Improving cold stress tolerance of plants by spatiotemporal modulation of tapetal autophagy
Project/Area Number |
15K21450
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Crop production science
Plant nutrition/Soil science
|
Research Institution | Tokyo University of Technology |
Principal Investigator |
|
Project Period (FY) |
2015-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
Keywords | オートファジー / イネ / タペート細胞 / 花粉 / 温度ストレス / 転写制御 / プログラム細胞死 / ストレス耐性 |
Outline of Final Research Achievements |
We established an in vivo imaging system to analyze the dynamics of autophagic flux in rice tapetum cells by expressing a marker of autophagosomes (GFP-ATG8) under the control of multiple tapetum-specific promoters, and revealed the dynamics of autophagy during pollen maturation. We comprehensively analyzed the effect of autophagy disruption on phytohormone contents in rice anthers at the flowering stage, and found that endogenous levels of active-forms of gibberellins (GAs) were significantly lower in the autophagy-defective mutant than in the wild type, indicating that autophagy affects metabolism and endogenous levels of GAs in rice anthers. We also suggested the possible involvement of reactive oxygen species (ROS)-mediated signaling and the EAT1 transcriptional network in the regulation of autophagy and the proper timing of tapetal programmed cell death during anther development.
|
Report
(3 results)
Research Products
(77 results)
-
[Journal Article] Development of simple sequence repeat markers in the halophytic turf grass Sporobolus virginicus and transferable genotyping across multiple grass genera/species/genotypes2017
Author(s)
Endo, C., Yamamoto, N., Kobayashi, M., Nakamura, Y., Yokoyama, K., Kurusu, T., Tada, Y.
-
Journal Title
Euphytica
Volume: 213
Issue: 2
Pages: 1-12
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
[Journal Article] Autophagy supports biomass production and nitrogen use efficiency at the vegetative stage in rice2015
Author(s)
Wada S, Hayashida Y, Izumi M, Kurusu T, Hanamata S, Kanno K, Kojima S, Yamaya T, Kuchitsu K, Makino A, Ishida H
-
Journal Title
Plant Physiology
Volume: in press
Issue: 1
Pages: 60-73
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Roles of autophagy in rice seed development2016
Author(s)
Sera Y., Kurusu T., Hanamata S., Sakamoto S., Ono S., Kitahata N., Mitsui T., Nonomura K., Mitsuda N., Kuchitsu K.
Organizer
International Quantum Biofingormatics (QBIC) Workshop 2016
Place of Presentation
東京理科大学(千葉県野田市)
Year and Date
2016-10-12
Related Report
Int'l Joint Research
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Physiological roles of autophagy in the regulation of development and metabolism in rice2016
Author(s)
Kurusu T., Toh B., Sera Y., Sakamoto S., Hanamata S., Ono S., Okazaki Y., Nihira K., Kitahata N., Kojima M., Sakakibara H., Saito K., Nonomura K., Mitsuda N., Tada Y., Kuchitsu K.
Organizer
Annual Meeting of JSPP 2016
Place of Presentation
岩手大学上田キャンパス(岩手県盛岡市)
Year and Date
2016-03-18
Related Report
-
[Presentation] Physiological roles of autophagy in the regulation of development and metabolism in rice2016
Author(s)
Kurusu T., Toh B., Sera Y., Sakamoto S., Hanamata S., Ono S., Okazaki Y., Nihira K., Kitahata N., Kojima M., Sakakibara H., Saito K., Nonomura K., Mitsuda N., Tada Y., Kuchitsu K.
Organizer
TUS International symposium 2016
Place of Presentation
東京理科大学野田キャンパス(千葉県野田市)
Year and Date
2016-03-17
Related Report
Int'l Joint Research
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Roles of autophagy during male reproductive development and sexual reproduction in rice.2015
Author(s)
Kurusu T., Toh B., Hanamata S., Kubo T., Okazaki Y., Ohnishi T., Nagata N., Saito K., Kinoshita T., Kurata N., Tada Y., Kuchitsu K.
Organizer
(The 79th Annual Meeting of the Botanical Society of Japan
Place of Presentation
朱鷺メッセ(新潟県新潟市)
Year and Date
2015-09-07
Related Report
Int'l Joint Research / Invited
-
-
-
-
-
-
-
-
-
-
-
-