| Outline of Final Research Achievements |
Pyruvate kinase (PK) catalyzes the last irreversible step of glycolysis, and is known to be one of the rate-limiting enzyme of glycometabolism. There are four PK isoforms in mammals, PKM2 knockout lead to the reduction of cancer cell growth. Therefore, to elucidate the molecular mechanism of PKM2 enzymatic activity by atomic level is very valuable to develop a new drug on various kinds of cancer. In this project, we use the latest cryo-electron microscopy machine to resolve the atomic model of human PKM2 wild type and patient-derived mutant PKM2 H391Y. By using a single particle analysis, we clarified PKM2-WT, and PKM2-H391Y for the resolution of 2.9 Å and 3.1 Å, respectively. Taken together, we learned how to use a breakthrough technology in structural biology field in this international collaborating grant.
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