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Structural and functional analyses of autophagy essencial protein Atg9

Research Project

Project/Area Number 15K21608
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Structural biochemistry
Cell biology
Research InstitutionMicrobial Chemistry Research Foundation

Principal Investigator

MATOBA Kazuaki  公益財団法人微生物化学研究会, 微生物化学研究所・構造生物学研究部, 研究員 (60613792)

Project Period (FY) 2015-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsオートファジー / 膜タンパク質
Outline of Final Research Achievements

To determine a structure of autophagy essential membrane protein, overexpression and solubilization conditions were established and the purity was high enough to be detected as a single band using CBB staining. Protein-Fab complex crystallization was performed, but structural analysis has not been the success. In order to observe the single particle by cryo-electron microscope, Nanodisc-protein-Fab complex was reconstituted. As a result, Fab bound particle was observed by negative staining.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (4 results)

All 2016 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Remarks (3 results)

  • [Journal Article] Structural mechanisms of receptor-mediated selective autophagy of aggregated aminopeptidase I.2016

    • Author(s)
      Akinori Yamasaki, Yasunori Watanabe, Wakana Adachi, Kuninori Suzuki, Kazuaki Matoba, Hiromi Kirisako, Hiroyuki Kumeta, Hitoshi Nakatogawa, Yoshinori Ohsumi, Fuyuhiko Inagaki and Nobuo N. Noda
    • Journal Title

      Cell Reports

      Volume: 16 Issue: 1 Pages: 19-27

    • DOI

      10.1016/j.celrep.2016.05.066

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Remarks] 微生物化学研究会

    • URL

      http://www.bikaken.or.jp/

    • Related Report
      2017 Annual Research Report
  • [Remarks] 微生物化学研究所

    • URL

      http://www.bikaken.or.jp/

    • Related Report
      2016 Research-status Report
  • [Remarks] 微生物化学研究所

    • URL

      http://www.bikaken.or.jp/pr/index.html

    • Related Report
      2015 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2019-03-29  

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