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Isolation of genes expressed asymmetrically in the flounder metamorphosis and analysis of regulatory mechanisms by thyroid hormone(Fostering Joint International Research)

Research Project

Project/Area Number 15KK0272
Research Category

Fund for the Promotion of Joint International Research (Fostering Joint International Research)

Allocation TypeMulti-year Fund
Research Field Aquatic life science
Research InstitutionTohoku University

Principal Investigator

Yokoi Hayato  東北大学, 農学研究科, 助教 (40569729)

Project Period (FY) 2016 – 2019
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Keywordsヒラメ / 異体類 / 変態 / 左右非対称化 / 色素胞分化 / トランスクリプトーム / ヒラメ・カレイ類 / 水産増養殖 / ゼブラフィッシュ / 左右非対称 / 色素胞 / 大西洋イシビラメ / 次世代シーケンサー / 水産学 / ゲノム / 左右非対称性
Outline of Final Research Achievements

Flatfish transform their body plan during metamorphosis. Flatfish are important fish as commercial target and actively produced by aquaculture, however, the abnormalities in pigmentation and malformation are the common issue around the world. In this international collaboration, we investigated the metamorphosis of flatfish comparing Japanese flounder and Mediterranean turbot. New findings include, blind-side specific degeneration of cartilage around eye was specific phenomena for flounder and not observed in turbot; genes involved in pigmentation is conserved among teleost fish but not in several tetrapods.

Academic Significance and Societal Importance of the Research Achievements

ヒラメなど水産上重要な魚種では活発に増養殖がおこなわれているが、無眼側の黒化など体色異常や形態の異常が問題である。これらの問題は海外の増養殖においても共通の問題であり、日本産のヒラメとヨーロッパのターボットを比較解析することにより、現象の普遍性と種特異性および進化的な側面について考察をすることができた。また共通の問題に取り組むことで共同研究として連携が生まれ、EUの国際共同研究プログラムにも参画することができた。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (57 results)

All 2020 2019 2018 2017 2016 Other

All Int'l Joint Research (3 results) Journal Article (8 results) (of which Peer Reviewed: 8 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (44 results) (of which Int'l Joint Research: 16 results,  Invited: 5 results) Book (1 results) Remarks (1 results)

  • [Int'l Joint Research] ハイデルベルグ大学(ドイツ)2016

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] バルセロナ大学(スペイン)2016

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] 国立海洋研究所(スペイン)2016

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Developmental regulatory system of ocular‐side‐specific asymmetric pigmentation in flounder: Critical role of retinoic acid signaling.2020

    • Author(s)
      Qiran Chen, Kota Sato, Hayato Yokoi, Tohru Suzuki
    • Journal Title

      Journal of Experimental Zoology-B Molecular and Developmental Evolution

      Volume: 334 Issue: 3 Pages: 156-167

    • DOI

      10.1002/jez.b.22934

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Plasmid delivery by electroporation into fish skeletal muscle for recombinant protein secretion and uptake by oocytes2020

    • Author(s)
      岩泉雅樹 横井勇人 鈴木徹
    • Journal Title

      Fish Physiology and Biochemistry

      Volume: 2020 Issue: 3 Pages: 1121-1130

    • DOI

      10.1007/s10695-020-00775-y

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mother-to-embryo vitellogenin transport in a viviparous teleost Xenotoca eiseni2019

    • Author(s)
      Iida Atsuo、Arai Hiroyuki N.、Someya Yumiko、Inokuchi Mayu、Onuma Takeshi A.、Yokoi Hayato、Suzuki Tohru、Hondo Eiichi、Sano Kaori
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 116 Issue: 44 Pages: 22359-22365

    • DOI

      10.1073/pnas.1913012116

    • NAID

      120006731188

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Expression profiles of RA synthases and catabolic enzymes in newly hatched and metamorphosing larvae of Japanese flounder, Paralichthys olivaceus2018

    • Author(s)
      Chen Qiran、Yokoi Hayato、Suzuki Tohru
    • Journal Title

      General and Comparative Endocrinology

      Volume: 269 Pages: 60-67

    • DOI

      10.1016/j.ygcen.2018.08.015

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Identification of Sox10-positive cells at the dorsal fin base of juvenile flounder that are correlated with blind-side skin ectopic pigmentation2018

    • Author(s)
      Togawa Mai、Endo Yuna、Suzuki Nobuo、Yokoi Hayato、Suzuki Tohru
    • Journal Title

      Journal of Experimental Zoology Part B: Molecular and Developmental Evolution

      Volume: 330 Issue: 8 Pages: 427-437

    • DOI

      10.1002/jez.b.22842

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Analyses of the cellular clock gene expression in peripheral tissue, caudal fin, in the Japanese flounder, Paralichthys olivaceus2017

    • Author(s)
      M. Mogi, S. Yokoi, T. Suzuki,
    • Journal Title

      General and Comparative Endocrinology

      Volume: 印刷中 Pages: 97-105

    • DOI

      10.1016/j.ygcen.2017.02.009

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] External Asymmetry and Pectoral Fin Loss in the Bamboo Sole (Heteromycteris japonica): Small-Sized Sole with Potential as a Pleuronectiformes Experimental Model2017

    • Author(s)
      Chen Q, Takagi M, Mogi M, Kikuchi M, Saito Y, Nakamura S, Yokoi H, Seikai T, Uji S and Suzuki T
    • Journal Title

      Zoolog Sci

      Volume: 34 Issue: 5 Pages: 377-385

    • DOI

      10.2108/zs170021

    • NAID

      40021336139

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Excess retinoic acid induces fusion of centra by degenerating intervertebral ligament cells in Japanese flounder, Paralichthys olivaceus2017

    • Author(s)
      Wu X, Chen Q, Washio Y, Yokoi H, Suzuki T
    • Journal Title

      Journal of Experimental Zoology, Part B, Molecular and Developmental Evolution

      Volume: 326B Issue: 8 Pages: 464-473

    • DOI

      10.1002/jez.b.22717

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 魚類の色素胞分化に関与する遺伝子dhfr-likeおよびhddの解析2020

    • Author(s)
      中村峻也, 安齋賢, 成瀬清, 鈴木徹, 横井勇人
    • Organizer
      日本水産学会春季大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 免疫組織化学によるヒラメ仔魚および稚魚におけるグルココルチコイド受容体(GcR; Nr3c1)の局在観察2020

    • Author(s)
      Naomi Hadisumarto, 常田真葵音, 横井勇人, 鈴木徹
    • Organizer
      日本水産学会春季大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] エレクトロポーレーションを用いたヒラメ稚魚内の Sox10 陽性色素前駆細胞の in vivo での可視化2020

    • Author(s)
      三宅みなと, 鈴木徹, 横井勇人
    • Organizer
      日本水産学会春季大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ササウシノシタ仔魚における Sox10 陽性 - 色素前駆細胞の分布とWntアンタゴニストの影響2020

    • Author(s)
      佐藤航大, 宇治督, 横井勇人, 鈴木徹
    • Organizer
      日本水産学会春季大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Critical role of RA signaling during the asymmetric pigmentation of metamorphosing Japanese flounder2020

    • Author(s)
      陳其然, 佐藤航大, 横井勇人, 鈴木徹
    • Organizer
      日本水産学会春季大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 雌親魚の腹腔内注射による卵母細胞へのタンパク質の導入2020

    • Author(s)
      岩泉雅樹, 横井勇人, 宇治督, 鈴木徹
    • Organizer
      日本水産学会春季大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] RNA-seqで明らかになった小型異体類、ササウシノシタの仔魚発生・変態期における遺伝子発現プロファイルの変化2019

    • Author(s)
      齋藤雄大, 横井勇人, 宇治督, 鈴木徹
    • Organizer
      日本動物学会 第90回 大阪大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Characterization of medaka dhfr-like and hdd, genes involved in pigment cell differentiation2019

    • Author(s)
      Hayato Yokoi, Shunya Nakamura, Satoshi Ansai, Kiyoshi Naruse and Tohru Suzuki
    • Organizer
      25th Japanese Medaka and Zebrafish Meeting
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Immunohistochemical localization of glucocorticoid receptor (Nr3c1) in the brain of Japanese flounder (Paralichthys olivaceus) larva and juvenile2019

    • Author(s)
      Naomi Hadisumarto, Makine Tsuneta, Hayato Yokoi, Tohru Suzuki
    • Organizer
      25th Japanese Medaka and Zebrafish Meeting
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ゲノム編集による養殖有用系統作出の効率化に向けた技術展開研究2019

    • Author(s)
      岩泉雅樹, 横井勇人, 鈴木徹
    • Organizer
      日本ゲノム編集学会 第4回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] dhfr and hdd, genes involved in pigment cell differentiation isolated by transcriptome analysis of flounder metamorphosis2019

    • Author(s)
      Hayato Yokoi, Shunya Nakamura, Satoshi Ansai, Kiyoshi Naruse, Tohru Suzuki
    • Organizer
      52nd Annual Meeting of the Japanese Society of Developmental Biologists, Co-sponsored by APDBN
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Distribution of Sox10-positive chromatophore progenitors and neurons in metamorphosing flounder larvae2019

    • Author(s)
      Tohru Suzuki, Minato Miyake, Kota Sato, Hayato Yokoi
    • Organizer
      52nd Annual Meeting of the Japanese Society of Developmental Biologists, Co-sponsored by APDBN
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ヒラメの着色型黒化進行に伴う脳と皮膚のtranscriptomeの変化2019

    • Author(s)
      齋藤雄大、篠原怜、松田直往、田川正朋、横井勇人、鈴木徹
    • Organizer
      日本水産学会春季大会
    • Related Report
      2018 Research-status Report
  • [Presentation] RNA-Seq screening of up- and down-regulated genes by retinoic acid in metamorphosing flounder larvae2019

    • Author(s)
      陳其然、横井勇人、鈴木徹
    • Organizer
      日本水産学会春季大会
    • Related Report
      2018 Research-status Report
  • [Presentation] トランスクリプトーム解析により明らかになったササウシノシタの変態に伴う遺伝子発現プロファイルの変化2019

    • Author(s)
      齋藤雄大、横井勇人、宇治督、鈴木徹
    • Organizer
      日本水産学会春季大会
    • Related Report
      2018 Research-status Report
  • [Presentation] エレクトロポレーションを用いた魚類への遺伝子導入効果の検討2019

    • Author(s)
      岩泉雅樹、関根通陽、横井勇人、鈴木徹
    • Organizer
      日本水産学会春季大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 色素胞分化に関与する新規遺伝子の解析2019

    • Author(s)
      中村峻也、安齋賢、成瀬清、鈴木徹、横井勇人
    • Organizer
      日本水産学会春季大会
    • Related Report
      2018 Research-status Report
  • [Presentation] Genes involved in pigment cell differentiation isolated by gene expression profiling of flounder metamorphosis2018

    • Author(s)
      Hayato Yokoi, Shunya Nakamura, Laura Cal, Josep Rotllant, Tohru Suzuki
    • Organizer
      24th Japanese Medaka and Zebrafish Meeting
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] The bamboo sole (Heteromycteris japonica): A small-sized flatfish suitable as a laboratory model for metamorphic development in the Pleuroneciformes2018

    • Author(s)
      Tohru Suzuki, Chen Qiran, Hayato Yokoi
    • Organizer
      24th Japanese Medaka and Zebrafish Meeting
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] ササウシノシタ(Heteromycteris japonica)仔魚における鰭神経回路の発生と色素前駆細胞の局在2018

    • Author(s)
      菊池美希、齋藤雄大、遠藤優女、陳其然、横井勇人、宇治督、鈴木徹
    • Organizer
      日本動物学会第89回札幌大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 変態期ヒラメの RNA-seq 解析で単離された色素胞分化に関与する遺伝子の解析2018

    • Author(s)
      横井勇人、Rotllant Josep、Cal Laura、鈴木徹
    • Organizer
      日本水産学会春季大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 魚類における視交叉上核を介した体内時計の制御機構に関する研究2018

    • Author(s)
      茂木淳、横井勇人、鈴木徹
    • Organizer
      日本水産学会春季大会
    • Related Report
      2017 Research-status Report
  • [Presentation] ヒラメの着色型黒化部に現れる黒色素胞の起源について2018

    • Author(s)
      十川麻衣、松田直往、田川正朋、横井勇人、鈴木徹
    • Organizer
      日本水産学会春季大会
    • Related Report
      2017 Research-status Report
  • [Presentation] ヒラメ下垂体における時計遺伝子と内分泌関連遺伝子の概日リズムに関する解析2018

    • Author(s)
      丹羽祥太、茂木淳、横井勇人、鈴木徹
    • Organizer
      日本水産学会春季大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 給餌条件により生じるヒラメ体色異常の遺伝子発現プロファイリング解析2017

    • Author(s)
      國政実里、横井勇人、酒井義文、青海忠久、鈴木徹
    • Organizer
      日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京都・港区)
    • Year and Date
      2017-03-26
    • Related Report
      2016 Research-status Report
  • [Presentation] ヒラメ再生皮膚に現れる色素前駆細胞と色素胞の分布2017

    • Author(s)
      十川麻衣、茂木淳、横井勇人、鈴木徹
    • Organizer
      日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京都・港区)
    • Year and Date
      2017-03-26
    • Related Report
      2016 Research-status Report
  • [Presentation] Expression profiling of Japanese flounder abnormal pigmentation caused by feeding conditions2017

    • Author(s)
      Kunimasa M, ○Yokoi H, Sakai Y, Seikai T and Susuki T
    • Organizer
      50th annual meeting for the Japanese Society of Developmental Biologists (JSDB) cosponsored by the Asia-Pacific Developmental Biology Network (APDBN)
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Small-scale culture system of bamboo sole (Heteromycteris japonica), a small-sized species of the Pleuroneciformes, and its metamorphic external asymmetry and pectoral fin loss2017

    • Author(s)
      Suzuki T, Chen Q, Uji S and Yokoi H
    • Organizer
      50th annual meeting for the Japanese Society of Developmental Biologists (JSDB) cosponsored by the Asia-Pacific Developmental Biology Network (APDBN)
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] RHYTHMIC PER2 EXPRESSION AT THE SUPRACHIASMATIC NUCLEUS OF THE JAPANESE FLOUNDER, PARALICHYTHYS OLIVACEUS, AND ITS IMPLICATIONS FOR CIRCADIAN CLOCK MECHANISM2017

    • Author(s)
      Suzuki T, Mogi M and Yokoi H
    • Organizer
      18th International Congress of Comparative Endocrinology (ICCE18)
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] EXPRESSION OF THREE CLOCK GENES, PER1, PER2 AND CRY1 IN THE CAUDAL FIN OF JAPANESE FLOUNDER2017

    • Author(s)
      Mogi M, Yokoi H and Suzuki T
    • Organizer
      18th International Congress of Comparative Endocrinology (ICCE18)
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Transcriptome analysis of flatfish metamorphosis identified ocular side biased genes involved in pigmentation2017

    • Author(s)
      Yokoi H, Kunimasa M, Sakai Y and Suzuki T
    • Organizer
      10th European Zebrafish Meeting
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Whole genome duplication and diversification of gene function in teleosts: lessons from Fgfr1, Sox9 and Aldh genes2017

    • Author(s)
      Yokoi H, Qiran C, Canestro C, Postlethwait JH and Suzuki T
    • Organizer
      XI Congress of Iberian Association for Comparative Endocrinology (AIEC)
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] ヒラメ変態期の左右非対称な形態形成と細胞分化2017

    • Author(s)
      横井勇人、鈴木徹
    • Organizer
      第3回ユニークな少数派実験動物を扱う若手が最先端アプローチを勉強する会
    • Related Report
      2017 Research-status Report
  • [Presentation] Expression profile of RA-related genes during metamorphic development in Japanese flounder2017

    • Author(s)
      Chen Q, Yokoi H and Suzuki T
    • Organizer
      23rd Japanese Medaka and Zebrafish Meeting
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Application of electroporation to zebrafish and bubble-eye goldfish for exogenous protein synthesis in the ovary and skeletal muscle2017

    • Author(s)
      Iwaizumi M, Yokoi H and Suzuki T
    • Organizer
      23rd Japanese Medaka and Zebrafish Meeting
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] ヒラメの無眼側皮膚に異所的に出現する色素胞の由来について2017

    • Author(s)
      十川 麻衣、茂木淳、横井勇人、鈴木徹
    • Organizer
      日本動物学会 第88回 富山大会
    • Related Report
      2017 Research-status Report
  • [Presentation] トラフグの脊椎骨で椎間板様組織を構成する脊索のトランスクリプトーム解析2017

    • Author(s)
      鈴木徹、明珍大志、横井勇人
    • Organizer
      日本動物学会 第88回 富山大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Pigmentation genes isolated by transcriptome expression profiling of flounder metamorphosis2017

    • Author(s)
      Yokoi H, Cal L, Rotllant J and Susuki T
    • Organizer
      2017年度生命科学系学会合同年次大会、第40回日本分子生物学会年会、第90回日本生化学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Expression profiling of flatfish, a weird teleost fish that transforms into an asymmetric body plan2016

    • Author(s)
      Yokoi H, Kunimasa M, Washio Y, Wu X, Sakai Y Seikai T and Suzuki T
    • Organizer
      The joint meeting of the 22nd International Congress of Zoology and the 87th Meeting of the Zoological Society of Japan
    • Place of Presentation
      Okinawa Convention Center (Okinawa, Ginowan)
    • Year and Date
      2016-11-14
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 次世代シーケンサーを用いた遺伝子発現プロファイリングによるヒラメの体色異常に関与する遺伝子の解析2016

    • Author(s)
      國政実里、横井勇人、青海忠久、鈴木徹
    • Organizer
      日本水産学会北海道-東北合同支部大会
    • Place of Presentation
      北海道大学水産科学研究院(北海道・函館市)
    • Year and Date
      2016-10-22
    • Related Report
      2016 Research-status Report
  • [Presentation] Expression of RA synthases, aldh1a1, 1a2 and 1a3, in newly hatched, metamorphic larvae and adult of Japanese founder2016

    • Author(s)
      陳其然、横井勇人、鈴木徹
    • Organizer
      日本水産学会秋季大会
    • Place of Presentation
      近畿大学農学部(奈良県・奈良市)
    • Year and Date
      2016-09-08
    • Related Report
      2016 Research-status Report
  • [Presentation] ブラジル産とユタ産アルテミアを与えたヒラメ変態期仔魚で発現量の異なる遺伝子のRNA-Seqによるスクリーニング2016

    • Author(s)
      國政実里、青海忠久、鈴木徹、横井勇人
    • Organizer
      日本水産学会秋季大会
    • Place of Presentation
      近畿大学農学部(奈良県・奈良市)
    • Year and Date
      2016-09-08
    • Related Report
      2016 Research-status Report
  • [Presentation] ヒラメ:左右非対称な眼の移動と色素胞分化2016

    • Author(s)
      横井勇人
    • Organizer
      第2回ユニークな少数派実験動物を扱う若手が最先端アプローチを勉強する会
    • Place of Presentation
      岡崎コンファレンスセンター(愛知県・岡崎市)
    • Year and Date
      2016-08-21
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] Transcriptome analysis of flounder metamorphosis2016

    • Author(s)
      Yokoi H, Kunimasa M, Sakai Y and Suzuki T
    • Organizer
      22nd Japanese medaka and zebrafish meeting
    • Place of Presentation
      Okazaki Conference Center (Aichi, Okazaki)
    • Year and Date
      2016-08-20
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Book] Whole genome duplication and diversification of gene function in teleosts: lessons from Fgfr1, Sox9 and Aldh genes. In "Advances in Comparative Endocrinology, vol IX".2017

    • Author(s)
      Yokoi H, Chen Q, Canestro C, Postlethwait JH, Suzuki T
    • Publisher
      Universida de Vigo
    • ISBN
      9788481587586
    • Related Report
      2017 Research-status Report
  • [Remarks] 東北大学研究者紹介

    • URL

      http://db.tohoku.ac.jp/whois/detail/0b42e27653b7f35e08dfd12bc8aceb84.html

    • Related Report
      2017 Research-status Report

URL: 

Published: 2016-10-04   Modified: 2021-02-19  

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