| Project/Area Number |
15KK0190
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| Research Category |
Fund for the Promotion of Joint International Research (Fostering Joint International Research)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bio-related chemistry
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
UZAWA Takanori 国立研究開発法人理化学研究所, 創発物性科学研究センター, 専任研究員 (60554376)
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| Research Collaborator |
Hollfelder Florian University of Cambridge, Department of Biochemistry, Prof.
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| Project Period (FY) |
2016 – 2017
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000)
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| Keywords | 非天然アミノ酸 / アミノアシルtRNA合成酵素 / 進化分子工学 / 蛍光性アミノ酸、 |
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| Outline of Final Research Achievements |
As an extension form my primary subject that aimed to develop an in-cell signal amplification system using light as a trigger, I aimed to establish a system to introduce a fluorescence amino acid into a protein. First I established systems to screen aminoacyl-tRNA synthetases using E.coli and beads display that was acquired at University of Cambridge. Next, I confirmed that the system works using an unnatural amino acid that have been reported before. Then, using these systems, I obtained candidate mutants that would charge a fluorescent amino acid.
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