| Outline of Final Research Achievements |
In this study, we identified UBX domain-containing protein 1 (UBXN1), a negative regulator of RIG-I-like receptor signaling, as a host protein that interacts with NiV V. Further, we focused on NiV-C trafficking in cells and found that it localizes predominantly in the cytoplasm but partly in the nucleus, and determined that amino acids 60-75 and 72-75 were important for nuclear localization of NiV-C. In the study of role of the accessory proteins in swine cells, it was shown that NiV protein prevents induction of type I IFN and is important for production of infectious virus. We developed new recombinant vaccine for swine NiV infection. We constructed attenuated PRV virus expressing NiV G protein, and performed safety and efficacy tests in Canadian BSL4. The results showed that the virus preparation in vaccinated pigs apparently suppressed compared with unvaccinated pigs.
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