| Project/Area Number |
15K21646
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor therapeutics
Experimental pathology
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| Research Institution | National Cancer Center Japan |
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Principal Investigator |
Naito Yutaka 国立研究開発法人国立がん研究センター, 研究所, 外来研究員 (70738210)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | Exosome / microRNA / スキルス胃癌 / 癌関連線維芽細胞 / 代謝 / 癌微小環境 / メタボローム |
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| Outline of Final Research Achievements |
In the present study, we use co-culture system of fibroblasts with high-metastatic scirrhous gastric cancer (SGC) cells or low-metastatic SGC cells as a model to understand how to create favorable microenvironments for cancer metastasis. By comparing transcriptome profiles of fibroblasts co-cultured with high- or low-metastatic SGC cells, it revealed that glucose metabolism in fibroblasts was reprogrammed by co-culture with high-metastatic SGC cells. We also found that high-metastatic DGC-derived extracellular vesicles (EVs) dominantly induced inflammatory cytokines through TGF-β independent manner. Under co-culture condition also strongly induced the expressions of glycolysis-related genes and decreased the expression of oxidative phosphorylation-related genes in high-metastatic SGC cells. Thus, our finding suggests the cellular crosstalk between cancer cells and fibroblasts via EVs in SGC microenvironment.
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