Verification of the force-dependent binding of an alpha-catenin to an actn filament
| Project/Area Number |
15K21444
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biological physics/Chemical physics/Soft matter physics
Biophysics
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| Research Institution | Waseda University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 細胞間接着 / アクチンフィラメント / カテニン / 蛍光タンパク質 / タンパク質動態 / 多量体形成 / BiFCプローブ / αカテニン / 細胞接着 / 蛍光イメージング |
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| Outline of Final Research Achievements |
The application was planned to unravel the force dependency of binding property between alpha-catenin in cadherin-catenin complex (CCC) and actin filament (AF). However, just after the start of our research, other group published a report about identical interest. For that reason, we shifted main focus to the next interest of dynamic property of a-catenin dimerization in cells. To this end, we aimed developing novel genetically encoded fluorescence dimerization sensor by utilizing UnaG, because conventional dimerization sensors are unsuitable for analyzing dynamic association/dissociation of proteins. As essential information for the developing dimerization sensor from UnaG, we determined the monomeric dispersion of apoUnaG and holoUnaG and the association/dissociation rate constant of UnaG and bilirubin. Furthermore, we discover that holoUnaG take distinct two fluorescent states defined by 23% and 92% quantum efficiency, and fluctuate between the two states.
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Report
(3 results)
Research Products
(10 results)
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[Journal Article] In vivo cardiac nano-imaging: A new technology for high-precision analyses of sarcomere dynamics in the heart2017
Author(s)
Togo Shimozawa, Erisa Hirokawa, Fuyu Kobirumaki-Shimozawa, Kotaro Oyama, Seine A. Shintani, Takako Terui, Yasuharu Kushida, Seiichi Tsukamoto, Teruyuki Fujii, Shin'ichi Ishiwata, and Norio Fukuda
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Journal Title
Prog. Biophys. Mol. Biol.
Volume: 124
Pages: 31-40
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Nano-imaging of the beating mouse heart in vivo: Importance of sarcomere dynamics, as opposed to sarcomere length per se, in the regulation of cardiac function.2016
Author(s)
Kobirumaki-Shimozawa F, Oyama K, Shimozawa T, Mizuno A, Ohki T, Terui T, Minamisawa S, Ishiwata S, Fukuda N
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Journal Title
Journal of General Physiology
Volume: 147
Issue: 1
Pages: 53-62
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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