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High throughput assay system for PM2.5 with bioluminescent cell arrays(Fostering Joint International Research)

Research Project

Project/Area Number 15KK0029
Research Category

Fund for the Promotion of Joint International Research (Fostering Joint International Research)

Allocation TypeMulti-year Fund
Research Field Green/Environmental chemistry
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

Sung Bae Kim  国立研究開発法人産業技術総合研究所, エネルギー・環境領域, 主任研究員 (60470043)

Project Period (FY) 2016 – 2019
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000)
Keywords生物発光 / 可視化 / イメージング / バイオアッセイ / ルシフェラーゼ / 近赤外線 / 発光基質 / 発光酵素 / 発光プローブ / 生理活性物質 / BRET / 蛋白質ー蛋白質間相互作用 / 発光 / リガンド / 化学物質 / 生体影響 / 分子イメージング / セレンテラジン / 一分子型生物発光プローブ / 分子認識 / 環境分析 / 生体分子
Outline of Final Research Achievements

A broad spectrum of coworks was conducted ranging from synthesis of multifunctional luciferins and probes to imaging of mammalian cells and animals, through collaboration with MIPS, Stanford School of Medicine. In specific, we developed luciferase-selective luciferins, fluorophore-linked luciferins, TBET luciferins, and the 40 series of artificial luciferases as basic ingredients. We applied the basic materials to the development of retinoic acid-sensing probes, BRET9-type probes, and near infrared imaging system. The probes are even proven to be useful for animal imaging. We further improved a multichannel light sensing system. The present project was a precious chance to expend the research territory to the world through the international collaboration.

Academic Significance and Societal Importance of the Research Achievements

人工・天然化合物は数年以内に1億種を超え、化学物質の影響がただ一つの国に留まらない時代である。本研究期間中、本研究者はアメリカに渡り、「化学物質の生理活性」を生物発光で評価する研究を行い多数の国際共著論文を出版できた。世界の化学物質の流通量も年々増えている中で、本共同研究で行った「化学物質に起因する病理現象の可視化イメージング」は、環境・医学分野の新たな融合の可能性を試すものであり、付随する人的ネットワークの構築は今後の相互研究協力関係の拡大に資するものであった。滞在したスタンフォード大学(シリコンバレー)での産学連携活動を現場で見るチャンスでもあった。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (41 results)

All 2020 2019 2018 2017 2016 2015 Other

All Int'l Joint Research (1 results) Journal Article (19 results) (of which Int'l Joint Research: 7 results,  Peer Reviewed: 18 results,  Open Access: 6 results,  Acknowledgement Compliant: 9 results) Presentation (10 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results) Book (1 results) Remarks (2 results) Patent(Industrial Property Rights) (8 results) (of which Overseas: 5 results)

  • [Int'l Joint Research] スタンフォード大学医学部(米国)2017

    • Year and Date
      2017-03-20
    • Related Report
      2019 Annual Research Report
  • [Journal Article] Highly Sensitive Eight-Channel Light Sensing System for Biomedical Applications2020

    • Author(s)
      Sung Bae Kim, Sharon Seiko Hori, Negar Sadeghipour, Uday Kumar Sukumar, Rika Fujii, Tarik F. Massoud, and Ramasamy Paulmurugan
    • Journal Title

      Photochem Photobiol Sci

      Volume: 19 Issue: 4 Pages: 524-529

    • DOI

      10.1039/d0pp00017e

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Ligand-activated BRET9 imaging for measuring protein-protein interactions in living mice2020

    • Author(s)
      Sung Bae Kim, Rika Fujii, Arutselvan Natarajan, Tarik F. Massoud and
    • Journal Title

      Chem. Commun.

      Volume: 56 Issue: 2 Pages: 281-284

    • DOI

      10.1039/c9cc07634d

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] <i>In vitro</i> Determination of Rapamycin-triggered FKBP–FRB Interactions Using a Molecular Tension Probe2019

    • Author(s)
      KIM Sung Bae、NISHIHARA Ryo、FUJII Rika、PAULMURUGAN Ramasamy、CITTERIO Daniel、SUZUKI Koji
    • Journal Title

      Analytical Sciences

      Volume: 35 Issue: 1 Pages: 71-78

    • DOI

      10.2116/analsci.18SDP08

    • NAID

      130007554863

    • ISSN
      0910-6340, 1348-2246
    • Year and Date
      2019-01-10
    • Related Report
      2019 Annual Research Report 2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Biothiol-Activatable Bioluminescent Coelenterazine Derivative for Molecular Imaging in Vitro and in Vivo2019

    • Author(s)
      Nanako Nomura, Ryo Nishihara, Takahiro Nakajima, Takahiro Nakajima, Sung Bae Kim, Naoko Iwasawa, Yuki Hiruta, Shigeru Nishiyama, Moritoshi Sato, Daniel Citterio, Koji Suzuki
    • Journal Title

      Anal. Chem.

      Volume: 91(15) Issue: 15 Pages: 9546-9553

    • DOI

      10.1021/acs.analchem.9b00694

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Near Infrared bioluminescence imaging with Through‐Bond Energy Transfer Cassette2019

    • Author(s)
      Abe Masahiro、Nishihara Ryo、Ikeda Yuma、Nakajima Takahiro、Sato Moritoshi、Iwasawa Naoko、Nishiyama Shigeru、Paulmurugan Ramasamy、Citterio Daniel、Kim Sung Bae (CA)、Suzuki Koji (CA)
    • Journal Title

      ChemBioChem

      Volume: 20 Issue: 15 Pages: 1919-1923

    • DOI

      10.1002/cbic.201900149

    • Related Report
      2019 Annual Research Report 2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Molecular Imaging of Retinoic Acids in Live Cells Using Single-Chain Bioluminescence Probes2019

    • Author(s)
      Kim, Sung Bae; Fujii, Rika; Nishihara, Ryo; Bose, Rajendran J. C.; Citterio, Daniel; Suzuki, Koji; Massoud, Tarik F.; Paulmurugan, Ramasamy
    • Journal Title

      ACS Combinatorial Science

      Volume: 21(6) Issue: 6 Pages: 473-481

    • DOI

      10.1021/acscombsci.9b00035

    • Related Report
      2019 Annual Research Report 2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Highly bright and stable NIR-BRET with blue-shifted coelenterazine derivatives for deep-tissue imaging of molecular events in vivo2019

    • Author(s)
      Ryo Nishihara, Ramasamy Paulmurugan, Takahiro Nakajima, Eiji Yamamoto, Arutselvan Natarajan, Rayhaneh Afjei, Yuki Hiruta, Naoko Iwasawa, Shigeru Nishiyama, Daniel Citterio, Moritoshi Sato, Sung Bae Kim and Koji Suzuki
    • Journal Title

      Theranostics

      Volume: 9(9) Issue: 9 Pages: 2646-2661

    • DOI

      10.7150/thno.32219

    • Related Report
      2019 Annual Research Report 2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Azide- and Dye-Conjugated Coelenterazine Analogues for a Multiplex Molecular Imaging Platform2018

    • Author(s)
      Ryo Nishihara, Emi Hoshino, Yoshiki Kakudate, Satoshi Kishigami, Naoko Iwasawa, Shin-ichi Sasaki, Takahiro Nakajima, Moritoshi Sato, Shigeru Nishiyama, Daniel Citterio, Koji Suzuki, Sung Bae Kim
    • Journal Title

      Bioconjugate Chemistry (ACS)

      Volume: 印刷中 Issue: 6 Pages: 1922-1931

    • DOI

      10.1021/acs.bioconjchem.8b00188

    • Related Report
      2018 Research-status Report 2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] 化学物質による生理活性のイメージグ2018

    • Author(s)
      金 誠培、藤井 理香
    • Journal Title

      Pharm Tech Japan

      Volume: 34 Pages: 357-352

    • Related Report
      2017 Research-status Report
  • [Journal Article] Fabrication of a New Lineage of Artificial Luciferases from Natural Luciferase Pools2017

    • Author(s)
      Sung Bae Kim, Ryo Nishihara, Daniel Citterio, and Koji Suzuki
    • Journal Title

      ACS Combinatorial Science

      Volume: 19 Issue: 9 Pages: 594-599

    • DOI

      10.1021/acscombsci.7b00081

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Luciferase-Specific Coelenterazine Analogues for Optical Contamination-Free Bioassays2017

    • Author(s)
      Ryo Nishihara, Masahiro Abe, Shigeru Nishiyama, Daniel Citterio, Koji Suzuki, Sung Bae Kim
    • Journal Title

      Scientific Reports

      Volume: 印刷中 Issue: 1 Pages: 1-8

    • DOI

      10.1038/s41598-017-00955-6

    • Related Report
      2017 Research-status Report 2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Splitting-free Bioluminescence Imaging Probes and Their Applications2016

    • Author(s)
      Sung Bae Kim, Rika Fujii
    • Journal Title

      BUNSEKI KAGAKU

      Volume: 65 Issue: 7 Pages: 361-369

    • DOI

      10.2116/bunsekikagaku.65.361

    • NAID

      130005253342

    • ISSN
      0525-1931
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Fabrication of Molecular Tension Probes2016

    • Author(s)
      Sung Bae Kim, Rika Fujii
    • Journal Title

      MethodsX

      Volume: 3 Pages: 261-267

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] A genetically encoded bioluminescent indicator for illuminating proinflammatory cytokines2016

    • Author(s)
      Sung Bae Kim, Takeaki Ozawa, Yoshio Umezawa
    • Journal Title

      MethodsX

      Volume: 3 Pages: 483-489

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] How to Fabricate Functional Artificial Luciferases for Bioassays2016

    • Author(s)
      Sung Bae Kim, Rika Fujii
    • Journal Title

      Method in Molecular Biology

      Volume: 1461 Pages: 43-53

    • DOI

      10.1007/978-1-4939-3813-1_4

    • ISBN
      9781493938117, 9781493938131
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Single-chain probes for illuminating androgenicity of chemicals2016

    • Author(s)
      Sung Bae Kim, Hiroaki Tao
    • Journal Title

      Method in Molecular Biology

      Volume: 1461 Pages: 143-151

    • DOI

      10.1007/978-1-4939-3813-1_11

    • ISBN
      9781493938117, 9781493938131
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Multicolor Imaging of Bifacial Activities of Estrogens2016

    • Author(s)
      Sung Bae Kim, Yoshio Umezawa
    • Journal Title

      Method in Molecular Biology

      Volume: 1461 Pages: 153-163

    • DOI

      10.1007/978-1-4939-3813-1_12

    • ISBN
      9781493938117, 9781493938131
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Circular Permutation Probes for Illuminating Phosphorylation of Estrogen Receptor2016

    • Author(s)
      Sung Bae Kim, Hiroaki Tao
    • Journal Title

      Method in Molecular Biology

      Volume: 1461 Pages: 165-173

    • DOI

      10.1007/978-1-4939-3813-1_13

    • ISBN
      9781493938117, 9781493938131
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] A Genetically Encoded Molecular Tension Probe for Tracing Protein―Protein Interactions in Mammalian Cells2015

    • Author(s)
      Sung Bae Kim, Ryo Nishihara, Daniel Citterio, Koji Suzuki
    • Journal Title

      Bioconjugate Chemistry

      Volume: 27 (2) Issue: 2 Pages: 354-362

    • DOI

      10.1021/acs.bioconjchem.5b00421

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] Highly bright and stable NIR-BRET imaging systems for deep-tissue imaging of molecular events in vivo2019

    • Author(s)
      金 誠培・ 西原 諒 ・ 佐藤 守俊 ・ Citterio, Daniel ・ 鈴木 孝治 ・ Paulmurugan, Ramasamy
    • Organizer
      日本分析化学会、第68回年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Azide- and Dye-Conjugated Coelenterazine Analogues for a Multiplex Molecular Imaging Platform2019

    • Author(s)
      金 誠培 ・ 西原 諒 ・ Citterio, Daniel ・ 鈴木 孝治
    • Organizer
      日本分析化学会、第68回年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Near Infrared bioluminescence imaging with Through-Bond Energy Transfer Cassette2019

    • Author(s)
      金 誠培 ・ 阿部 将大 ・ 西原 諒 ・ Paulmurugan, Ramasamy ・ Citterio, Daniel ・ 鈴木 孝治
    • Organizer
      日本分析化学会、第68回年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Molecular imaging of retinoic acids in live cells using single-chain bioluminescence probes2019

    • Author(s)
      金 誠培 ・ 西原 諒 ・ Citterio, Daniel ・ 鈴木 孝治 ・ Paulmurugan, Ramasamy
    • Organizer
      日本分析化学会、第68回年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Ligand-Activated Reporter Protein Conformation for BRET Imaging of Protein-Protein Interactions in Living Mice2019

    • Author(s)
      金 誠培 ・ 藤井 理香 ・ Paulmurugan, Ramasamy
    • Organizer
      日本分析化学会、第68回年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] NIR-BRET imaging system for deep-tissue illumination of molecular events in vivo2019

    • Author(s)
      金 誠培・西原 諒・佐藤 守俊3・中島 隆浩・藤井 理香・Daniel Citterio・Ramasamy Paulmurugan・鈴木孝治
    • Organizer
      JABC 生物発光化学発光研究会 第35回学術講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Genetically encoded bioluminescent probes for imaging retinoic acids in live cells2019

    • Author(s)
      Sung Bae Kim・Rika Fujii・Ryo Nishihara・Daniel Citterio・Koji Suzuki・Ramasamy Paulmurugan
    • Organizer
      JABC 生物発光化学発光研究会 第35回学術講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Molecular Imaging of Immune Checkpoint Blockade in a Humanized Mouse Model of Triple Negative Breast Cancer Metastasis to Lungs using a New Deep Tissue Near-Infrared Bioluminescence Resonance Energy Transfer (BRET) system2018

    • Author(s)
      Ramasamy Paulmurugan、金 誠培
    • Organizer
      World Molecular Imaging Congress (WMIC) 2018
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] ホルモン様化学物質の生理活性評価技術の開発と応用2017

    • Author(s)
      金 誠培
    • Organizer
      InterAqua 2017
    • Place of Presentation
      East Hall 1, Tokyo Big Sight, Tokyo Japan
    • Related Report
      2016 Research-status Report
  • [Presentation] Fabrication of artificial luciferases and their applications to molecular imaging2016

    • Author(s)
      Sung Bae Kim
    • Organizer
      Royal Society of Chemistry (RSC) Tokyo International Conference 2016
    • Place of Presentation
      Makuhari Messe, Chiba (Japan)
    • Year and Date
      2016-09-08
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Book] Bioluminescence2016

    • Author(s)
      Kim, Sung-Bae (Ed.)
    • Publisher
      Springer-Humana Press
    • Related Report
      2016 Research-status Report
  • [Remarks] 人工生物発光酵素(ALuc&reg;)に選択的に発光する基質を開発

    • URL

      https://www.aist.go.jp/aist_j/new_research/2017/nr20171211/nr20171211.html

    • Related Report
      2018 Research-status Report
  • [Remarks] 人工生物発光酵素(ALuc&reg;)に選択的に発光する基質を開発

    • URL

      http://www.aist.go.jp/aist_j/new_research/2017/nr20171211/nr20171211.html

    • Related Report
      2017 Research-status Report
  • [Patent(Industrial Property Rights)] 生物発光酵素の長波長発光基質2020

    • Inventor(s)
      金誠培、牧昌次郎、北田昇雄
    • Industrial Property Rights Holder
      産業技術総合研究所
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2020-040703
    • Filing Date
      2020
    • Related Report
      2019 Annual Research Report
  • [Patent(Industrial Property Rights)] マルチチャンネル光検出装置2020

    • Inventor(s)
      金誠培
    • Industrial Property Rights Holder
      産業技術総合研究所
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2020-044513
    • Filing Date
      2020
    • Related Report
      2019 Annual Research Report
  • [Patent(Industrial Property Rights)] 新規セレンテラジン化合物及びその用途2018

    • Inventor(s)
      金誠培、鈴木孝治、チッテリオダニエル、西原諒、佐藤守俊
    • Industrial Property Rights Holder
      産業技術総合研究所
    • Industrial Property Rights Type
      特許
    • Filing Date
      2018
    • Related Report
      2018 Research-status Report
    • Overseas
  • [Patent(Industrial Property Rights)] 人工生物発光酵素に用いるための発光基質2018

    • Inventor(s)
      金誠培、和泉博、田尾博明、鳥村政基、脇坂昭弘
    • Industrial Property Rights Holder
      産業技術総合研究所
    • Industrial Property Rights Type
      特許
    • Filing Date
      2018
    • Acquisition Date
      2019
    • Related Report
      2018 Research-status Report
    • Overseas
  • [Patent(Industrial Property Rights)] 新規セレンテラジン化合物及びその用途2018

    • Inventor(s)
      金誠培、鈴木孝治、チッテリオダニエル、西原諒、佐藤守俊
    • Industrial Property Rights Holder
      産業技術総合研究所
    • Industrial Property Rights Type
      特許
    • Filing Date
      2018
    • Acquisition Date
      2019
    • Related Report
      2018 Research-status Report
    • Overseas
  • [Patent(Industrial Property Rights)] 人工生物発光酵素2017

    • Inventor(s)
      金誠培、鳥村政基、田尾博明、中里哲也
    • Industrial Property Rights Holder
      産業技術総合研究所
    • Industrial Property Rights Type
      特許
    • Filing Date
      2017
    • Acquisition Date
      2018
    • Related Report
      2018 Research-status Report
    • Overseas
  • [Patent(Industrial Property Rights)] 新規セレンテラジン化合物及びその用途2017

    • Inventor(s)
      鈴木, チッテリオ, 西原, 金 誠培, 佐藤, 中嶋
    • Industrial Property Rights Holder
      鈴木, チッテリオ, 西原, 金 誠培, 佐藤, 中嶋
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2017-055985
    • Filing Date
      2017-03-22
    • Related Report
      2016 Research-status Report
  • [Patent(Industrial Property Rights)] 新規人工生物発光酵素群2016

    • Inventor(s)
      金 誠培
    • Industrial Property Rights Holder
      金 誠培
    • Industrial Property Rights Type
      特許
    • Filing Date
      2016-09-30
    • Related Report
      2016 Research-status Report
    • Overseas

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Published: 2016-10-04   Modified: 2021-02-19  

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