Understanding a mechanism regulating cellular differentiation using rice embryo.(Fostering Joint International Research)

• Project/Area Number: 15KK0279
• Research Category: Fund for the Promotion of Joint International Research (Fostering Joint International Research)
• Allocation Type: Multi-year Fund
• Research Field: Science in genetics and breeding
• Research Institution: National Institute of Genetics
• Principal Investigator: Yutaka Sato 国立遺伝学研究所, ゲノム・進化研究系, 教授 (40345872)
• Project Period (FY): 2016 – 2019
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
• Keywords: イネ / トウモロコシ / 胚

Outline of Final Research Achievements

In order to understand the conservation and diversities of molecular mechanism governing plant embryogenesis, I tried to compare embryogenesis of rice and maize through identification of similar set of mutations between them. I screened gle-like mutants in maize and identified several causal genes that give rise to gle phenotype. It turned out that all the causal mutations for gle found in maize are not the one previously identified in rice gle mutations. This suggests that embryo mutations recovered in maize and rice look similar but the pathway identified through these mutations are different. This is possibly due to the difference in genetic redundancy in these pathways. Thus, it is worth analyzing the embryo mutant in different plant materials so that whole pivotal pathways for plant embryogenesis become elucidated.

Academic Significance and Societal Importance of the Research Achievements

本研究により、日本のイネ研究者が主導する植物発生学と、米国のトウモロコシ用いた植物発生学研究者との国際的な連携を通して、同じイネ科作物であるイネとトウモロコシの発生の共通性と特異性を議論することができるようになった。今後、シロイヌナズナ一辺倒の現在の偏った植物発生学をより幅広い考察が可能な学問分野へと発展させることが期待される。

Research Products

• [Int'l Joint Research] フロリダ大学(米国) (2017)
• [Journal Article] Evolution and diversity of the wild rice Oryza officinalis complex, across continents genome types, and ploidy levels (2020)
  • Author(s): Matt Shenton, Masaaki Kobayashi, Shin Terashima, Hajime Ohyanagi, Dario Copetti, Tania Hernandez-Hernandez, Jianwei Zhang, Nobuko Ohmido, Masahiro Fujita, Atsushi Toyoda, Hiroshi Ikawa, Asao Fujiyama, Hiroyasu Furuumi, Toshie Miyabayashi, Takahiko Kubo, David Kudrna, Rod Wing, Kentaro Yano, Ken-ichi Nonomura et al.
  • Journal Title: Genome Biology and Evolution Volume: 12 Pages: 413-428
  • DOI: 10.1093/gbe/evaa037
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] RNAi of the sesquiterpene cyclase gene for phytoalexin production impairs pre‐ and post‐invasive resistance to potato blight pathogens (2019)
  • Author(s): Yoshioka, M., Adachi, I., Sato, Y., Doke, N., Kondo, T. and Yoshioka, H.
  • Journal Title: Molecular Plant Pathology Volume: 印刷中 Issue: 7 Pages: 907-922
  • DOI: 10.1111/mpp.12802
  • Peer Reviewed / Open Access
• [Journal Article] Affinity‐based high‐resolution analysis of DNA binding by VASCULAR‐RELATED NAC‐DOMAIN7 via fluorescence correlation spectroscopy (2019)
  • Author(s): Tamura Taizo、Endo Hitoshi、Suzuki Atsunobu、Sato Yutaka、Kato Ko、Ohtani Misato、Yamaguchi Masatoshi、Demura Taku
  • Journal Title: The Plant Journal Volume: 100 Issue: 2 Pages: 298-313
  • DOI: 10.1111/tpj.14443
  • Peer Reviewed / Open Access
• [Journal Article] Specification of basal region identity after asymmetric zygotic division requires mitogen-activated protein kinase 6 in rice (2019)
  • Author(s): Ishimoto K., Sohonahra S., Kishi-Kaboshi M., Itoh J-i., Hibara K-i., Sato Y., Watanabe T., Abe K., Miyao A., Nosaka-Takahashi M., Suzuki T., Ta N. K., Shimizu-Sato S., Suzuki T., Toyoda A., Takahashi H., Nakazono M., Nagato Y., Hirochika H., Sato Y.
  • Journal Title: Development Volume: 146
  • DOI: 10.1242/dev.176305
  • Peer Reviewed / Open Access
• [Journal Article] Genetic analysis of rice mutants responsible for narrow leaf phenotype and reduced vein number (2016)
  • Author(s): Kubo, F.C., Yasui, Y., Kumamaru, T., Sato, Y., and Hirano, H.-Y.
  • Journal Title: Genes & Genetic Systems Volume: 91 Issue: 4 Pages: 235-240
  • DOI: 10.1266/ggs.16-00018
  • NAID: 130005450049
  • ISSN: 1341-7568, 1880-5779
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] イネ胚形成におけるETT転写因子の下流で機能する転写因子群の解析 (2019)
  • Author(s): 高橋(野坂)実鈴、鈴木 俊哉、佐藤(志水)佐江、Nhung Ta、髙橋 宏和、鈴木 孝征、豊田 敦、中園 幹生、佐藤 豊
  • Organizer: 第60回日本植物生理学会年会
• [Presentation] イネ胚乳の発生におけるプラスチドシグナリングの関与 (2019)
  • Author(s): 加藤大和、深井麻央、阿南秀、佐藤豊、志水佐江、北野英己、小林裕子、小林一成、武田真、服部束穂
  • Organizer: 第60回日本植物生理学会年会
• [Presentation] Damage-associated Pep peptides influence root architecture and microbiome in rice (2019)
  • Author(s): Masako Fuji, Yuniar Devi Utami, Shigetaka Yasuda, Rena Tani, Yuichi Hongoh, Yutaka Sato, Yusuke Saijo
  • Organizer: 第60回日本植物生理学会年会
• [Presentation] Field Analysis for structural dynamics of rice associated microbiome (2019)
  • Author(s): Yuniar Devi Utami, Masako Fuji, Yukiko Shimizu, Yuichi Hongoh, Yutaka Sato, Yusuke Saijo
  • Organizer: 第60回日本植物生理学会年会
• [Presentation] Plant embryogenesis: Conservations and divergencies envisioned form rice research (2018)
  • Author(s): Yutaka Sato
  • Organizer: 16th International Symposium on Rice Functional Genomics
  • Int'l Joint Research / Invited
• [Presentation] NBRPイネリソースの紹介 (2018)
  • Author(s): 佐藤豊
  • Organizer: 日本育種学会第134回講演会
• [Book] Rice Genomics, Genetics and Breeding (2018)
  • Author(s): Yutaka Sato, Misuzu Nosaka-Takahashi, Toshiya Suzuki, Sae Shimizu-Sato
  • Total Pages: 15
  • Publisher: Springer
  • ISBN: 9789811074608
• [Book] ノンコーディングRNA (2016)
  • Author(s): 都筑正行、濱田隆宏、佐藤 豊
  • Total Pages: 372
  • Publisher: 化学同人