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Development and finding the mechanism of pancreatic beta cell differentiation from hiPS cells through Single cell analysis

Research Project

Project/Area Number 15K21333
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Life / Health / Medical informatics
Biomedical engineering/Biomaterial science and engineering
Research InstitutionNational Center for Global Health and Medicine (2016-2017)
Saitama Medical University (2015)

Principal Investigator

Sugahara Izumi  国立研究開発法人国立国際医療研究センター, その他部局等, 研究員 (10633000)

Research Collaborator Okazaki Yasushi  埼玉医科大学, 医学部, 客員教授 (80280733)
Matsumoto Masahito  埼玉医科大学, 医学部, 客員講師 (90321819)
Nakachi Yutaka  埼玉医科大学, 医学部, 助教 (10522097)
Watanabe Ami  東京大学, 定量生命科学研究所, 特任研究員 (40611421)
Nikaido Itoshi  国立研究開発法人理化学研究所, 生命機能科学研究センター, ユニットリーダー (00383290)
Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsbeta cell / single cell analysis / hiPS / single cell / β細胞 / hiPSダブルノックイン
Outline of Final Research Achievements

The human induced pluripotent stem cells are potential resource for type 1 diabetes cell based therapies. Although,the beta cell differentiation had been deeply investigated, the molecular mechanism of differentiation of beta cell from hiPSCs and in which levels were achieved the beta cell derived hiPSCs has not been understood completely. In this study we provide, a single cell transcriptome of differentiated beta cells from hiPSCs, in which double labelled with Venus and mCherry proteins (hIveNry). The Venus protein could express under INS promoter and mCherry under NGN3 promoter, meaning that by fluorescent protein could monitor from NGN3+ red endocrine progenitor to the Venus positive beta cells. In this study we performed the cell profiling of the fluorescent positive cells viewing the status of the differentiation of the beta cells in which the maturation process was confirmed by glucose responsivity.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (6 results)

All 2017 2016

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (5 results) (of which Int'l Joint Research: 3 results)

  • [Journal Article] An inhibitor of Fibroblast Growth Factor Receptor-1 (FGFR1) promotes late-stage terminal differentiation from NGN3+ pancreatic endocrine progenitors2016

    • Author(s)
      Yamashita-Sugahara Y, Matsumoto M, Ohtaka M, Nishimura K, Nakanishi M, Mitani K, Okazaki Y
    • Journal Title

      Scientific Reports

      Volume: 6 Issue: 1 Pages: 335908-335908

    • DOI

      10.1038/srep35908

    • NAID

      120007129280

    • Related Report
      2017 Annual Research Report 2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] FGFR1阻害剤は膵内分泌前駆細胞(NGN3+)から内分泌細胞への分化を促進する2017

    • Author(s)
      Yzumi Yamashita-Sugahara, Masahito Matsumoto,Manami Ohtaka,Ken Nishimura Mahito Nakanishi,Kohnosuke Mitani & Yasushi Okazaki
    • Organizer
      2016日本再生医療学会
    • Place of Presentation
      仙台
    • Year and Date
      2017-03-07
    • Related Report
      2016 Research-status Report
  • [Presentation] An inhibitor of fibroblast growth factor receptor-1 (FGFR1) promotes late-stage terminal differentiation from NGN3+ pancreatic endocrine progenitors2017

    • Author(s)
      Yzumi Yamashita-Sugahara, Masahito Matsumoto,Manami Ohtaka,Ken Nishimura Mahito Nakanishi,Kohnosuke Mitani & Yasushi Okazaki
    • Organizer
      2017 Miami winter Symposium: Diabetes
    • Place of Presentation
      Miami
    • Year and Date
      2017-01-22
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] An inhibitor of fibroblast growth factor receptor-1 (FGFR1) promotes late-stage terminal differentiation from NGN3+ pancreatic endocrine progenitors2017

    • Author(s)
      Yzumi Yamshita-Sugahara
    • Organizer
      Miami Winter Symposium 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] FGFR1 阻害剤は膵内分泌前駆細胞(NGN3+)から内分泌細胞への分化を促進する2017

    • Author(s)
      菅原 泉
    • Organizer
      第16回日本再生医療学会総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] FGFR1 阻害剤は膵内分泌前駆細胞(NGN3+)から内分泌細胞への分化を促進する2017

    • Author(s)
      菅原 泉
    • Organizer
      第14回RCGM国際シンポジウム 埼玉医科大学
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research

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Published: 2015-04-16   Modified: 2020-03-30  

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