| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
|
|---|
| Outline of Final Research Achievements |
Severe fever with thrombocytopenia syndrome (SFTS) is caused by SFTS virus (SFTSV). SFTSV belongs to genus Phlebovirus, family Phenuiviridae. In this study, we tried to establish reverse genetics system for SFTSV to apply this system to a high-throughput drug screening system and functional analysis of viral proteins. However, we couldn’t establish the system. Alternatively, we analyzed the characteristics of SFTSV glycoprotein and developed a new neutralization assay of SFTSV. Furthermore, we analyzed the function of nucleoprotein which is essential for viral genome replication.
Lymphocytic choriomeningitis virus (LCMV) belongs to family Arenaviridae. In this study, we tried to elucidate the role of untranslated regions (UTRs) of LCMV S segment genome. We found that introduction of mutations in the UTR in S segment resulted in attenuation of LCMV. These results suggested LCMVs with mutated UTRs could be a candidate of attenuated LCMV vaccine.
|
