| Project/Area Number |
15KK0263
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| Research Category |
Fund for the Promotion of Joint International Research (Fostering Joint International Research)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurophysiology / General neuroscience
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Shimogori Tomomi 国立研究開発法人理化学研究所, 脳科学総合研究センター, チームリーダー (30391981)
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| Research Collaborator |
Gan Wenbiao Skirball Institute of Biomolecular Medicine, Molecular Neurobiology・Department of Physiology and Neuroscience, Professor
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| Project Period (FY) |
2016 – 2017
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥15,600,000 (Direct Cost: ¥12,000,000、Indirect Cost: ¥3,600,000)
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| Keywords | イメージング / 樹状突起 / 多光子 / ２光子イメージング / 発達 / 神経回路 / 分子メカニズム / in vivo イメージング / 生後発達 |
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| Outline of Final Research Achievements |
To reveal dynamic dendritic morphology change that is controlled by early life experience, we tried to develop two photon imaging technique on early postnatal mouse brain. The most difficult step for this experiment is (1) to keep animal survive without mother (artificial raring), (2) remove skull without damaging brain, (3) develop all tools that fits to small mouse head. Collaboration with Dr. Wenbiao Gan (NYU), who is a world expert of two photon imaging, we developed all procedure for early postnatal mouse brain imaging. First, we developed the most efficient procedure for artificial raring (feed goat milk) which kept the animal developing as normal rate. Next we discovered how to remove thin skull and also developed the device fits for small animal head for imaging. As a result, we succeeded to image single neuron from postnatal (P) day 2 to P5 and revealed dynamic dendritic morphology change.
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