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Engineering of three-dimensional tissues using electrochemical approach(Fostering Joint International Research)

Research Project

Project/Area Number 15KK0232
Research Category

Fund for the Promotion of Joint International Research (Fostering Joint International Research)

Allocation TypeMulti-year Fund
Research Field Biofunction/Bioprocess
Research InstitutionYokohama National University

Principal Investigator

Fukuda Junji  横浜国立大学, 大学院工学研究院, 教授 (80431675)

Project Period (FY) 2016 – 2019
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000)
Keywords血管内皮細胞 / 肝細胞 / ティッシュエンジニアリング / 再生医療 / 三次元培養 / Tissue Engineering / Vascular network / Electrochemistry / Chemical engineering / 毛細血管 / 電気化学 / 光架橋性ゼラチン / 肝臓 / マイクロ流路 / 血管新生 / 電気化学細胞脱離 / 血管 / マイクロデバイス
Outline of Final Research Achievements

The lack of fabrication strategy of perfusable vascular networks is a fundamental barrier to engineer thick and cell-dense tissues and organs for regenerative medicine. Such fabrication processes should be sufficiently rapid to avoid severe hypoxic damage. We have proposed a rapid engineering approach of spatially-aligned and perfusable macrovasculatures for the supply of oxygen and nutrient throughout three-dimensional tissues. We designed an electrochemically cleavable oligopeptide which can be used for cell transfer from a culture surface to a hydrogel. Using this oligopeptide on a needle, macrovasculatures whose internal surface was covered with endothelial cells were fabricated in a hydrogel. Through this international collaboration project, we further applied this simple and versatile approach to engineer hepatic and pancreatic tissues in vitro.

Academic Significance and Societal Importance of the Research Achievements

細胞そのものを利用する新しい治療法として再生医療に期待が寄せられている。すでに皮膚や角膜などの薄い2次元的な組織は臨床応用が開始されており、その成果が示されている。しかし、肝臓や膵臓などの厚みのあるより重要な臓器・組織は、未だに作製が難しいのが現状である。これは、組織が厚くなると、酸素の供給が組織表面からの拡散のみでは不十分となるためである。つまり、cmサイズの立体的な組織の作製には、酸素や栄養素の運搬を担う血管網構造を作製する技術が必要であり、本研究プロジェクトでは、当該分野で独自の技術を有する海外の研究者と共同研究を実施し、この課題の解決に取り組んだ。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (40 results)

All 2020 2019 2018 2017 2016 Other

All Int'l Joint Research (4 results) Journal Article (9 results) (of which Int'l Joint Research: 7 results,  Peer Reviewed: 9 results,  Open Access: 6 results,  Acknowledgement Compliant: 1 results) Presentation (21 results) (of which Int'l Joint Research: 11 results,  Invited: 5 results) Book (3 results) Remarks (2 results) Patent(Industrial Property Rights) (1 results)

  • [Int'l Joint Research] Massachusetts Institute of Technology(米国)2018

    • Year and Date
      2018-08-06
    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Seoul National University(韓国)2018

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] University of Wollongong(オーストラリア)2018

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Politecnico di Milano, IRCCS Galeazzi Orthopaedic Institute(イタリア)2016

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Tailored cell sheet engineering using microstereolithography and electrochemical cell transfer2019

    • Author(s)
      Y. Kobayashi, C.E.J. Cordonier, Y. Noda, F. Nagase, J. Enomoto, T. Kageyama, H. Honma, S. Maruo, J. Fukuda
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 1802-1802

    • DOI

      10.1038/s41598-019-46801-9

    • NAID

      120006713342

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Preparation of hair beads and hair follicle germs for regenerative medicine2019

    • Author(s)
      T. Kageyama, L. Yan, A. Shimizu, S. Maruo, J. Fukuda
    • Journal Title

      Biomaterials

      Volume: 212 Pages: 55-63

    • DOI

      10.1016/j.biomaterials.2019.05.003

    • NAID

      120006707808

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Vascularized Bone-Mimetic Hydrogel Constructs by 3D Bioprinting to Promote Osteogenesis and Angiogenesis2019

    • Author(s)
      Anada Takahisa、Pan Chi-Chun、Stahl Alexander、Mori Satomi、Fukuda Junji、Suzuki Osamu、Yang Yunzhi
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 20 Issue: 5 Pages: 1096-1096

    • DOI

      10.3390/ijms20051096

    • Related Report
      2019 Annual Research Report 2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] ynergic effects of oxygen supply and antioxidants on pancreatic β-cell spheroids2019

    • Author(s)
      D. Myasnikova, T. Osaki, K. Onishi, T. Kageyama, B. Zhang, J. Fukuda
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 1802-1802

    • DOI

      10.1038/s41598-018-38011-6

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Engineering of perfusable double-layered vascular structures using contraction of spheroid-embedded hydrogel and electrochemical cell detachment2019

    • Author(s)
      Shimazu Yuka、Zhang Binbin、Yue Zhilian、Wallace Gordon G.、Fukuda Junji
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 127 Issue: 1 Pages: 114-120

    • DOI

      10.1016/j.jbiosc.2018.07.006

    • NAID

      120006539323

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Gold cleaning methods for preparation of cell culture surfaces for self-assembled monolayers of zwitterionic oligopeptides2018

    • Author(s)
      J. Enomoto, T. Kageyama, D. Myasnikova, K. Onishi, Y. Kobayashi, Y. Taruno, T. Kanai, J. Fukuda
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 125 Issue: 5 Pages: 606-612

    • DOI

      10.1016/j.jbiosc.2017.12.014

    • NAID

      40021552684

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Herringbone-like hydrodynamic structures in microchannels: A CFD model to evaluate the enhancement of surface binding2017

    • Author(s)
      E. Bianchi, M. Piergiovanni, C. Arrigoni, J. Fukuda, A. Gautieri, M. Moretti, G. Dubini
    • Journal Title

      Medical Engineering and Physics

      Volume: 48 Pages: 62-67

    • DOI

      10.1016/j.medengphy.2017.07.003

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Catch-and-Release of Target Cells Using Aptamer-Conjugated Electroactive Zwitterionic Oligopeptide SAM2017

    • Author(s)
      J. Enomoto, T. Kageyama, T. Osaki, F. Bonalumi, F. Marchese, A. Gautieri, E. Bianchi, G. Dubini, C. Arrigoni, M. Moretti, and J. Fukuda
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 43375-43375

    • DOI

      10.1038/srep43375

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Rational Design of Prevascularized Large 3D Tissue Constructs Using Computational Simulations and Biofabrication of Geometrically Controlled Microvessels2016

    • Author(s)
      C. Arrigoni, M. Bongio, G. Dubini, S. Bersini, J. Enomoto, J. Fukuda, M. Moretti
    • Journal Title

      Advanced Healthcare Materials

      Volume: 5 Issue: 13 Pages: 1617-26

    • DOI

      10.1002/adhm.201500958

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] ハイドロゲルの変形を利用した四次元組織構築法の確立2019

    • Author(s)
      小澤聖奈、景山達斗、Binbin Zhang、福田淳二
    • Organizer
      日本動物細胞工学会 2019
    • Related Report
      2019 Annual Research Report
  • [Presentation] 細胞形態モニタリング装置とこれを用いた細胞分化能の予測2019

    • Author(s)
      増本美波、大崎達哉、景山達斗、Binbin Zhang、木村博之、坂根勲、高橋晋太郎、瀧本真一、福田淳二
    • Organizer
      日本動物細胞工学会 2019
    • Related Report
      2019 Annual Research Report
  • [Presentation] 毛髪再生医療のための細胞培養技術2019

    • Author(s)
      福田淳二
    • Organizer
      日本バイオマテリアル学会第9回九州ブロック講演会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 細胞形態モニタリング装置と細胞分化機能の予測2019

    • Author(s)
      増本美波、大崎達哉、景山達斗、Binbin Zhang、木村博之、坂根勲、高橋晋太郎、瀧本真一、福田淳二
    • Organizer
      日本動物実験代替法学会第32回
    • Related Report
      2019 Annual Research Report
  • [Presentation] 4D tissue engineering using hydrogel swelling and shrinkage2019

    • Author(s)
      Sena Ozawa, Binbin Zhang, Junji Fukuda
    • Organizer
      APCChE2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Cell Morphology Monitoring during Culture for Daily Cell Quality Control2019

    • Author(s)
      Minami Masumoto, Tatsuya Osaki, Tatsuto Kageyama, Binbin Zhang, Isao Sakane, Shintaro Takahashi, Shinichi Takimoto & Junji Fukuda
    • Organizer
      TERMIS-AM 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Antioxidant and Glucose Influence on ROS Production and Insulin Secretion of Pancreatic β-cell Spheroids Cultured in Polydimethylsiloxane Microwell Array Device2019

    • Author(s)
      Dina Myasnikova, Junji Fukuda
    • Organizer
      TERMIS-AP 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Vascularized bone grafts prepared with hydrogel micro-dispenser and 3D mesh printing2019

    • Author(s)
      Hikaru Akieda, Tatsuto Kageyama, Yohei Noda, Shoji Maruo, Junji Fukuda
    • Organizer
      2019 MRS fall meeting & exihibit
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Engineering 3D tissues in vitro2019

    • Author(s)
      Junji Fukuda
    • Organizer
      2019 Ischemic/hypoxic Disease Institute International Symposium
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 血管を含む立体組織をつくるアプローチ2018

    • Author(s)
      福田淳二
    • Organizer
      第80回日本生物工学会大会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] 神経・血管相互作用を利用したダブルネットワークの形成誘導2018

    • Author(s)
      林慎也、大崎達哉、福田淳二
    • Organizer
      日本動物実験代替法学会 第31回大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 電気化学細胞脱離のための金表面洗浄法2018

    • Author(s)
      楯芳樹、榎本詢子、景山達斗、福田淳二、松下淳、秋本紗希、根本悠平
    • Organizer
      化学工学会 第84年会
    • Related Report
      2018 Research-status Report
  • [Presentation] Engineering small and large tissues based on oxygen supply2018

    • Author(s)
      Junji Fukuda
    • Organizer
      TERMIS-WC 2018
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Fabrication of vascularized bone grafts using bone beads2018

    • Author(s)
      Hikaru Akieda, Yukie Sonoyama, Tatsuto Kageyama, Yohei Noda, Shoji Maruo, Junji Fukuda
    • Organizer
      TERMIS-WC 2018
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 送液可能な血管様構造を用いた血管新生評価デバイスの開発2017

    • Author(s)
      篠原礼奈、榎本詢子、大﨑達哉、小関智光、中尾裕利、福田淳二
    • Organizer
      第16回日本再生医療学会総会
    • Place of Presentation
      仙台国際センター
    • Year and Date
      2017-03-07
    • Related Report
      2016 Research-status Report
  • [Presentation] Surface Design for Catch-and-release of Target Cells using Electrochemical Cell Detachment2017

    • Author(s)
      Junko Enomoto, AlfonsoGautieri, Junji Fukuda
    • Organizer
      12th Annual International Electromaterials Science Symposium
    • Place of Presentation
      North Wollongong, Australia
    • Year and Date
      2017-02-08
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Engineering 3D tissues using oxygen-permeable spheroid plate2016

    • Author(s)
      Kisaki Onishi, Junko Enomoto, Rena Shinohara, Dina Myasnikova, Junji Fukud
    • Organizer
      Termis-AM 2016
    • Place of Presentation
      San diego, USA
    • Year and Date
      2016-12-11
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Array of perfusable three-dimensional microvasculatures on active tilting stage2016

    • Author(s)
      Rena Shinohara, Junko Enomoto, Tatsuya Osaki, Yuka Kobayashi, Tomomitsu Ozeki, Hirotoshi Nakao, Junji Fukuda
    • Organizer
      2016MRS Fall Meeting
    • Place of Presentation
      Boston, USA
    • Year and Date
      2016-11-27
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Transplantation of tailored cell sheets using micro stereolithography and electrochemical cell transfer2016

    • Author(s)
      Yuka Kobayashi, Christopher E.J. Cordonier, Fuminori Nagase, Hideo Honma, Shoji Maruo, Junji Fukuda
    • Organizer
      International Conference on Biofabrication 2016
    • Place of Presentation
      Winston-Salem, USA
    • Year and Date
      2016-10-29
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] アプタマー修飾オリゴペプチド層を用いた選択的な細胞のキャッチ&リリース2016

    • Author(s)
      榎本詢子、Alfonso Gautieri、福田淳二
    • Organizer
      第68回日本生物工学会大会(2016)
    • Place of Presentation
      富山国際会議場
    • Year and Date
      2016-09-28
    • Related Report
      2016 Research-status Report
  • [Presentation] マイクロ光造形法と生体適合性金めっきを用いた立体細胞シートの作製2016

    • Author(s)
      小林優香、Christopher E.J. Cordonier、永瀬史憲、本間英夫、丸尾昭二、福田淳二
    • Organizer
      第68回日本生物工学会大会(2016)
    • Place of Presentation
      富山国際会議場
    • Year and Date
      2016-09-28
    • Related Report
      2016 Research-status Report
  • [Book] 生体適合無電解金めっきを用いたテーラーメイド型細胞シート移植法2020

    • Author(s)
      Christopher E.J. Cordonier、福田淳二
    • Publisher
      日刊工業新聞社
    • Related Report
      2019 Annual Research Report
  • [Book] 血管を有する組織を作るアプローチ、骨格筋研究を核とした筋スマート社会2019

    • Author(s)
      林慎也、景山達斗、大崎達哉、福田淳二
    • Publisher
      シー・エム・シー出版
    • ISBN
      9784904482629
    • Related Report
      2019 Annual Research Report
  • [Book] 生体適合性無電解金めっきの研究2019

    • Author(s)
      Christopher E.J. Cordonier、鈴木陽平、福田淳二、丸尾昭二、本間英夫
    • Publisher
      (一社)表面技術協会
    • Related Report
      2019 Annual Research Report
  • [Remarks] Tailored cell sheets to improve post-operative..

    • URL

      https://www.eurekalert.org/pub_releases/2019-09/ynu-ts091819.php

    • Related Report
      2019 Annual Research Report
  • [Remarks] 'テーラーメイド'の3次元構造細胞シートの作成に成功

    • URL

      https://eurekalert.org/pub_releases_ml/2019-09/ynu-4091819.php

    • Related Report
      2019 Annual Research Report
  • [Patent(Industrial Property Rights)] 培養装置及び培養方法2016

    • Inventor(s)
      小関智光、中尾裕利、福田淳二、篠原礼奈、大崎達哉
    • Industrial Property Rights Holder
      株式会社アルバック、横浜国立大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2016-04-28
    • Related Report
      2016 Research-status Report

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Published: 2016-10-04   Modified: 2021-02-19  

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