| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
Breast cancer is a heterogeneous disease classified into two biological subtypes. Basal-like subtype breast cancer, which is ER-, PR-, ERBB2- (TNBC) phenotype, shows higher malignancy than luminal type, and exhibits poor prognoses against various methods of therapy. In this study, we focused on the cancer stem cells (CSC), a malignant sub-population in the tumor. Using activated Ras and p53 knockout mammary epithelial cells, we found that a portion of cancer cells undergo epithelial to mesenchymal transition (EMT), one of mechanism of CSC generation. Therefore, we analyzed the mechanism of EMT and MET and demonstrate that the two populations significantly enhance the transition of cells from the other population to their own. We also demonstrate that primary breast cancer cells underwent EMT. Further studies to elucidate the molecular mechanisms governing the dynamic EMT and MET observed in breast cancer cells must be pursued to develop effective therapeutic strategies against TNBC.
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