• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of sensitive and selective LC-MS method for protein and assessment method for deamidation of protein using carboxy group derivatization

Research Project

Project/Area Number 15K21662
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Physical pharmacy
Analytical chemistry
Research InstitutionFukuoka University (2018)
National Institute of Advanced Industrial Science and Technology (2015-2017)

Principal Investigator

Sakaguchi Yohei  福岡大学, 薬学部, 助教 (10712507)

Project Period (FY) 2015-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2018: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
KeywordsLC-MS / タンパク質 / ペプチド / アミノ酸 / 誘導体化 / カルボキシル基
Outline of Final Research Achievements

In this study, a carboxyl groups derivatization LC-MS method for proteins or peptides was developed. By derivatizing the highly polar carboxyl group, it was possible to efficiently improve the sensitivity in LC-MS analysis. Furthermore, deamidation, which is a major degradation of proteins, is considered difficult to evaluate in LC-MS analysis because the mass hardly changes before and after degradation. However using this method it was possible to change the structure clearly before and after deterioration, and it was possible to evaluate easily.

Academic Significance and Societal Importance of the Research Achievements

新規臨床検査項目や新規バイオ医薬品が年々増加しているなかで,それらを安全に信頼して利用していただくためにも,タンパク質の構造変化をとらえる分析手法が必須だと考える。本研究において開発した分析法は、LC-MS分析におけるタンパク質またはペプチドの高感度化と、これまで正確な評価が困難だった脱アミド化を容易に評価することを可能とした。

Report

(5 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (19 results)

All 2018 2017 2016 2015

All Journal Article (5 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 4 results,  Acknowledgement Compliant: 1 results) Presentation (14 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results)

  • [Journal Article] Isotope-dilution liquid chromatography-tandem mass spectrometry for sensitive quantification of human insulin in serum using derivatization-technique2017

    • Author(s)
      Sakaguchi Yohei、Kinumi Tomoya、Takatsu Akiko
    • Journal Title

      Analytical Biochemistry

      Volume: 537 Pages: 26-32

    • DOI

      10.1016/j.ab.2017.08.019

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Quantification of peptides using N-terminal isotope coding and C-terminal derivatization for sensitive analysis by micro liquid chromatography-tandem mass spectrometry.2016

    • Author(s)
      Yohei SAKAGUCHI, Tomoya KINUMI, Akiko TAKATSU
    • Journal Title

      Journal of mass spectrometry

      Volume: 51 Issue: 12 Pages: 1111-1119

    • DOI

      10.1002/jms.3845

    • Related Report
      2017 Research-status Report 2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Quantification of glycated N-terminal peptide of hemoglobin using derivatization for multiple functional groups of amino acids followed by liquid chromatography/tandem mass spectrometry.2016

    • Author(s)
      Sakaguchi Y, Kinumi T, Yamazaki T, Takatsu A.
    • Journal Title

      Biomed Chromatogr

      Volume: 30 Issue: 2 Pages: 280-284

    • DOI

      10.1002/bmc.3524

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Selective and sensitive liquid chromatographic determination method of 5-hydroxyindoles with fluorous and fluorogenic derivatization2015

    • Author(s)
      Yohei Sakaguchi, Jun Ikenaga, Hideyuki Yoshida, Tadashi Hayama, Miki Itoyama, Kenichiro Todoroki, Osamu Imakyure, Masatoshi Yamaguchi, Hitoshi Nohta
    • Journal Title

      Journal of Pharmaceutical and Biomedical Analysis

      Volume: 114 Pages: 348-354

    • DOI

      10.1016/j.jpba.2015.06.003

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] 二官能基誘導体化とマイクロLC-MS/MSによる生理活性ペプチド類の高感度分析2015

    • Author(s)
      坂口洋平、絹見朋也、高津章子
    • Journal Title

      Chromatography

      Volume: 36 Pages: 52-52

    • Related Report
      2015 Research-status Report
  • [Presentation] フルオラス誘導体化LC-MS法による貝毒成分のオカダ酸及びドウモイ酸の分析2018

    • Author(s)
      赤穂健太,吉田秀幸,坂口洋平,古賀鈴依子,能田 均
    • Organizer
      第36回九州分析化学若手の会夏季セミナー
    • Related Report
      2018 Annual Research Report
  • [Presentation] キヌレニン鏡像異性体を対象とした高選択的二次元HPLC分析法の開発2018

    • Author(s)
      甲斐永美里,古賀鈴依子,坂口洋平,三田真史,井手友美,吉田秀幸,能田 均,濵瀬健司
    • Organizer
      第36回九州分析化学若手の会夏季セミナー
    • Related Report
      2018 Annual Research Report
  • [Presentation] ヒト血漿中クルクミンのフルオラス誘導体化LC-MS/MS分析2018

    • Author(s)
      濱岡祐司,矢野恵奈,巴山忠,古賀鈴依子,坂口洋平,吉田秀幸,能田均
    • Organizer
      第31回バイオメディカル分析科学シンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] パーフルオロポリエーテルカルボン酸の金属塩を利用したリン脂質の選択的抽出及びLC-MS/MS分析2018

    • Author(s)
      田坂友里恵,矢野恵奈,巴山忠,古賀鈴依子,坂口洋平,吉田秀幸,能田均
    • Organizer
      第31回バイオメディカル分析科学シンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] 蛍光誘導体化HPLC法による二枚貝中オカダ酸の分析2018

    • Author(s)
      吉田秀幸,河村梨那,中山絵梨奈,古賀鈴依子,矢野(清川)恵奈,坂口洋平,山口政俊,能田均
    • Organizer
      日本分析化学会第67年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 多次元HPLCを用いる代謝関連キラルアミノ酸の高選択的分析法開発2018

    • Author(s)
      古賀鈴依子,甲斐永美里,篠島朋子,三田真史,井手友美,坂口洋平,吉田秀幸,能田 均,濵瀬健司
    • Organizer
      第14回D-アミノ酸学会学術講演会
    • Related Report
      2018 Annual Research Report
  • [Presentation] フルオラス誘導体化LC-MS法による二枚貝中のオカダ酸分析2018

    • Author(s)
      赤穂 健太、坂口 洋平、河村 梨那、吉田 秀幸、古賀 鈴依子、能田 均
    • Organizer
      フルオラス科学研究会第11回シンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] カルボキシル基誘導体化LC-MS法によるタンパク質の脱アミド化評価法の開発2018

    • Author(s)
      川末慎葉,坂口洋平,古賀鈴依子,吉田秀幸,能田均
    • Organizer
      日本薬学会第139年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 同位体希釈質量分析法を用いたヒトインスリン認証標準物質の開発2018

    • Author(s)
      坂口洋平
    • Organizer
      第31回九州分析化学若手の会 春の講演会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Development of a carboxyl group derivatization LC method for monitoring the deamidation of protein2017

    • Author(s)
      Yohei Sakaguchi, Tomoya Kinumi, Akiko Takatsu
    • Organizer
      HPLC2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] カルボキシル基誘導体化LCを用いたタンパク質脱アミド化の評価法の開発2016

    • Author(s)
      坂口洋平、絹見朋也、高津章子
    • Organizer
      日本分析化学会第65年会
    • Place of Presentation
      北海道大学、札幌市
    • Year and Date
      2016-09-14
    • Related Report
      2016 Research-status Report
  • [Presentation] A dual functional-group derivatization liquid chromatography-tandem mass spectrometry method; application for quantification of human insulin using enzymatic digestion2016

    • Author(s)
      Yohei SAKAGUCHI, Tomoya KINUMI, Akiko TAKATSU
    • Organizer
      HPLC 2016, 44th International Symposium on High Performance Liquid Phase Separations and Related Techniques
    • Place of Presentation
      San Francisco Marriott Marquis、サンフランシスコ、アメリカ
    • Year and Date
      2016-06-19
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 二官能基誘導体化とマイクロLC-MS/MSによる生理活性ペプチド類の高感度分析2015

    • Author(s)
      坂口洋平、絹見朋也、高津章子
    • Organizer
      第26回クロマトグラフィー科学会議
    • Place of Presentation
      福岡市
    • Year and Date
      2015-11-11
    • Related Report
      2015 Research-status Report
  • [Presentation] 二官能基誘導体化-LC-MS/MSによる生理活性ペプチド類の高感度分析2015

    • Author(s)
      坂口洋平、絹見朋也、高津章子
    • Organizer
      第63回質量分析総合討論会
    • Place of Presentation
      つくば市
    • Year and Date
      2015-06-17
    • Related Report
      2015 Research-status Report

URL: 

Published: 2015-04-16   Modified: 2020-03-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi