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Characterization of a novel symbiosis pathway in legume-rhizobium symbiosis(Fostering Joint International Research)

Research Project

Project/Area Number 15KK0276
Research Category

Fund for the Promotion of Joint International Research (Fostering Joint International Research)

Allocation TypeMulti-year Fund
Research Field Plant nutrition/Soil science
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

OKAZAKI SHIN  東京農工大学, (連合)農学研究科(研究院), 准教授 (40379285)

Research Collaborator Giraud Eric  フランス農業開発研究国際協力センター, 地中海熱帯地域共生研究室, グループ長
Gottfert Michael  
Project Period (FY) 2016 – 2018
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥12,480,000 (Direct Cost: ¥9,600,000、Indirect Cost: ¥2,880,000)
Keywords根粒菌 / マメ科植物 / III型分泌系 / エフェクター / 共生 / 根粒形成 / 3型分泌系 / 根粒 / Aeschynomene / クサネム / 窒素固定 / 生物間相互作用
Outline of Final Research Achievements

Rhizobium-derived Nod factors (NFs) have been long believed to be essential for legume - rhizobium symbiosis. Bradyrhizobium sp. STM6978, however, establish symbiosis with legumes in the NF-independent manner. In this study, we first identified a secreted protein ET-Nod in STM6978 which can induce nodulation in the NF-independent manner. Besides, when expressed in host root cells, ET-Nod could induce the development of nodule-like structures. These results indicated that ET-Nod is responsible for activating the NF-independent nodulation signaling. This is the first finding that rhizobial factor except for the NF can trigger the leguminous nodulation signaling.

Academic Significance and Societal Importance of the Research Achievements

マメ科植物と根粒菌の共生には根粒菌が作るNod factor (NF)が必須であると長い間考えられていた。本研究の成果は、この教科書的概念を覆し、根粒菌の分泌タンパク質(ET-Nod)が単独でマメ科植物の根粒形成シグナルを起動できることを示した初めての事例である。
今後ET-Nodの宿主植物側標的や宿主細胞内での生化学的機能、NFシグナリングとの相違点を解明することで、
マメ科植物と根粒菌の共進化を紐解く重要な知見となることが期待される。また、本エフェクターを利用することで、将来的にはイネやコムギなどNF受容機構のない非マメ科植物への根粒形成に貢献することが期待される。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (19 results)

All 2018 2017 2016 Other

All Int'l Joint Research (1 results) Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 4 results) Presentation (12 results) (of which Int'l Joint Research: 7 results,  Invited: 3 results) Remarks (2 results)

  • [Int'l Joint Research] フランス農業開発研究国際協力センター(フランス)2016

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Effects of Different Sources of Nitrogen on Endophytic Colonization of Rice Plants by <i>Azospirillum</i> sp. B5102018

    • Author(s)
      Naher K, Miwa H, Okazaki S, Yasuda M
    • Journal Title

      Microbes and Environments

      Volume: 33 Issue: 3 Pages: 301-308

    • DOI

      10.1264/jsme2.ME17186

    • NAID

      130007493355

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] InnB, a novel type III effector of Bradyrhizobium elkanii USDA61, controls symbiosis with Vigna Species2018

    • Author(s)
      Nguyen HP, Ratu STN, Yasuda M, Gottfert M, Okazaki S
    • Journal Title

      Frontiers in Microbiology

      Volume: 9 Pages: 1-10

    • DOI

      10.3389/fmicb.2018.03155

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of Bradyrhizobium elkanii Genes Involved in Incompatibility with Vigna radiata2017

    • Author(s)
      Nguyen Hien、Miwa Hiroki、Kaneko Takakazu、Sato Shusei、Okazaki Shin
    • Journal Title

      Genes

      Volume: 8 Issue: 12 Pages: 374-374

    • DOI

      10.3390/genes8120374

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] How effectors promote beneficial interactions2017

    • Author(s)
      Miwa Hiroki、Okazaki Shin
    • Journal Title

      Current Opinion in Plant Biology

      Volume: 38 Pages: 148-154

    • DOI

      10.1016/j.pbi.2017.05.011

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] A novel T3 effector of Bradyrhizobium elkanii plays a dual function in symbiosis with legumes2018

    • Author(s)
      Hien P Nguyen、Safirah T. N. Ratu、Wint Y. T. Phoo、Michiko Yasuda、 Shin Okazaki
    • Organizer
      JSME annual meeting & 10th ASME
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Infection mechanism of Bradyrhizobium elkanii by type III secretion system2018

    • Author(s)
      Michiko Yasuda、Shin Okazaki
    • Organizer
      JSME annual meeting & 10th ASME
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] マメ科植物との共生を司る根粒菌のIII型分泌系2017

    • Author(s)
      岡崎伸
    • Organizer
      平成29年度植物感染生理談話会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] Symbiotic roles of rhizobial type III effectors2017

    • Author(s)
      Shin Okazaki
    • Organizer
      20th International Congress on Nitrogen Fixation
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Molecular mechanisms underlying Vigna radiata-rhizobia interactions2017

    • Author(s)
      Nguyen PH, Okazaki S
    • Organizer
      20th International Congress on Nitrogen Fixation
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] A novel type III effector of Bradyrhizobium elkanii abolishing infection and nodule development in Vigna radiate2017

    • Author(s)
      Nguyen HP, Okazaki S.
    • Organizer
      植物微生物研究会第27回研究交流会
    • Related Report
      2017 Research-status Report
  • [Presentation] Rj4 ダイズ非親和性を決定するダイズ根粒菌 3 型分泌装置エフェクター2016

    • Author(s)
      増田幸子,三輪大樹,安田美智子,Omar, M. Faruque,岡崎伸
    • Organizer
      植物微生物研究会第26回研究交流会
    • Place of Presentation
      片平キャンパスさくらホール(宮城県仙台市)
    • Year and Date
      2016-09-07
    • Related Report
      2016 Research-status Report
  • [Presentation] 根粒菌のエフェクターによるミヤコグサ根粒形成の制御2016

    • Author(s)
      三輪大樹,安田美智子,増田幸子,金子貴一,佐藤修正,岡崎伸
    • Organizer
      植物微生物研究会第26回研究交流会
    • Place of Presentation
      片平キャンパスさくらホール(宮城県仙台市)
    • Year and Date
      2016-09-07
    • Related Report
      2016 Research-status Report
  • [Presentation] 根粒菌エフェクター誘導型免疫による共生非親和性の決定機構2016

    • Author(s)
      安田美智子,三輪大樹,増田幸子,竹林裕美子,榊原均,岡崎伸
    • Organizer
      植物微生物研究会第26回研究交流会
    • Place of Presentation
      片平キャンパスさくらホール(宮城県仙台市)
    • Year and Date
      2016-09-07
    • Related Report
      2016 Research-status Report
  • [Presentation] Symbiotic roles of the type III secretion system in Bradyrhizobium elkanii2016

    • Author(s)
      Shin Okazaki, Hiroki Miwa, Michiko Yasuda, Sachiko Masuda
    • Organizer
      第12回ヨーロッパ窒素固定会議
    • Place of Presentation
      ハンガリー、ブタペスト
    • Year and Date
      2016-08-25
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Rhizobial type III effector protein regulates soybean nodulation2016

    • Author(s)
      Sachiko Masuda, Hiroki Miwa, Michiko Yasuda, Omar M. Faruqueand Shin Okazaki
    • Organizer
      第12回ヨーロッパ窒素固定会議
    • Place of Presentation
      ハンガリー、ブタペスト
    • Year and Date
      2016-08-25
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Rhizobial type III secretion system and symbiosis with legumes2016

    • Author(s)
      Okazaki Shin
    • Organizer
      第17回国際植物微生物分子相互作用学会
    • Place of Presentation
      米国、オレゴン州ポートランド
    • Year and Date
      2016-07-17
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Remarks] 植物微生物研究室

    • URL

      http://web.tuat.ac.jp/~okazaki/index.html

    • Related Report
      2018 Annual Research Report
  • [Remarks] 植物微生物研究室 (岡崎研究室)

    • URL

      http://web.tuat.ac.jp/~okazaki/

    • Related Report
      2017 Research-status Report

URL: 

Published: 2016-10-04   Modified: 2020-03-30  

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