Dedifferentiation to redifferentiation: Molecular mechanism on de novo shoot formation at wound site(Fostering Joint International Research)

• Project/Area Number: 15KK0265
• Research Category: Fund for the Promotion of Joint International Research (Fostering Joint International Research)
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Iwase Akira 国立研究開発法人理化学研究所, 環境資源科学研究センター, 研究員 (40553764)
• Research Collaborator: Roudier François École normale supérieure, Institut de Biologie de l'École Normale Supérieure, 教授
• Project Period (FY): 2016 – 2018
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥15,600,000 (Direct Cost: ¥12,000,000、Indirect Cost: ¥3,600,000)
• Keywords: 細胞リプログラミング / 脱分化 / 再分化 / カルス形成 / 転写因子 / 傷害応答 / 分化全能性 / ストレス / エピジェネティクス / ヒストン修飾 / ストレス応答 / 組織培養 / エピゲノム

Outline of Final Research Achievements

Plants have remarkable capacity for regeneration upon stresses. However, molecular mechanisms how wounding induces callus formation and successive organ/individual regeneration are still elusive. Recent studies start to uncover that epigenome alteration is critical steps for gene expression and cell fate specification. This study, by comprehensive understanding of these factors, succeeded to reveal a part of mechanisms on plant cell dedifferentiation and redifferentiation.

Academic Significance and Societal Importance of the Research Achievements

細胞の分化や脱分化は時空間的に連続した様々な遺伝子発現の結果であり、近年の生物学の重要な成果から、エピゲノムの変化が遺伝子発現制御に重要な役割を担うことが明らかになってきた。これらの変化と植物の分化・脱分化についての研究分野はここ数年のうちに盛んになってきている。本研究によって、植物細胞の脱分化・再分化時のエピゲノム変化をいち早く網羅的に理解出来たことは、現象の基礎的理解を深める基盤として今後も学術的な貢献をすると考えられる。また、得られた知見は、組織培養技術の改良にも応用でき、社会的な広がりが期待できる。

Research Products

• [Int'l Joint Research] 国立科学研究センター（CNRS）(フランス) (2016)
• [Journal Article] WIND1 induces dynamic metabolomic reprogramming during regeneration in Brassica napus (2018)
  • Author(s): Akira Iwase, Kento Mita, David S.Favero, Nobutaka Mitsuda, Ryosuke Sasaki, Makoto Kobayshi, Yumiko Takebayashi, Mikiko Kojima, Miyako Kusano, Akira Oikawa, Hitoshi Sakakibara, Kazuki Saito, Jun Imamura, Keiko Sugimoto
  • Journal Title: Developmental Biology Volume: 442 Issue: 1 Pages: 40-52
  • DOI: 10.1016/j.ydbio.2018.07.006
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Wounding Triggers Callus Formation via Dynamic Hormonal and Transcriptional Changes (2017)
  • Author(s): Ikeuchi M, Iwase A, Rymen B, Lambolez A, Kojima M, Takebayashi Y, Heyman J, Watanabe S, Seo M, De Veylder L, Sakakibara H, Sugimoto K
  • Journal Title: Plant Physiology Volume: 175 Issue: 3 Pages: 1158-1174
  • DOI: 10.1104/pp.17.01035
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] ABA Suppresses Root Hair Growth via the OBP4 Transcriptional Regulator. (2017)
  • Author(s): Rymen, B., Kawamura, A., Schafer, S., Breuer, C., Iwase, A., Shibata, M., Ikeda, M., Mitsuda, N., Koncz, C., Ohme-Takagi, M., Matsui, M., and Sugimoto, K.
  • Journal Title: Plant physiology Volume: 173 Issue: 3 Pages: 1750-1762
  • DOI: 10.1104/pp.16.01945
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] WIND1 promotes shoot regeneration through transcriptional activation of ESR1 in Arabidopsis. (2017)
  • Author(s): 1.Iwase A, Harashima H, Ikeuchi M, Rymen B, Ohnuma M, Komaki S, Morohashi K, Kurata T, Nakata M, Ohme-Takagi M, Grotewold E, Sugimoto K
  • Journal Title: Plant Cell Volume: 1 Issue: 1 Pages: 54-69
  • DOI: 10.1105/tpc.16.00623
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] Wound-induced molecular network leading to regeneration (2019)
  • Author(s): Akira Iwase, Momoko Ikeuchi, Bart Rymen, Alice Lambolez, Takamasa Suzuki, Nobutaka Mitsuda, Keiko Sugimoto
  • Organizer: International Symposium: Principles of pluripotent stem cells underlying plant vitality
  • Int'l Joint Research / Invited
• [Presentation] Stress-induced plant cell reprogramming: Accelerators and brakes for regeneration (2017)
  • Author(s): Akira Iwase
  • Organizer: Seminar at Royal Holloway University of London
  • Place of Presentation: Royal Holloway University of London (Egham, UK)
  • Year and Date: 2017-03-22
  • Int'l Joint Research / Invited
• [Presentation] Stress-induced plant cell reprogramming: Accelerators and brakes for regeneration (2017)
  • Author(s): Akira Iwase
  • Organizer: Seminar at ENS-Lyon
  • Place of Presentation: ENS-Lyon (Lyon, France)
  • Year and Date: 2017-03-20
  • Int'l Joint Research / Invited
• [Presentation] Stress-induced plant cell reprogramming: Accelerators and brakes for regeneration (2017)
  • Author(s): Akira Iwase
  • Organizer: Max Planck Institute Special Seminar
  • Place of Presentation: Max Planck Institute (Cologne, Germany)
  • Year and Date: 2017-02-24
  • Int'l Joint Research / Invited
• [Presentation] WIND1転写因子の下流分子ネットワーク解析 (2017)
  • Author(s): 岩瀬 哲、光田展隆、池内桃子、鈴木孝征、杉本慶子
  • Organizer: 日本植物学会 第81回大会
• [Presentation] Stress-induced plant cell reprogramming: Accelerators and brakes for regeneration (2017)
  • Author(s): Akira Iwase
  • Organizer: Seminar at Institut Jean-Pierre Bourgin, INRA Versailles-Grignon
• [Presentation] Wound-induced cellular reprogramming in Arabidopsis (2017)
  • Author(s): Akira Iwase, Bart Rymen, Momoko Ikeuchi, Hui Lan, Marie Pireyre, Takamasa Suzuki, Nobutaka Mitsuda , Katja Jaeger and Keiko Sugimoto
  • Organizer: Bilateral Closure Symposium of GDRI Integrative Plant Biology Network "The developing plant in its environment"
• [Presentation] WIND1-induced cellular reprogramming in Arabidopsis (2016)
  • Author(s): Akira Iwase, Nobutaka Mitsuda, Kengo Morohashi, Takamasa Suzuki, Hirofumi Harashima, Momoko Ikeuchi, Bart Rymen, Erich Grotewold and Keiko Sugimoto
  • Organizer: Cold Spring Harbor Asia, Latest Advances in Plant Development & Environmental Response
  • Place of Presentation: 淡路夢舞台国際会議場（淡路市、兵庫県）
  • Year and Date: 2016-12-01
  • Int'l Joint Research
• [Presentation] ストレスで誘起される細胞リプログラミングの分子機構 (2016)
  • Author(s): 岩瀬哲
  • Organizer: 日本植物学会第80回大会シンポジウム Induced Development：環境要因に誘発される発生の多様性と共通性
  • Place of Presentation: 沖縄コンベンションセンター(宜野湾市、沖縄県）
  • Year and Date: 2016-09-16
  • Invited
• [Remarks] 植物が傷口で茎葉を再生させる仕組み －組織培養による植物の量産や有用物質生産に期待－
  • URL: http://www.riken.jp/pr/press/2017/20170117_2/
• [Remarks] 環境記憶統合“注目の論文” 傷口から蘇る ～ カルス形成から茎葉再分化への分子カスケード ～
  • URL: http://www.rs.tus.ac.jp/plantmemory/research-achievement/paper/no11.html
• [Remarks] Wound-induced shoot regeneration pathway
  • URL: http://www.csrs.riken.jp/en/press/press170117-2.html
• [Remarks] How plants shoot back
  • URL: http://www.riken.jp/en/research/rikenresearch/highlights/8342/