Basic analysis of antibacterial photodynamic therapy using periodontal pathogenic bacterium uptake pigment

• Project/Area Number: 15K21454
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Periodontology; Pathobiological dentistry/Dental radiology
• Research Institution: Kanagawa Dental College
• Principal Investigator: YOSHIDA AYAKA 神奈川歯科大学, 大学院歯学研究科, 特別研究員 (00609414)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 青色光 / Prophyromonas gingivalis / 抗菌光線力学療法 / Porphyromonas gingicalis / Porphyromonas gingivalis

Outline of Final Research Achievements

In recent years, the periodontitis treatment of antibacterial photodynamic therapy (aPDT) has attracted attention. The current method causes cytotoxicity by reactive oxygen species (ROS) induced by photoexcitation by dye staining. I investigated aPDT used for energy metabolism of periodontal pathogenic bacteria. It was examined that the effects of aPDT using pigment used for energy metabolism of periodontal pathogenic bacteria. It was recognized that bacterial peculiar pigment was excited and produced ROS by light irradiation. It caused bactericidal effect due to oxidation damage to DNA. According to this study, it was suggested that this aPDT has less influence on the living body than the method currently used and a possibility of a treatment method with high bactericidal effect against bacteria.

Research Products

• [Journal Article] Bactericidal effect of hydroxyl radicals generated by the sonolysis and photolysis of hydrogen peroxide for endodontic applications (2017)
  • Author(s): Ibi H, Hayashi M, Yoshino F, Tamura M, Yoshida A, Kobayashi Y, Shimizu K, Lee M-C, Imai K, Ogiso B
  • Journal Title: Microbial Pathogenesis Volume: 103 Pages: 65-70
  • DOI: 10.1016/j.micpath.2016.12.010
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Antimicrobial effect of titanium dioxide after ultraviolet irradiation against periodontal pathogen (2016)
  • Author(s): Shirai R, Miura T, Yoshida A, Yoshino F, Ito T, Yoshinari M, Yajima Y
  • Journal Title: Dental Materials Journal Volume: 35 Issue: 3 Pages: 511-516
  • DOI: 10.4012/dmj.2015-406
  • NAID: 130005155189
  • ISSN: 0287-4547, 1881-1361
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Protective effects of (6R)-5,6,7,8-tetrahydro-L-biopterin on local ischemia/reperfusion-induced suppression of reactive hyperemia in rat gingiva (2016)
  • Author(s): Tanaka Y, Toyama T, Wada-Takahashi S, Sasaki H, Miyamoto C, Maehata Y, Yoshino F, Yoshida A, Takahashi S-S, Watanabe K, Lee M-C, Todoki K, Hamada N
  • Journal Title: Journal of Clinical Biochemistry and Nutrition Volume: 58 Issue: 1 Pages: 69-75
  • DOI: 10.3164/jcbn.15-69
  • NAID: 130005116629
  • ISSN: 0912-0009, 1880-5086
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Porphyromonas gingivalis infection modifies oral microcirculation and aortic vascular function in the stroke-prone spontaneously hypertensive rat (SHRSP). (2016)
  • Author(s): Funaki S, Tokutomi F, Wada-Takahashi S, Yoshino F, Yoshida A, Maehata Y, Miyamoto C, Toyama T, Sato T, Hamada N, Lee M-C, Takahashi S-S
  • Journal Title: Microbial Pathogenesis Volume: 92 Pages: 36-42
  • DOI: 10.1016/j.micpath.2015.12.009
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] α-glucosyl hesperidin suppressed the exacerbation of 5-fluorouracil-induced oral mucositis in the hamster cheek pouch. (2016)
  • Author(s): Yoshino F, Yoshida A, Toyama T, Wada-Takahashi S, Takahashi S-S
  • Journal Title: Journal of Functional Foods Volume: 21 Pages: 223-231
  • DOI: 10.1016/j.jff.2015.12.008
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Blue light irradiation-induced oxidative stress in vivo via ROS generation in rat gingival tissue. (2015)
  • Author(s): Yoshida A, Shiotsu-Ogura Y, Wada-Takahashi S, Takahashi S-S, Toyama T, Yoshino F
  • Journal Title: Journal of Photochemistry and Photobiology B: Biology Volume: 151 Pages: 48-53
  • DOI: 10.1016/j.jphotobiol.2015.07.001
  • Peer Reviewed / Open Access
• [Journal Article] Relationship among salivary antioxidant activity, cytokines and periodontitis: the Nagasaki Island study. (2015)
  • Author(s): Tamaki N, Yoshino F, Fukui M, Hayashida H, Yoshida A, Kitamura M, Iwasaki T, Furugen R, Kawasaki K, Nakazato M, Maeda T, Kokeguchi S, Yamamoto T, Lee M-C, Ito H, Saito T
  • Journal Title: Journal of Clinical Periodontology Volume: 42 Issue: 8 Pages: 711-718
  • DOI: 10.1111/jcpe.12438
  • Peer Reviewed
• [Presentation] Antibacterial Activity of Infrared Free Electron Laser on Cariogenic Bacteria (2017)
  • Author(s): Toyama T, Fujioka J, Koyata Y, Sakuma T, Watanabe K, Sasaki H, Yoshino F, Yoshida A, Tsukiyama K, Hamada N
  • Organizer: 95th General session & Exhibition of the IADR, 46th Annual meeting of the AADR and 41th Annual meeting of the CADR
  • Place of Presentation: San Francisco, CA, USA
  • Year and Date: 2017-03-22
  • Int'l Joint Research
• [Presentation] 大気利用プラズマ発生装置を応用した新たな根管消毒法の基礎的検討 (2017)
  • Author(s): 平野頼是，林 誠，田村宗明，吉野文彦，吉田彩佳，小林慶美，井比陽奈，増渕光暁，小木曾文内
  • Organizer: 第18回日本口腔機能水学会学術大会
  • Place of Presentation: 千代田区
  • Year and Date: 2017-03-11
• [Presentation] 超音波分解と光分解によって過酸化水素から生成されるヒドロキシラジカルによる歯内療法条件下における殺菌効果 (2017)
  • Author(s): 井比陽奈，林 誠，吉野文彦，田村宗明，吉田彩佳，平野頼是，小林慶美， 今井健一，小木曾文内
  • Organizer: 第18回日本口腔機能水学会学術大会
  • Place of Presentation: 千代田区
  • Year and Date: 2017-03-11
• [Presentation] 青色発光ダイオード照射はラット口蓋歯肉の酸化ストレスを亢進する (2016)
  • Author(s): 吉田彩佳，小椋有香子，高橋聡子，高橋俊介，遠山歳三，吉野文彦
  • Organizer: 神奈川歯科大学学会 第 51 回総会
  • Place of Presentation: 横須賀
  • Year and Date: 2016-12-03
• [Presentation] ガン化学療法に併発する口内炎に対する糖転移ヘスペリジンの効果 (2016)
  • Author(s): 吉野文彦，吉田彩佳，小椋有香子，遠山歳三，高橋聡子，高橋俊介
  • Organizer: 神奈川歯科大学学会 第 51 回総会
  • Place of Presentation: 横須賀
  • Year and Date: 2016-12-03
• [Presentation] 赤外自由電子レーザーの口腔細菌に対する殺菌効果の検討 (2016)
  • Author(s): 遠山歳三，古屋田泰徳，藤岡 隼，佐々木悠，吉野文彦，吉田彩佳，渡辺清子，佐久間隆章，今井貴之，小野善弘，築山光一，浜田信城
  • Organizer: 神奈川歯科大学学会 第 51 回総会
  • Place of Presentation: 横須賀
  • Year and Date: 2016-12-03
• [Presentation] 新規根管消毒法に関する基礎的研究ー大気を利用したプラズマ発生装置を使用した根管消毒法の検討ー (2016)
  • Author(s): 平野頼是，林 誠，田村宗明，吉野文彦，吉田彩佳，小林慶美，井比陽奈，増渕光暁，小木曽文内
  • Organizer: 日本日本歯科保存2016年度春季学術大会(第144回)
  • Place of Presentation: 宇都宮
  • Year and Date: 2016-06-09
• [Presentation] Study of Blue-light Effect on rat palatal gingiva. (2016)
  • Author(s): Yoshida A, Yoshino F
  • Organizer: 45th Annual meeting of the AADR
  • Place of Presentation: Los Angeles, CA, USA.
  • Year and Date: 2016-03-16
  • Int'l Joint Research
• [Presentation] Disinfection for Endodontic Treatment by Ultrasound/LED Activation of H2O2 (2016)
  • Author(s): Ibi H, Hayashi, Yoshino F, Yoshida A, Kobayashi Y, Tamura M, Ochiai K, Ogiso B, Lee M
  • Organizer: 45th Annual meeting of the AADR
  • Place of Presentation: Los Angeles, CA, USA.
  • Year and Date: 2016-03-16
  • Int'l Joint Research
• [Presentation] 青色 LED 照射がラット口腔領域に及ぼす影響の検討. (2015)
  • Author(s): 吉田彩佳，小椋有香子，高橋俊介，高橋聡子，前畑洋次郎，宮本千央，堀 紀雄，遠山歳三，秦 光潤，前谷崇志，李 昌一，吉野文彦
  • Organizer: 第 15 回 日本抗加齢医学会総会
  • Place of Presentation: 福岡
  • Year and Date: 2015-05-29