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Mechanism of antibacterial effect of eseential amino acid(Fostering Joint International Research)

Research Project

Project/Area Number 15KK0267
Research Category

Fund for the Promotion of Joint International Research (Fostering Joint International Research)

Allocation TypeMulti-year Fund
Research Field Food science
Research InstitutionHokkaido University

Principal Investigator

Shigenobu Koseki  北海道大学, 農学研究院, 准教授 (70414498)

Research Collaborator Ross Tom  University of Tasmania, Agricultural Science, Professor
Koutsoumanis Kostas  Aristotle University of Thessaloniki, Department of Food Science and Technology, Professor
Project Period (FY) 2016 – 2018
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Keywords適合溶質 / トリプトファン / 増殖抑制 / 予測微生物学 / アミノ酸 / 予測モデル / Single cell / 殺菌 / 確率論
Outline of Final Research Achievements

The aim of the present study was to elucidate the mode of action of D-Trp as an antibacterial agent. We investigated two different hypotheses: D-Trp is incorporated into bacteria and acts intracellularly, or D-Trp acts outside the bacterial cell and inhibits membrane function. E. coli incorporated D-Trp added to the culture medium regardless of the subsequent growth effect. Similar amounts of E. coli metabolites were detected when incubated under the growth-promoting condition of 3% NaCl and the growth-suppression condition of 3% NaCl with 40 mM D-Trp. By contrast, guanosine 3′,5′-bisdiphosphate was only detected under the growth-suppression condition. However, once E. coli cells with D-Trp-induced growth suppression were transferred to a proliferative environment, proliferation was only suppressed in the environment with specific extracellular concentrations of NaCl and D-Trp. We conclude that D-Trp influences membrane function to exert its antimicrobial effects.

Academic Significance and Societal Importance of the Research Achievements

D-トリプトファンの利用が新規の微生物制御手法として世界的な認知度が向上し,世界中の人々がより安全で高品質な食品を届けることに貢献する。また,基礎研究面においても,細菌のストレス応答に関する新たな評価法,知見が蓄積され,今後の微生物制御ならびにストレス応答メカニズムの解明に寄与することが期待される。実用面においても実際の生牡蠣の保存流通中における腸炎ビブリオの増殖を顕著に抑制できることを見出したことから,生牡蠣のみならず多様な生鮮海産物品の微生物学的な安全性を確保した流通体系の確立に大いに貢献することが期待できる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (9 results)

All 2018 2017

All Int'l Joint Research (2 results) Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (3 results) (of which Int'l Joint Research: 3 results)

  • [Int'l Joint Research] テッサロニキ・アリストテレス大学(ギリシャ)2018

    • Year and Date
      2018-05-20
    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] タスマニア大学(オーストラリア)2017

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Growth Inhibitory Effect of d-tryptophan on Vibrio spp. in Shucked and Live Oysters2018

    • Author(s)
      Chen J, Kudo H, Kan K, Kawamura S, Koseki S.
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 84 Issue: 19

    • DOI

      10.1128/aem.01543-18

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characteristics of d-Tryptophan as an Antibacterial Agent: Effect of Sodium Chloride Concentration and Temperature on Escherichia coli Growth Inhibition2018

    • Author(s)
      Kaito Kan, Jian Chen, Shuso Kawamura, and Shigenobu Koseki
    • Journal Title

      Journal of Food Protection

      Volume: 81 Issue: 1 Pages: 25-30

    • DOI

      10.4315/0362-028x.jfp-17-229

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Estimation of the probability of bacterial population survival: Development of a probability model to describe the variability in time to inactivation of Salmonella enterica2017

    • Author(s)
      Kento Koyama, Hidekazu Hokunan, Mayumi Hasegawa, Shuso Kawamura, Shigenobu Koseki
    • Journal Title

      Food Microbiology

      Volume: 68 Pages: 121-128

    • DOI

      10.1016/j.fm.2017.07.007

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Modeling Stochastic Variability in the Numbers of Surviving Salmonella enterica, Enterohemorrhagic Escherichia coli, and Listeria monocytogenes Cells at the Single-Cell Level in a Desiccated Environment2017

    • Author(s)
      Kento Koyama, Hidekazu Hokunan, Mayumi Hasegawa, Shuso Kawamura, Shigenobu Koseki
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 83 Issue: 4

    • DOI

      10.1128/aem.02974-16

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Presentation] Growth Inhibitory Effect of D-tryptophan on Vibrio spp. in Broth culture, Seawater and live Oysters2018

    • Author(s)
      Jian Chen and Shigenobu Koseki
    • Organizer
      Annual meeting, International Association for Food Protection
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Antibacterial activity of D-tryptophan against Salmonella enterica and Escherichia coli O157:H7 under osmotic stress and its application to oysters2017

    • Author(s)
      Jian Chen and Shigenobu Koseki
    • Organizer
      Annual meeting, International Association for Food Protection
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Describing time-to-inactivation of bacterial population, using individual cell heterogeneity via computer simulation2017

    • Author(s)
      Kento Koyama, Hiroki Abe Shuso Kawamura, Shigenobu Koseki
    • Organizer
      10th International Conference of Predictive Modelling in Food (ICPMF10)
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research

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Published: 2016-10-04   Modified: 2020-03-30  

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