Dendritic cell-based systemic vaccination induces lung resident memory Th17 that contributes to long-term protection against pulmonary fungal infection
Project/Area Number |
15K21644
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Immunology
Bacteriology (including mycology)
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Research Institution | National Institute of Infectious Diseases |
Principal Investigator |
Ueno Keigo 国立感染症研究所, 真菌部, 主任研究官 (10550220)
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Research Collaborator |
KINJO Yuki 国立感染症研究所, 真菌部3室, 室長
IWAKURA Yoichiro 東京理科大学, 生命医科学研究所, 教授
SHIBUYA Kazutoshi 東邦大学, 医学部・病院病理学講座, 教授
TAKATSUKA Yuki 国立感染症研究所, 真菌部3室, 研究員
ABE Masahiro 国立感染症研究所, 真菌部3室, 協力研究員
OTANI Yoshiko 国立感染症研究所, 真菌部3室, 実習生
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Project Period (FY) |
2015-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | Tissue Resident Memory / CD4+ Memory T cells / vaccine / fungal infection / Cryptococcus gattii / cytokine / granuloma / neutrophils / Th17 / Fungal infection / Vaccine |
Outline of Final Research Achievements |
We analyzed vaccine-mediated immunity using dendritic cell-based (DC) vaccine to elucidate the protective immunity against a fungal pathogen Cryptococcus gattii. In this study, we identified the novel lung resident memory Th17 cells (Lung TRM17) in the immunized mice, which has characteristic marker profiles and a long-lived trait. DC vaccine significantly ameliorated fungal burden and survival rate after infection, and it upregulated IL-17A production in lungs of immunized mice after infection. The protective effect of DC vaccine was significantly reduced in IL-17A deficient mice, but not in mice treated by the immunosuppressive agents FTY720. The infection control in the immunized mice was correlated with IL-17A dependent neutrophil activation and accumulation in the lungs after infection. These results suggested the Lung TRM17 contributed to the infection control against C. gattii in the vaccinated mice.
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Report
(3 results)
Research Products
(17 results)
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[Presentation] 肺常在性記憶型T細胞は高病原性クリプトコックス症の感染制御に寄与するか?2016
Author(s)
上野 圭吾, 金城 雄樹, 浦井 誠, 栃木 直文, 篠崎 稔, 清水 公徳, 亀井 克彦, 大野 秀明, 二木 芳人, 澁谷 和俊, 宮﨑 義継
Organizer
第60回 日本医真菌学会総会
Place of Presentation
東京
Year and Date
2016-10-01
Related Report
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[Presentation] 樹状細胞ワクチンを用いた高病原性Cryptcoccus gattiiに対する感染制御機構の解析2015
Author(s)
上野 圭吾, 金城 雄樹, 浦井 誠, 大久保 陽一郎, 清水 公徳, 金子 幸弘, 亀井 克彦, 大野 秀明, 二木 芳人, 澁谷 和俊, 宮﨑 義継
Organizer
第59回 日本医真菌学会総会
Place of Presentation
札幌
Year and Date
2015-10-08
Related Report
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