The actual host range of plasmids in microbial consortia(Fostering Joint International Research)

• Project/Area Number: 15KK0278
• Research Category: Fund for the Promotion of Joint International Research (Fostering Joint International Research)
• Allocation Type: Multi-year Fund
• Research Field: Applied microbiology
• Research Institution: Shizuoka University
• Principal Investigator: Shintani Masaki 静岡大学, 工学部, 准教授 (20572647)
• Research Collaborator: Wagner Michael ウイーン大学, 微生物学部, 教授 ; Smalla Kornelia
• Project Period (FY): 2016 – 2018
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
• Keywords: プラスミド / 宿主 / 嫌気性細菌 / 複合微生物系 / 接合伝達 / FISH法 / プラスミドキャプチャリング

Outline of Final Research Achievements

By exogenous plasmid capturing, many self-transmissible plasmids were obtained from microbial community in natural environments including IncP-1 and PromA group plasmids. Some of plasmids in PromA group showed differences in their GC contents around 10%. Although their gene sets were similar, their host range were different with each other. Conjugation assays under aerobic and anaerobic conditions showed that concentration of oxygens could change the kinds of transconjugants of IncP-1 plasmids.

Academic Significance and Societal Importance of the Research Achievements

本研究の成果は，自然界で遺伝子の水平伝播を実際に担っているプラスミドに関するこれまでの知見は，まだ不足していることを示している．こうしたプラスミドの探索を続けると共に，それらの宿主域を明らかにすることで，微生物の進化・適応機構を促すプラスミドの伝播経路の一端が明らかになると期待される．また，薬剤耐性遺伝子の伝播が各国で深刻な問題を引き起こしているが，本現象にもプラスミドの接合伝達機構が深く関与しており，本成果はこうした分野の研究者にも重要な知見を与える．

Research Products

• [Int'l Joint Research] ウィーン大学(オーストリア) (2017)
• [Journal Article] High-resolution comparison of bacterial conjugation frequencies (2019)
  • Author(s): Shintani Masaki, Ohkuma Moriya, Kimbara Kazuhide
  • Journal Title: Journal of Visualized Experiments Volume: - Issue: 143
  • DOI: 10.3791/57812
  • Peer Reviewed
• [Journal Article] Plasmids and Their Hosts (2019)
  • Author(s): Shintani Masaki, Suzuki Haruo
  • Journal Title: DNA Traffic in the Environment (H. Nishida, T. Oshima (eds.)), Springer Volume: - Pages: 109-133
  • DOI: 10.1007/978-981-13-3411-5_6
  • ISBN: 9789811334108, 9789811334115
  • Peer Reviewed
• [Journal Article] Novel Self-Transmissible and Broad-Host-Range Plasmids Exogenously Captured From Anaerobic Granules or Cow Manure (2018)
  • Author(s): Yanagiya Kosuke、Maejima Yoshiaki、Nakata Hiroki、Tokuda Maho、Moriuchi Ryota、Dohra Hideo、Inoue Kengo、Ohkuma Moriya、Kimbara Kazuhide、Shintani Masaki
  • Journal Title: Frontiers in Microbiology Volume: 9 Pages: 2602-2602
  • DOI: 10.3389/fmicb.2018.02602
  • Peer Reviewed / Open Access
• [Journal Article] The behavior of mobile genetic elements (MGEs) in different environments (2017)
  • Author(s): Masaki Shintani
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 81 Issue: 5 Pages: 854-862
  • DOI: 10.1080/09168451.2016.1270743
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 新規プラスミドグループPromA群に属するGC含量の異なるプラスミドの宿主域比較 (2019)
  • Author(s): 德田真穂，柳谷洸輔，井上謙吾，雪真弘，大熊盛也，水口千穂，野尻秀昭，金原和秀，新谷政己
  • Organizer: 日本農芸化学会2019年度大会
• [Presentation] 好気・微好気・嫌気条件下におけるプラスミドの接合伝達性の比較 (2018)
  • Author(s): 越智健太郎，柳谷洸輔，德田真穂，大熊盛也，金原和秀，新谷政己
  • Organizer: 日本農芸化学会2019年度大会
• [Presentation] 環境試料からの新規自己伝達性プラスミドの取得と解析 (2018)
  • Author(s): 早川雅也，前島由明，金原和秀，新谷政己
  • Organizer: 日本農芸化学会2019年度大会
• [Presentation] Behaviors of plasmids in microbial consortia under aerobic and anaerobic conditions (2018)
  • Author(s): Shintani M
  • Organizer: Special Talk in Chulalongkorn University
  • Int'l Joint Research / Invited
• [Presentation] 好気・微好気・嫌気条件下におけるプラスミドの接合伝達性の比較 (2018)
  • Author(s): 越智健太郎, 柳谷洸輔, 大熊盛也, 金原和秀, 新谷政己
  • Organizer: 第70回日本生物工学会大会
  • Int'l Joint Research
• [Presentation] Collecting and functional analysis of novel self-transmissible plasmid from environmental samples (2018)
  • Author(s): Maejima Y，Nakata H, Moriuchi R, Dohra H, Inoue K, Kimbara K，Shintani M，
  • Organizer: Plasmid Biology 2018
  • Int'l Joint Research
• [Presentation] PromA group plasmids isolated from environmental samples (2018)
  • Author(s): Maejima Y，Yanagiya K, Nakata H, Moriuchi R, Dohra H, Inoue K, Kimbara K，Shintani M
  • Organizer: Plasmid Biology 2018
  • Int'l Joint Research
• [Presentation] Comparisons of transferability and host range of conjugative plasmids under aerobic, microaerobic, and anaerobic conditions (2018)
  • Author(s): Yanagiya K, Inoue K, Minakuchi C, Nojiri H, Ohkuma M, Kimbara K, Shintani M
  • Organizer: Plasmid Biology 2018
  • Int'l Joint Research
• [Presentation] Behaviors of conjugative plasmids in different environmental conditions (2018)
  • Author(s): Shitnani M
  • Organizer: International Symposium for Microbial Ecology 17
  • Int'l Joint Research / Invited
• [Presentation] Behaviors of conjugative plasmids in different environmental conditions (2018)
  • Author(s): Shintani M
  • Organizer: JKI mini-symposium “Networking and cooperation in microbial ecology”
  • Int'l Joint Research / Invited
• [Presentation] 新規宿主・ベクター系の構築を目指した自己伝達性プラスミドの取得と機能解析 (2018)
  • Author(s): 前島由明，仲田裕貴，森内良太，道羅英夫，井上謙吾，金原和秀，新谷政己
  • Organizer: 日本農芸化学会2018年度大会
• [Presentation] 好気・微好気・嫌気条件下におけるプラスミドの接合伝達性と宿主域の解析 (2018)
  • Author(s): 柳谷洸輔，越智健太郎，仲田裕貴，井上謙吾，水口千穂，野尻秀昭，大熊盛也，金原和秀，新谷政己
  • Organizer: 日本農芸化学会2018年度大会
• [Presentation] 好気・微好気・嫌気条件下におけるプラスミドの接合伝達性と宿主域の解析 (2017)
  • Author(s): 越智健太郎，柳谷洸輔，井上謙吾，水口千穂，野尻秀昭，大熊盛也，金原和秀，新谷政己
  • Organizer: 日本農芸化学会中部支部 第180回 例会
• [Presentation] 微好気・嫌気条件下におけるプラスミドの接合伝達性と宿主域の解析 (2017)
  • Author(s): 柳谷洸輔，井上謙吾，野尻秀昭，大熊盛也，金原和秀，新谷政己
  • Organizer: 環境微生物系学会合同大会2017
• [Presentation] Transferability of plasmids in various environmental conditions (2017)
  • Author(s): 新谷政己
  • Organizer: 奈良先端科学技術大学院大学セミナー
  • Invited
• [Presentation] Behaviors of plasmids in soil microcosms (2017)
  • Author(s): Shintani M, Nour EH, Elsayed T, Blau K, Bziuk N, Jechalke S, Sproer C, Bunk B, Overmann J, Smalla K
  • Organizer: 6th International Symposium on Biosorption and Biodegradation/Bioremediation
  • Int'l Joint Research / Invited
• [Presentation] Transferability of plasmids in various environmental conditions (2017)
  • Author(s): 新谷政己
  • Organizer: Thailand-Japan Collaboration Symposium On Environmental Microbiology and Applications
  • Place of Presentation: Chulalongkorn University（タイ王国・バンコク）
  • Int'l Joint Research / Invited
• [Presentation] 異なる環境試料からの新規自己伝達性プラスミドの取得 (2016)
  • Author(s): 仲田裕貴，金原和秀，新谷政己
  • Organizer: 第68会日本生物工学会
  • Place of Presentation: 富山国際会議場（富山県，富山市）
  • Year and Date: 2016-09-28
• [Presentation] 微好気・嫌気環境下におけるプラスミドの宿主域の解析 (2016)
  • Author(s): 柳谷洸輔，片岡大亮，金原和秀，大熊盛也，新谷政己
  • Organizer: 日本農芸化学会第177回中部支部例会
  • Place of Presentation: 名古屋大学（愛知県，名古屋市）
• [Presentation] 環境細菌間における可動性遺伝因子の挙動に関する研究 (2016)
  • Author(s): 新谷政己
  • Organizer: 日本農芸化学会第176回中部支部例会
  • Place of Presentation: 岐阜大学（岐阜県，岐阜市）
  • Invited
• [Presentation] Behaviors of plasmids and their hosts in different environments (2016)
  • Author(s): 新谷政己
  • Organizer: Wissenshaftlishes Kolloquium, Julius Kuehn-Institut
  • Place of Presentation: Julius Kuehn-Institut（ドイツ・ブラウンシュバイク）
  • Int'l Joint Research / Invited
• [Remarks] 金原・新谷研究室
  • URL: https://kimbara-shintani.eng.shizuoka.ac.jp/
• [Remarks] 静岡大学教員データベース（新谷政己）
  • URL: https://tdb.shizuoka.ac.jp/RDB/public/Default2.aspx?id=11068&l=0
• [Remarks] ResearchGate個人ページ
  • URL: https://www.researchgate.net/profile/Masaki_Shintani
• [Remarks] Researchmap個人ページ
  • URL: http://researchmap.jp/shintani-masaki/?lang=english
• [Remarks] 金原・新谷研究室ホームページ
  • URL: http://kimbara-shintani.eng.shizuoka.ac.jp/~tmshint/
• [Remarks] 金原・新谷研究室
  • URL: http://kimbara-shintani.eng.shizuoka.ac.jp/~tmshint/
• [Remarks] researchmap
  • URL: http://researchmap.jp/shintani-masaki/?lang=japanese
• [Remarks] researchgate
  • URL: https://www.researchgate.net/profile/Masaki_Shintani