Development of visualization method of phosphorylation dynamics of beta-catenin for the study of in vivo Wnt signal regulation machinery
| Project/Area Number |
15K21625
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Developmental biology
General anatomy (including histology/embryology)
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Takai Akira 国立研究開発法人理化学研究所, 生命システム研究センター, 基礎科学特別研究員 (60637205)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | Wntシグナル / シグナル伝達 / プローブ開発 / 発生生物学 / ライブイメージング |
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| Outline of Final Research Achievements |
In this research, we aimed to develop novel visualization probes of phosphorylation dynamics of β-catenin for real-time and quantitative analysis of Wnt signaling at the level of β-catenin protein. As a result, we succeeded in the development of several probes with higher responsiveness to Wnt signaling, compared with conventional Wnt probe. After further improvement of these probes in S/N and sensitivity, we will apply these probes to quantitative analysis of in vivo Wnt signaling and study its regulation machinery.
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Report
(3 results)
Research Products
(5 results)
