| Project/Area Number |
15K21644
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Immunology
Bacteriology (including mycology)
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
Ueno Keigo 国立感染症研究所, 真菌部, 主任研究官 (10550220)
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| Research Collaborator |
KINJO Yuki 国立感染症研究所, 真菌部3室, 室長
IWAKURA Yoichiro 東京理科大学, 生命医科学研究所, 教授
SHIBUYA Kazutoshi 東邦大学, 医学部・病院病理学講座, 教授
TAKATSUKA Yuki 国立感染症研究所, 真菌部3室, 研究員
ABE Masahiro 国立感染症研究所, 真菌部3室, 協力研究員
OTANI Yoshiko 国立感染症研究所, 真菌部3室, 実習生
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | Tissue Resident Memory / CD4+ Memory T cells / vaccine / fungal infection / Cryptococcus gattii / cytokine / granuloma / neutrophils / Th17 / Fungal infection / Vaccine |
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| Outline of Final Research Achievements |
We analyzed vaccine-mediated immunity using dendritic cell-based (DC) vaccine to elucidate the protective immunity against a fungal pathogen Cryptococcus gattii. In this study, we identified the novel lung resident memory Th17 cells (Lung TRM17) in the immunized mice, which has characteristic marker profiles and a long-lived trait. DC vaccine significantly ameliorated fungal burden and survival rate after infection, and it upregulated IL-17A production in lungs of immunized mice after infection. The protective effect of DC vaccine was significantly reduced in IL-17A deficient mice, but not in mice treated by the immunosuppressive agents FTY720. The infection control in the immunized mice was correlated with IL-17A dependent neutrophil activation and accumulation in the lungs after infection. These results suggested the Lung TRM17 contributed to the infection control against C. gattii in the vaccinated mice.
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