High throughput assay system for PM2.5 with bioluminescent cell arrays(Fostering Joint International Research)
Project/Area Number |
15KK0029
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Research Category |
Fund for the Promotion of Joint International Research (Fostering Joint International Research)
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Allocation Type | Multi-year Fund |
Research Field |
Green/Environmental chemistry
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Research Institution | National Institute of Advanced Industrial Science and Technology |
Principal Investigator |
Sung Bae Kim 国立研究開発法人産業技術総合研究所, エネルギー・環境領域, 主任研究員 (60470043)
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Project Period (FY) |
2016 – 2019
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Project Status |
Completed (Fiscal Year 2019)
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Budget Amount *help |
¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000)
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Keywords | 生物発光 / 可視化 / イメージング / バイオアッセイ / ルシフェラーゼ / 近赤外線 / 発光基質 / 発光酵素 / 発光プローブ / 生理活性物質 / BRET / 蛋白質ー蛋白質間相互作用 / 発光 / リガンド / 化学物質 / 生体影響 / 分子イメージング / セレンテラジン / 一分子型生物発光プローブ / 分子認識 / 環境分析 / 生体分子 |
Outline of Final Research Achievements |
A broad spectrum of coworks was conducted ranging from synthesis of multifunctional luciferins and probes to imaging of mammalian cells and animals, through collaboration with MIPS, Stanford School of Medicine. In specific, we developed luciferase-selective luciferins, fluorophore-linked luciferins, TBET luciferins, and the 40 series of artificial luciferases as basic ingredients. We applied the basic materials to the development of retinoic acid-sensing probes, BRET9-type probes, and near infrared imaging system. The probes are even proven to be useful for animal imaging. We further improved a multichannel light sensing system. The present project was a precious chance to expend the research territory to the world through the international collaboration.
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Academic Significance and Societal Importance of the Research Achievements |
人工・天然化合物は数年以内に1億種を超え、化学物質の影響がただ一つの国に留まらない時代である。本研究期間中、本研究者はアメリカに渡り、「化学物質の生理活性」を生物発光で評価する研究を行い多数の国際共著論文を出版できた。世界の化学物質の流通量も年々増えている中で、本共同研究で行った「化学物質に起因する病理現象の可視化イメージング」は、環境・医学分野の新たな融合の可能性を試すものであり、付随する人的ネットワークの構築は今後の相互研究協力関係の拡大に資するものであった。滞在したスタンフォード大学(シリコンバレー)での産学連携活動を現場で見るチャンスでもあった。
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Report
(5 results)
Research Products
(41 results)
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[Journal Article] Biothiol-Activatable Bioluminescent Coelenterazine Derivative for Molecular Imaging in Vitro and in Vivo2019
Author(s)
Nanako Nomura, Ryo Nishihara, Takahiro Nakajima, Takahiro Nakajima, Sung Bae Kim, Naoko Iwasawa, Yuki Hiruta, Shigeru Nishiyama, Moritoshi Sato, Daniel Citterio, Koji Suzuki
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Journal Title
Anal. Chem.
Volume: 91(15)
Issue: 15
Pages: 9546-9553
DOI
Related Report
Peer Reviewed
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[Journal Article] Highly bright and stable NIR-BRET with blue-shifted coelenterazine derivatives for deep-tissue imaging of molecular events in vivo2019
Author(s)
Ryo Nishihara, Ramasamy Paulmurugan, Takahiro Nakajima, Eiji Yamamoto, Arutselvan Natarajan, Rayhaneh Afjei, Yuki Hiruta, Naoko Iwasawa, Shigeru Nishiyama, Daniel Citterio, Moritoshi Sato, Sung Bae Kim and Koji Suzuki
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Journal Title
Theranostics
Volume: 9(9)
Issue: 9
Pages: 2646-2661
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Azide- and Dye-Conjugated Coelenterazine Analogues for a Multiplex Molecular Imaging Platform2018
Author(s)
Ryo Nishihara, Emi Hoshino, Yoshiki Kakudate, Satoshi Kishigami, Naoko Iwasawa, Shin-ichi Sasaki, Takahiro Nakajima, Moritoshi Sato, Shigeru Nishiyama, Daniel Citterio, Koji Suzuki, Sung Bae Kim
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Journal Title
Bioconjugate Chemistry (ACS)
Volume: 印刷中
Issue: 6
Pages: 1922-1931
DOI
Related Report
Peer Reviewed
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[Patent(Industrial Property Rights)] 新規セレンテラジン化合物及びその用途2017
Inventor(s)
鈴木, チッテリオ, 西原, 金 誠培, 佐藤, 中嶋
Industrial Property Rights Holder
鈴木, チッテリオ, 西原, 金 誠培, 佐藤, 中嶋
Industrial Property Rights Type
特許
Industrial Property Number
2017-055985
Filing Date
2017-03-22
Related Report
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