Elucidation of mechanism to augment host innate immunity by Lactobacillus casei

• Project/Area Number: 15K21362
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Food science; Bacteriology (including mycology)
• Research Institution: Kitasato University
• Principal Investigator: ITO MASAHIRO 北里大学, 薬学部, 助教 (90596727)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: プロバイオティクス

Outline of Final Research Achievements

To clarify bacterial components associated with augmentation of host innate immunity, TNF-α expression was measured after infection of heat killed Lactobacillus casei ATCC 27139 to mouse spleen cell. The data indicates that the rhamnose, which is its surface component, is associated with augmentation of host innate immunity. Western blotting analysis of mouse spleen cell lysate with the rhamnose-biotin probe and mouse CD14 antibody suggests that CD14 serves as rhamnose binding C-type lectin. Taken together, it suggests that Lactobacillus casei ATCC 27139 augments host innate immunity by interacting its surface rhamnose with host CD14.