Structural and functional analyses of autophagy essencial protein Atg9
Project/Area Number |
15K21608
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Structural biochemistry
Cell biology
|
Research Institution | Microbial Chemistry Research Foundation |
Principal Investigator |
MATOBA Kazuaki 公益財団法人微生物化学研究会, 微生物化学研究所・構造生物学研究部, 研究員 (60613792)
|
Project Period (FY) |
2015-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | オートファジー / 膜タンパク質 |
Outline of Final Research Achievements |
To determine a structure of autophagy essential membrane protein, overexpression and solubilization conditions were established and the purity was high enough to be detected as a single band using CBB staining. Protein-Fab complex crystallization was performed, but structural analysis has not been the success. In order to observe the single particle by cryo-electron microscope, Nanodisc-protein-Fab complex was reconstituted. As a result, Fab bound particle was observed by negative staining.
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Report
(4 results)
Research Products
(4 results)