシミュレーション（I. 直接効果） (2017)
  • Author(s): 関口 綾乃，成田 明里咲，小林 穂乃香，谷口 広夢，真田 哲也 ，八重樫 祐司 ，佐々木 恒平
  • Organizer: 日本放射線技術学会北海道支部第73回秋季大会
• [Presentation] 低酸素細胞の放射線抵抗性の解明に向けたモンテカルロシミュレーション（II. 間接効果） (2017)
  • Author(s): 成田 明里咲，関口 綾乃，谷口 広夢，小林 穂乃香，真田 哲也 ，八重樫 祐司 ，佐々木 恒平
  • Organizer: 日本放射線技術学会北海道支部第73回秋季大会
• [Presentation] Biophysical modeling for targeted and non-targeted effects on cells after irradiation (2016)
  • Author(s): Matsuya Y, Sasaki K, Yoshii Y and Date H
  • Organizer: International Conference on Medical Physics 2016 (ICMP2016)
  • Place of Presentation: バンコク、タイ
  • Year and Date: 2016-12-09
  • Int'l Joint Research
• [Presentation] Estimation of DNA damage kinetics in both targeted and nontargeted cells using a stochastic model with the microdosimetric analyses (2016)
  • Author(s): K. Sasaki, Y. Matsuya and H. Date
  • Organizer: 2016 Annual meeting of Radiation Research Society
  • Place of Presentation: ハワイ島、米国
  • Year and Date: 2016-10-16
  • Int'l Joint Research
• [Presentation] CELL-KILLING MODEL IN CONSIDERATION OF DNA DAMAGE REPAIR AND CELL PHASE (2016)
  • Author(s): Y. Matsuya, Y. Yoshii, K. Sasaki and H. Date
  • Organizer: ESRAH2016
  • Place of Presentation: 札幌、日本
  • Year and Date: 2016-10-01
  • Int'l Joint Research
• [Presentation] THE EFFECT OF SECONDARY ELECTRON ON DNA DOUBLE-STRAND BREAKS AFTER PHOTON IRRADIATION (2016)
  • Author(s): Y. Yoshii, Y. Matsuya, K. Sasaki and H. Date
  • Organizer: ESRAH2016
  • Place of Presentation: 札幌、日本
  • Year and Date: 2016-10-01
  • Int'l Joint Research
• [Presentation] Stochastic modeling of intercellular signaling for estimating DNA damage with confluent cell monolayers (2015)
  • Author(s): Kohei Sasaki
  • Organizer: 15th International Congress of Radiation Research (ICRR2015) - Symposium " Re-evaluation of Biological Targets of Radiation-induced Cell Killing"
  • Place of Presentation: Kyoto International Conference Center
  • Year and Date: 2015-05-25
  • Int'l Joint Research / Invited
• [Remarks]
  • URL: http://labs.hus.ac.jp/