Structural study of a huge respiratory supermolecule hemocyanin (international collaboration work)(Fostering Joint International Research)
Project/Area Number |
15KK0248
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Research Category |
Fund for the Promotion of Joint International Research (Fostering Joint International Research)
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Allocation Type | Multi-year Fund |
Research Field |
Structural biochemistry
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Research Institution | Tohoku University (2017-2018) Hokkaido University (2015-2016) |
Principal Investigator |
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Research Collaborator |
Raunser Stefan Max Planck Institute of Molecular Physiology, ディレクター
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Project Period (FY) |
2015 – 2018
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Project Status |
Completed (Fiscal Year 2018)
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Budget Amount *help |
¥13,520,000 (Direct Cost: ¥10,400,000、Indirect Cost: ¥3,120,000)
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Keywords | ヘモシアニン / 立体構造 / クライオ電顕 / 対称性 / クライオ電子顕微鏡 / X線結晶構造解析 / 構造解析 / タンパク質 |
Outline of Final Research Achievements |
Hemocyanin is an oxygen transporter of mollusks. Squid hemocyanin is a huge cylindrical protein complex with a molecular mass approximately 4 MDa. In a previous study, we determined crystal structure of squid hemocyanin at 3.0 angstrom resolution. However, due to the crystal packing and symmetry of the outer wall region, we could not determine precise structure of the inside domains. Therefore, in the present study, we used cryo-electrom microscopy single particle analysis method to determine the structure. The revealed strcutre showed that the inner domains are arranged asymmetrically. As well, insight into molecular evolution of hemocyanin was revealed.
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Academic Significance and Societal Importance of the Research Achievements |
X線結晶構造解析では結晶中の全ての分子の平均構造が得られるため,外壁領域と内部領域の対称性が異なる場合,分子同士の接触に影響を与える外壁領域の影響を受けやすい.先行研究にてイカヘモシアニンのX線結晶構造解析を行った際も,D5対称性を持つ外壁領域の影響を受け,内部の構造もD5の対称性で平均化され,その正確な構造を決定できなかった.本研究では,外部領域と内部領域を個別に精密化することができるクライオ電子顕微鏡単粒子解析法を用いることにより,ヘモシアニンの非対称な内部構造を決定することができた.本研究により,対称性がミスマッチした分子の構造解析におけるクライオ電子顕微鏡解析の有用性が示された.
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Report
(5 results)
Research Products
(29 results)
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[Journal Article] Cryo-EM reveals the asymmetric assembly of squid hemocyanin2019
Author(s)
Tanaka, Y., Kato, S., Stabrin, M., Raunser, S., Matsui, T. & Gatsogiannis, C.
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Journal Title
International Union of Crystallography (IUCr)
Volume: 6
Issue: 3
Pages: 426-437
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Encapsulation of biomacromolecules by soaking and co-crystallization into porous protein crystals of hemocyanin2019
Author(s)
Tsubasa Hashimoto, Yuxin Ye, Asuka Matsuno, Yuki Ohnishi, Akira Kitamura, Masataka Kinjo, Satoshi Abe, Takafumi Uenod, Min Yao, Tomohisa Ogawa, Takashi Matsuia, Yoshikazu Tanaka
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Journal Title
Biochemical and Biophysical Research Communications
Volume: 509
Issue: 2
Pages: 577-584
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Compact seahorse-shaped T cell-activating antibody for cancer therapy2018
Author(s)
Hiroto Fujii, Yoshikazu Tanaka, Hikaru Nakazawa, Aruto Sugiyama, Noriyoshi Manabe, Akira Shinoda, Nobutaka Shimizu, Takamitsu Hattori, Katsuhiro Hosokawa, Takuma Sujino, Tomoyuki Ito, Teppei Niide, Ryutaro Asano, Izumi Kumagai, and Mitsuo Umetsu
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Journal Title
Advanced Therapeutics
Volume: 1700031
Issue: 3
Pages: 1-10
DOI
Related Report
Peer Reviewed
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[Journal Article] Structural basis for tRNA-dependent cysteine biosynthesis2017
Author(s)
Chen Meirong、Kato Koji、Kubo Yume、Tanaka Yoshikazu、Liu Yuchen、Long Feng、Whitman William B.、Lill Pascal、Gatsogiannis Christos、Raunser Stefan、Shimizu Nobutaka、Shinoda Akira、Nakamura Akiyoshi、Tanaka Isao、Yao Min
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Journal Title
Nature Commun.
Volume: 8
Issue: 1
Pages: 1521-1521
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Biochemical and structural characterization of oxygen-sensitive 2-thiouridine synthesis catalyzed by the iron-sulfur protein TtuA2017
Author(s)
Minghao Chen, Shin-ichi Asai, Shun Narai, Shusuke Nambu, Naoki Omura, Yuriko Sakaguchi, Tsutomu Suzuki, Masao Ikeda-Saito, Kimitsuna Watanabe, Min Yao, Naoki Shigi and, Yoshikazu Tanaka
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Journal Title
Proc. Natl. Acad. Sci. USA
Volume: 印刷中
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Presentation] 巨大タンパク質会合体ヘモシアニンの多孔質性結晶を用いた生体分子の包摂2016
Author(s)
Asuka Matsuno, Ye Yuxin, Yuki Ohnishi, Akira Kitamura, Masataka Kinjo, Satoshi Abe, Takafumi Ueno, Yoshikazu Tanaka, Min Yao
Organizer
第54回日本生物物理学会年会
Place of Presentation
つくば国際会議場(茨城県,つくば市)
Year and Date
2016-11-25
Related Report
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