| Project/Area Number |
15K21607
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General medical chemistry
Molecular biology
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| Research Institution | Microbial Chemistry Research Foundation |
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Principal Investigator |
TAKIZAWA Naoki 公益財団法人微生物化学研究会, 微生物化学研究所, 研究員 (50448502)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | インフルエンザウイルス / 分節化ウイルス / RNAウイルス / RNA-RNA結合 / 分節化ゲノム |
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| Outline of Final Research Achievements |
The genome of influenza A virus consists of eight single-stranded negative-sense RNA segments. To be infectious virion, all eight segments need to be incorporated into a virion. However, the mechanism of how all eight segments are selectively incorporated into a virion is not known. To elucidate the mechanism, we try to construct a new system using chemical RNA cross-link reagent and next generation sequencing to reveal global RNA-RNA interactions between viral genome segments in virion. As a result, we find RNA-RNA interactions between specific segments in virion. This result will contribute to reveal the RNA interaction network between viral genome RNAs and the mechanism of specific incorporations of all eight segments.
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