The study of exocytosis associated with regulation of lipid raft via MARCKS phosphorylation

• Project/Area Number: 15K21322
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General physiology; Functional basic dentistry
• Research Institution: Asahi University (2016-2017); Dokkyo Medical University (2015)
• Principal Investigator: Satoh Keitaro 朝日大学, 歯学部, 准教授 (10549041)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 開口分泌 / アミラーゼ / MARCKS / 耳下腺 / 膵外分泌腺 / 脂質ラフト / 開口放出 / アミラーゼ分泌

Outline of Final Research Achievements

I investigated the involvement of myristoylated alanine-rich C kinase substrate (MARCKS) phosphorylation in amylase release in parotid and pancreatic acinar cells. Concretely, I examined as the study which are associated with regulation of lipid raft on cell membrane. Findings from this study suggest that MARCKS, which is regulated by many signal transduction in lipid raft, play an important role for exocytosis.

Research Products

• [Journal Article] Involvement of myristoylated alanine-rich C kinase substrate phosphorylation and translocation in cholecystokinin-induced amylase release in pancreatic acini. (2016)
  • Author(s): Satoh K, Narita T, Katsumata-Kato O, Sugiya H, Seo Y
  • Journal Title: Am J Physiol Gastrointest Liver Physiol Volume: 310 Issue: 6 Pages: G399-G409
  • DOI: 10.1152/ajpgi.00198.2015
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 耳下腺および膵アミラーゼ分泌におけるMARCKSとPLDの関与 (2018)
  • Author(s): 佐藤 慶太郎，柏俣 正典
  • Organizer: 第95回日本生理学会大会
• [Presentation] ラット耳下腺腺房細胞βアゴニスト刺激によるアミラーゼ分泌におけるMARCKSリン酸化を介したPLDの関与 (2017)
  • Author(s): 佐藤 慶太郎, 柏俣 正典, 杉谷 博士
  • Organizer: 第94回日本生理学会
  • Place of Presentation: アクトシティ浜松
  • Year and Date: 2017-03-28
• [Presentation] Involvement of MARCKS phosphorylation and translocation in pancreatic amylase release (2017)
  • Author(s): Keitaro Satoh, Takanori Narita, Osamu Katsumata-Kato, Hiroshi Sugiya, Yoshiteru Seo
  • Organizer: Gordon Research Conferences Salivary Glands & Exocrine Secretion
  • Place of Presentation: Hotel Galvez
  • Year and Date: 2017-02-19
  • Int'l Joint Research
• [Presentation] 外分泌腺タンパク質分泌におけるMARCKSリン酸化と局在変化 (2017)
  • Author(s): 佐藤 慶太郎，柏俣 正典，瀬尾 芳輝，杉谷 博士
  • Organizer: 第59回歯科基礎医学会学術大会
• [Presentation] Involvement of MARCKS phosphorylation and translocation in amylase release in exocrine gland acinar cells (2016)
  • Author(s): Keitaro Satoh
  • Organizer: The 4th International Synposium on Salivary Glands in Honor of Niels Stensen
  • Place of Presentation: 岡崎コンファレンスセンター
  • Year and Date: 2016-11-30
  • Int'l Joint Research
• [Presentation] ラット膵外分泌腺腺房アミラーゼ分泌におけるMARCKSリン酸化および局在変化の関与 (2016)
  • Author(s): 佐藤 慶太郎, 成田 貴則, 加藤 治, 杉谷 博士, 瀬尾芳輝
  • Organizer: 第93回日本生理学会大会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2016-03-22
• [Presentation] 耳下腺腺房細胞におけるMARCKSリン酸化と局在変化のイソプロテレノール誘発性アミラーゼ分泌への関与 (2015)
  • Author(s): 佐藤 慶太郎, 成田 貴則, 加藤 治, 杉谷 博士
  • Organizer: 第57回歯科基礎医学会学術大会
  • Place of Presentation: 朱鷺メッセ
  • Year and Date: 2015-09-11