Chemical synthesis of oligonucleotides having keto sugar and their application to chemical biology study

Research Project

Project/Area Number	15K21451
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Chemical biology Biomolecular chemistry
Research Institution	Kanagawa University
Principal Investigator	Saneyoshi Hisao 神奈川大学, 工学部, 助教 (10564784)
Project Period (FY)	2015-04-01 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000) Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords	酸化損傷 / 還元反応 / 細胞内化学反応 / ケト基 / RNA / 結晶化 / ポスト反応 / 核酸合成 / 酸化的開裂
Outline of Final Research Achievements	This research aims to explore the unexplored nucleic acid functions and elucidate the biological functions through chemical synthesis of keto sugar type nucleic acid in which the sugar moiety is oxidized. Chemical synthesis of nucleic acids having a keto group at 2 'position was accomplished by incorporating a branched sugar nucleoside as a key precursor. The keto sugar-type nucleic acid showed a different thermal stability compared to DNA (deoxyribose) and RNA (ribose). The introduced keto group could be converted to a hydroxyl group by a reduction reaction in an aqueous solution.

Report

(4 results)