| Project/Area Number |
15K21596
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Civil and environmental engineering
Environmental engineering and reduction of environmental burden
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| Research Institution | Kitasato University (2017)
Oita National College of Technology (2015-2016) |
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Principal Investigator |
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| Research Collaborator |
Sei Kazunari
Konishi Tadashi
Mekata Tohru
Hashimoto Reina
Jikumaru Atsushi
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 薬剤耐性菌 / 薬剤耐性遺伝子 / 下水処理場 / バンコマイシン / バンコマイシン耐性遺伝子 / 従属栄養細菌 / 耐性遺伝子 / バンコマイシン耐性菌 / ふん便性細菌 / 塩素処理 / バンコマイシン耐性腸球菌 / 最小発育阻止濃度試験 / リアルタイム定量PCR法 / リアルタイム定量PCR |
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| Outline of Final Research Achievements |
To clarify the behavior of antibiotic-resistant bacteria (ARB) and antibiotic-resistant genes (ARGs) in sewage water treatment plant, the presence of heterotrophic bacteria resistant to vancomycin and vanA and vanB, vancomycin resistant genes in each sewage water and sludge sample were determined. Heterotrophic bacteria resistant to vancomycin and vanA and vanB were detected from all sewage water and sludge samples even in the chlorinated water. Furthermore, in batch-type chlorination experiment of vancomycin-resistant enterococci (VRE) were carried out. Although VRE could be inactivated completely by chlorination, vanA gene could not be destroyed and remained in chlorinated water. Our results strongly indicated that sewage water treatment plant might play as pools of ARB and ARGs and discharge to water environments.
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