Identification and validation of gastric cancer genes

• Project/Area Number: 15K21470
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pathological medical chemistry; Tumor biology
• Research Institution: Kanazawa University (2016); Kanazawa Medical University (2015)
• Principal Investigator: Takeda Haruna 金沢大学, がん進展制御研究所, 助教 (80647975)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: がん / マウスモデル / 消化管 / 遺伝子 / 挿入変異誘発法 / マウス / スクリーニング

Outline of Final Research Achievements

We identified 1026 gastric candidate driver genes by genome-wide SB transposon mutagenesis screening in mice. Genes in Wnt signaling and TGF-beta signaling were frequently mutated, showing that SB-induced gastric tumors model human gastric cancers. Comparative analysis for candidate gastric cancer genes between human and mice enriched 258 genes. These genes are very likely to be potent candidate cancer genes in the stomach. Our result will help developing new therapeutic targets to cure gastric cancers.

Research Products

• [Journal Article] Sleeping Beauty transposon mutagenesis identifies genes that cooperate with mutant Smad4 in gastric cancer development (2016)
  • Author(s): Haruna Takeda, Alistair G. Rust, Jerrold M. Ward, Christopher Chin Kuan Yew, Nancy A. Jenkins and Neal G. Copeland
  • Journal Title: Proc Natl Acad Sci USA Volume: 113 Pages: 2057-2065
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] トランスポゾン挿入変異を用いたがん形成に関与する遺伝子の同定 (2015)
  • Author(s): 武田はるな
  • Journal Title: 金沢医科大学雑誌 Volume: 40 Pages: 54-63
  • Peer Reviewed
• [Presentation] Sleeping beauty transposon identifies genes involved in colon cancer development (2017)
  • Author(s): 武田 はるな
  • Organizer: ALBERTA-JAPAN Agency For Medical Research And Development (AMED) Workshop For Medical Innovation
  • Place of Presentation: カルガリー・カナダ・ハイアットホテル
  • Year and Date: 2017-02-24
• [Presentation] Sleeping Beauty (SB)トランスポゾンによる大腸がん形成に関与する遺伝子の同定 (2016)
  • Author(s): 武田 はるな
  • Organizer: 第2回AMEDがん若手研究者ワークショップ
  • Place of Presentation: 東京・晴海グランドホテル
  • Year and Date: 2016-11-29
• [Presentation] Sleeping Beauty (SB)トランスポゾンによる大腸がん形成に関与する遺伝子の同定 (2016)
  • Author(s): 武田 はるな
  • Organizer: 第75回日本癌学会学術総会
  • Place of Presentation: 横浜・パシフィコ横浜
  • Year and Date: 2016-10-06
  • Int'l Joint Research / Invited
• [Presentation] Sleeping Beauty transposon mutagenesis identifies genes that cooperate with Smad4 loss in gastric cancer development (2016)
  • Author(s): Haruna Takeda
  • Organizer: Breakthroughs in cancer research:from biology to therapeutics
  • Place of Presentation: Maui, Hawaii
  • Year and Date: 2016-02-16
  • Int'l Joint Research
• [Presentation] Transposon mutagenesis identifies genes and evolutionary forces driving gastrointestinal tract tumor progression (2015)
  • Author(s): 武田はるな
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場（愛知県名古屋市）
  • Year and Date: 2015-10-08
  • Int'l Joint Research