Analysis of aging and carcinogenesis mechanism using replication incompletion model mice
| Project/Area Number |
15K21565
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
Risk sciences of radiation and chemicals
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| Research Institution | University of Occupational and Environmental Health, Japan |
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Principal Investigator |
KOHZAKI Masaoki 産業医科大学, 産業生態科学研究所, 助教 (90717977)
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| Research Collaborator |
MIYATA Hironori
OHTSUKA Masato
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | DNA複製 / 細胞周期チェックポイント / トランスジェニックマウス / コンディショナルノックアウト / 発がん / 老化 / 複製 / 細胞周期チェックポイ / マウスモデル / Cre-loxP / 抗がん剤耐性 / ノックアウト / p53 |
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| Outline of Final Research Achievements |
Inappropriate replication completion may cause genome instability, a characteristic of almost all human cancers. However, the direct relationship between incompletion of replication and cancer remains largely unknown. Here we sought to establish replication completion-related X gene knockout mice to address the hypothesis and confirm the phenotypes. Although we established gene X knockout mice as a mouse model for replication incompletion, we found that gene X is partially essential for viability and the viability depends on mouse strains. It was practically difficult for us to conduct several experiments with these mice. Hence, we changed strategy to make conventional conditional X gene knockout mice. At present, we obtained the floxed X gene mice.
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Report
(4 results)
Research Products
(8 results)
