Study on the developmental basis behind the evolutionary changes of musculoskeletal connections in vertebrates

• Project/Area Number: 15K21628
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Stratigraphy/Paleontology; General anatomy (including histology/embryology)
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Hirasawa Tatsuya 国立研究開発法人理化学研究所, 倉谷形態進化研究室, 研究員 (60585793)
• Research Collaborator: FUJIMOTO Satoko
• Project Period (FY): 2015-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 進化 / 発生 / 脊椎動物 / 筋 / 腱 / 化石 / 脊椎動物進化 / 進化発生学 / 古生物学 / 筋骨格系

Outline of Final Research Achievements

The development of musculoskeletal connections in vertebrates and its relationship with the evolutionary changes were studied. We analyzed the developmental process of tendons in the forelimbs of four tetrapod taxa, based on Scx expression patterns, and built a foundation of future researches on the relationship between developmental variability and evolvability. In addition, as an unexpected result, we found that in the evolution towards the turtles, the pectoralis muscle released the connection with the skeletal system and made a connection with the dermis covering the plastron.

Research Products

• [Journal Article] The neural crest and evolution of the head/trunk interface in vertebrates (2018)
  • Author(s): Kuratani S, Kusakabe R, Hirasawa T
  • Journal Title: Developmental Biology Volume: in press Pages: 30922-30923
  • DOI: 10.1016/j.ydbio.2018.01.017
  • Peer Reviewed / Open Access
• [Journal Article] 1.Anatomical integration of the sacral-hindlimb unit coordinated by GDF11 underlies variation in hindlimb positioning in tetrapods. (2017)
  • Author(s): Matsubara Y., Hirasawa T., Egawa S., Hattori A., Suganuma T., Kohara Y., Nagai T., Tamura K., Kuratani S., Kuroiwa A., Suzuki T
  • Journal Title: Nature Ecology and Evolution Volume: １ Issue: 9 Pages: 1392-1399
  • DOI: 10.1038/s41559-017-0247-y
  • Peer Reviewed
• [Journal Article] Outflow tract septation and the aortic arch system in reptiles: lessons for understanding the mammalian heart (2017)
  • Author(s): Poelmann RE, Gittenberger-de Groot AC, Biermans MWM, Dolfing AI, Jagessar A, van Hattum S, Hoogenboom A, Wisse LJ, Vicente-Steijn R, de Bakker MAG, Vonk FJ, Hirasawa T, Kuratani S, Richardson MK
  • Journal Title: EvoDevo Volume: 8 Issue: 1 Pages: 9-9
  • DOI: 10.1186/s13227-017-0072-z
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] News & Views: Getting the measure of a monster. (2016)
  • Author(s): Kuratani, S., and Hirasawa, T
  • Journal Title: Nature Volume: 532 Issue: 7600 Pages: 447-448
  • DOI: 10.1038/nature17885
  • Peer Reviewed
• [Journal Article] Palaeospondylus as a primitive hagfish. (2016)
  • Author(s): Hirasawa, T., Oisi, Y., and Kuratani, S.
  • Journal Title: Zool. Lett. Volume: 2 Issue: 1 Pages: 20-20
  • DOI: 10.1186/s40851-016-0057-0
  • Peer Reviewed / Open Access
• [Journal Article] On the vagal cardiac nerves, with special reference to the early evolution of the head-trunk-interface. (2016)
  • Author(s): Higashiyama, H., Hirasawa, T., Oisi, Y., Sugahara, F., Hyodo, S., Kanai, Y., and Kuratani, S.
  • Journal Title: J. Morphol. Volume: 277 Issue: 9 Pages: 1146-1158
  • DOI: 10.1002/jmor.20563
  • Peer Reviewed
• [Journal Article] Expansion of the neck reconstituted the shoulder-diaphragm in amniote evolution (2016)
  • Author(s): Hirasawa T, Fujimoto S, Kuratani S
  • Journal Title: Development, Growth & Differentiation Volume: 58 Issue: 1 Pages: 143-153
  • DOI: 10.1111/dgd.12243
  • Peer Reviewed / Open Access
• [Journal Article] Developmental mechanisms of the tympanic membrane in mammals and non-mammalian amniotes (2016)
  • Author(s): Takechi, M., Kitazawa, T., Hirasawa, T., Hirai, T., Iseki, S., Kurihara, H., Kuratani, S.
  • Journal Title: Congenital Anomalies Volume: 56 Issue: 1 Pages: 12-17
  • DOI: 10.1111/cga.12132
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Comparative analysis of pleurodiran and cryptodiran turtle embryos depicts the molecular ground pattern of the turtle carapacial ridge (2015)
  • Author(s): Pascual-Anaya J, Hirasawa T, Sato I, Kuraku S, Kuratani S
  • Journal Title: International Journal of Developmental Biology Volume: 58 Issue: 10-11-12 Pages: 743-750
  • DOI: 10.1387/ijdb.140296jp
  • Peer Reviewed
• [Journal Article] Developmental genetic bases behind the independent origin of the tympanic membrane in mammals and diapsids. (2015)
  • Author(s): Kitazawa T, Takechi M, Hirasawa T, Adachi N, Narboux-Neme N, Kume H, Maeda K, Hirai T, Miyagawa-Tomita S, Kurihara Y, Hitomi J, Levi G, Kuratani S, Kurihara H
  • Journal Title: Nature Communications Volume: 6 Issue: 1 Pages: 6853-6853
  • DOI: 10.1038/ncomms7853
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Evolutionary developmental perspectives on the variability of limb muscle connections (2018)
  • Author(s): Hirasawa T
  • Organizer: 1st AsiaEvo Conference (Shenzhen, China)
  • Int'l Joint Research / Invited
• [Presentation] Evolutionary origin of the turtle shell accompanied with the loss of the sternum (2018)
  • Author(s): Hirasawa T, Kuratani S
  • Organizer: 6th Turtle Evolution Symposium (Tokyo)
  • Int'l Joint Research
• [Presentation] Mode in Evolution of Tetrapod Musculoskeletal Systems: A case Study of an Evolutionary Breakthrough, the Diaphragm (2017)
  • Author(s): Hirasawa T
  • Organizer: Zoological Letters Symposium: Early Evolution and Diversity of Vertebrates
  • Place of Presentation: 理化学研究所多細胞システム形成研究センター（兵庫県・神戸市）
  • Year and Date: 2017-03-18
  • Int'l Joint Research / Invited
• [Presentation] Evolutionary origin of the diaphragm (2016)
  • Author(s): Hirasawa T, Fujimoto S, Kuratani S
  • Organizer: 76th Annual Meeting, Society of Vertebrate Paleontology
  • Place of Presentation: Salt Lake City, UT
  • Year and Date: 2016-10-28
  • Int'l Joint Research / Invited
• [Presentation] Developmental basis behind the evolutionary origin of the diaphragm (2016)
  • Author(s): Hirasawa T, Fujimoto S, Kuratani S
  • Organizer: 11th International Congress of Vertebrate Morphology
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2016-07-02
  • Int'l Joint Research
• [Presentation] 古生物学と発生学をつなぐ (2015)
  • Author(s): 平沢達矢
  • Organizer: 京都大学大学院理学系研究科生物科学専攻生物多様性コロキウム
  • Place of Presentation: 京都大学（京都）
  • Year and Date: 2015-11-10
  • Invited
• [Book] 手の百科事典 (2017)
  • Author(s): バイオメカニズム学会（「手の起源」、「両生類」、「恐竜と鳥の指骨の相同」を担当）
  • Total Pages: 608
  • Publisher: 朝倉書店
  • ISBN: 9784254102673