Boron neutron capture therapy for clear cell sarcoma (CCS): Influence of expression of LAT1 in CCS on the cellular uptake of p-borono-L-phenylalanine
Project/Area Number |
15K21533
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Radiation science
Orthopaedic surgery
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Research Institution | Kobe Gakuin University |
Principal Investigator |
Andoh Tooru 神戸学院大学, 薬学部, 助手 (50639226)
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Research Collaborator |
Fujimoto Takuya 兵庫県立がんセンター, 整形外科
Sudo Tamotsu 兵庫県立がんセンター, 研究部
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Project Period (FY) |
2015-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Keywords | ホウ素中性子線捕捉療法 / 明細胞肉腫 / 軟部肉腫 / p-borono-L-phenylalanine / LAT1 / ホウ素中性子捕捉療法 / L-BPA / LAT-1 |
Outline of Final Research Achievements |
L-type amino acid transporter-1 (LAT1) carries p-borono-L-phenylalanine (L-BPA) into tumor cells, mediating the efficacy of BNCT. Malignant tumor cells preferentially take up L-BPA because its chemical structure is similar to that of tyrosine. Indeed, our previous study demonstrated that Clear cell sarcoma (CCS) has the ability to highly take up L-BPA in vitro and in vivo. However, difference of antitumor effect among CCS cell lines based on an uptake of L-BPA. Thus, BNCT utilizing L-BPA could be a novel clinical option to treat CCS, provided that this research could reveal the mechanism of uptake. LAT-1 was observed in each cell line by westan-blott method. The uptake of 10B in four CCS cell lines was inhibited by pretreatment with BCH. The inhibitory efficiency was 31.6 to 55.6%, each CCS cell line may correlate with expression level. These results indicated that LAT-1 was expressed in CCS cell lines and played a key role in uptake of L-BPA.
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Report
(3 results)
Research Products
(16 results)
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[Presentation] Evaluation of relationship between uptake of L-BPA in Clear Cell Sarcoma Cell Line and L-type amino acid transporter 1.2016
Author(s)
T. Andoh, T. Fujimoto, K. Wada, Y. Uchida, T. Sudo, H. Moritake, Alan L. Epstein, T. Takeuchi, Y. Fukumori, H. Ichikawa
Organizer
17th International Congress on Neutron Capture Therapy
Place of Presentation
Columbia, Missouri, America
Year and Date
2016-10-02
Related Report
Int'l Joint Research
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[Presentation] Boron neutron caputure therapy (BNCT) for axillary lymph node metastasis of breast cancer2016
Author(s)
T. Fujimoto, Y. Maekawa, S. Takao, A. Hori, T. Andoh, I. Fujita, Y. Sakurai, H. Tanaka, S. Masunaga, M. Imabori, K. kitayama, S. Hori, H. Ichikawa, K. Ono, M. Suzuki
Organizer
17th International Congress on Neutron Capture Therapy
Place of Presentation
Columbia, Missouri, America
Year and Date
2016-10-02
Related Report
Int'l Joint Research
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[Presentation] Evaluation of relationship between uptake of L-BPA in Clear Cell Sarcoma Cell Line and L-type amino acid transporter 12016
Author(s)
T. Andoh, T. Fujimoto, K. Wada, Y. Uchida, T. Sudo, H. Moritake, Alan L. Epstein, T. Takeuchi, Y. Fukumori, H. Ichikawa
Organizer
17th International Congress on Neutron Capture Therapy
Place of Presentation
Missouri, USA
Year and Date
2016-10-02
Related Report
Int'l Joint Research
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