Role of TSLP in skin inflammation at chronic stage

• Project/Area Number: 15K21649
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Immunology; Dermatology
• Research Institution: National Center for Global Health and Medicine
• Principal Investigator: KITAJIMA MASAYUKI 国立研究開発法人国立国際医療研究センター, その他部局等, 上級研究員 (00401000)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 慢性炎症 / アレルギー性皮膚炎 / TSLP

Outline of Final Research Achievements

The cytokine thymic stromal lymphopoietin (TSLP) has been implicated in the development and progression of allergic inflammation in humans and mice. However, its role in chronic skin inflammation has not been elucidated. To understand the role of TSLP in chronic phase of skin inflammation, we established novel skin inflammation mouse models utilizing TSLP receptor (TSLPR)-deficient mice and conditional TSLPR-deficient mice in the current study. Results from these models revealed that TSLP has pivotal roles in skin inflammation in chronic stage. In addition, we found that dendritic cells and T cells are the target of TSLP in chronic skin inflammation by using cell type specific conditional TSLPR-deficient mice. Additionally, analysis from TSLP-reporter mice indicated that dendritic cells and monocytoid cells produced TSLP in chronic inflamed skin region. Collectively, our current results proved the essential requirement of TSLP in chronic skin inflammation.

Research Products

• [Int'l Joint Research] Benaroya Research Institute(米国)
• [Journal Article] Baculovirus directly activates murine NK cells via TLR9 (2017)
  • Author(s): Moriyama, T., Suzuki, T., Chang, Mo., Kitajima, M., Takaku, H.
  • Journal Title: The Journal of Cancer Gene and Cellular Therapies Volume: - Issue: 4 Pages: 175-179
  • DOI: 10.1038/cgt.2017.2
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] A Histone Methyltransferase ESET Is Critical for T Cell Development (2016)
  • Author(s): Takikita, S. Muro, R. Takai, T. Otsubo, T. Kawamura, Y. I. Dohi, T. Oda, H. Kitajima, M. Oshima, K. Hattori, M. Endo, T. A. Toyoda, T. Weis, J. Shinkai, Y. Suzuki, H.
  • Journal Title: J Immunol Volume: 197 Issue: 6 Pages: 2269-2279
  • DOI: 10.4049/jimmunol.1502486
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Baculovirus infection of human monocyte-derived dendritic cells restricts HIV-1 replication. (2015)
  • Author(s): Oo Chang, M., Suzuki, T., Kitajima, M., Takaku, H.
  • Journal Title: AID Res Hum Retrovirues Volume: 10 Issue: 10 Pages: 1023-1031
  • DOI: 10.1089/aid.2015.0060
  • Peer Reviewed
• [Presentation] NAD(P)H:quinone oxidoreductase (Nqo1) regulates irritant contact dermatitis through homeostasis of epidermal γδ T cells. (2016)
  • Author(s): M Kitajima, A Kimura, H Suzuki
  • Organizer: 第４５回 日本免疫学会総会・学術集会
  • Place of Presentation: 沖縄
  • Year and Date: 2016-12-05
• [Presentation] NAD(P)H:quinone oxidoreductase (Nqo1) regulates irritant contact dermatitis through homeostasis of epidermal γδ T cells. (2016)
  • Author(s): M Kitajima, A Kimura, H Suzuki
  • Organizer: Immunology 2016, The American Association of Immunologists Annual meeting
  • Place of Presentation: Seattle
  • Year and Date: 2016-05-13
  • Int'l Joint Research
• [Presentation] NAD(P)H:quinone oxidoreductase (Nqo1) regulates irritant contact dermatitis (2015)
  • Author(s): Masayuki Kitajima, Akihiro Kimura, Harumi Suzuki
  • Organizer: 第４４回 日本免疫学会学術集会
  • Place of Presentation: 札幌市
  • Year and Date: 2015-11-20