| Outline of Final Research Achievements |
Ibaraki virus (IBAV) is a member of the epizootic hemorrhagic disease virus serogroup, which belongs to the Orbivirus genus of the Reoviridae family. Previously, we developed reverse genetics system (RG) for IBAV, to clarify the molecular mechanism of orbivirus replication. We also found several cell lines, in which, the replication of IBAV was suppressed. Here, using newly developed plasmid-based RG for two other orbiviruses, bluetongue virus (BTV) and Muko virus (MUV), fluorescent-labeled (FL-) BTV and MUV were generated. Co-infection assay using FL-BTV and FL-MUV together with FL-IBAV revealed the fuzzy self-recognition mechanisms in virus inclusion body. In addition, we demonstrated that some mechanisms of a unique protein, VP6, were shared among orbiviruses. Further, the replication of midge-borne orbiviruses, not tick-bone virus, was likely to be suppressed in several cell lines. We also tried to reveal VP6 structure using Cryo-EM technique, which was unfortunately failed.
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