• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Preparation of functional glyco-polymers and analysis of their cellular immune activities to develop anti-pollinosis drugs(Fostering Joint International Research)

Research Project

Project/Area Number 15KK0282
Research Category

Fund for the Promotion of Joint International Research (Fostering Joint International Research)

Allocation TypeMulti-year Fund
Research Field Applied molecular and cellular biology
Research InstitutionOkayama University

Principal Investigator

MAEDA Megumi  岡山大学, 環境生命科学研究科, 准教授 (20434988)

Research Collaborator VAN DAMME Els J.M.  Faculty of Bioscience Engineering, Ghent University, Department of Molecular Biotechnology, Prof.
Rahman Ziaur  Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Bangladesh Atomic Energy Commission
Project Period (FY) 2015 – 2017
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Keywords遊離N-グリカン / 糖鎖結合タンパク質 / レクチン / 糖鎖ポリマー / PNGase / ENGase / 小胞体品質管理 / ルイスa抗原 / FNGs / ハイマンノース型糖鎖 / 複合型糖鎖 / ポリ-γ-L-グルタミン酸 / 核細胞質レクチン / 環境ストレス / aPNGase / pseudomonas syringae / 遊離型N-グリカン / 糖鎖 / 蛋白質 / 生体分子 / シグナル伝達 / 免疫学
Outline of Final Research Achievements

Naturally occurring free N-glycans (FNGs) derived from glycoproteins/glycopeptides by the action of peptide: N-glycanase (PNGase) and endo-β-N-acetylglucosaminidase (ENGase) have been postulated to act as signaling molecules stimulating plant growth or fruit ripening. To elucidate the physiological role of FNGs in plants, we have analyzed the sensitivity of the A.thaliana mutants, ENGase-DKO and acidic PNGase-DKO, to abiotic (NaCl treatment) and biotic (P. syringae infection) stresses. Since it is believed that nucleocytoplasmic lectins function in the plant stress response, synthesized glycopolymers were used to study the interaction between FNGs and lectins in plant cells.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (34 results)

All 2018 2017 2016 Other

All Int'l Joint Research (1 results) Journal Article (7 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 6 results,  Open Access: 7 results,  Acknowledgement Compliant: 1 results) Presentation (23 results) (of which Int'l Joint Research: 7 results,  Invited: 1 results) Remarks (3 results)

  • [Int'l Joint Research] Faculty of Bioscience Engineering, Ghent University(ベルギー)2016

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Novel assay system for acidic Peptide:N-glycanase (aPNGase) activity in crude plant extract.2018

    • Author(s)
      Uemura R, Ogura M, Matsumaru C, Akiyama T, Maeda M, Kimura Y.
    • Journal Title

      Biosci Biotechnol Biochem.

      Volume: 印刷中 Issue: 7 Pages: 1172-1175

    • DOI

      10.1080/09168451.2018.1459464

    • NAID

      120006619340

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Molecular characterization of second tomato α1,3/4-fucosidase (α-Fuc'ase Sl-2), a member of glycosyl hydrolase family 29 active toward the core α1,3-fucosyl residue in plant N-glycans.2018

    • Author(s)
      Rahman Z, Tsujimori Y, Maeda M, Hossain A, Ishimizu T, Kimura Y.
    • Journal Title

      J Biochem.

      Volume: 印刷中 Issue: 1 Pages: 53-63

    • DOI

      10.1093/jb/mvy029

    • NAID

      40021625325

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Degradation pathway of plant complex-type N-glycans: identification and characterization of a key α1,3-fucosidase from glycoside hydrolase family 29.2018

    • Author(s)
      Kato S, Hayashi M, Kitagawa M, Kajiura H, Maeda M, Kimura Y, Igarashi K, Kasahara M, Ishimizu T.
    • Journal Title

      Biochem J.

      Volume: 475 Issue: 1 Pages: 305-317

    • DOI

      10.1042/bcj20170106

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Occurrence of complex type free N-glycans with a single GlcNAc residue at the reducing termini in the fresh-water plant, Egeria densa2017

    • Author(s)
      Maeda Megumi、Ebara Natsuki、Tani Misato、Vavricka Christopher J.、Kimura Yoshinobu
    • Journal Title

      Glycoconj. J.

      Volume: 34 Issue: 2 Pages: 1405-1408

    • DOI

      10.1007/s10719-016-9758-z

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Ginkgo biloba α-fucosidase with activity towards plant complex type N-glycans containing the Lewis a epitope: Purification and characterization.2017

    • Author(s)
      Itano S., Maeda M., Rahman M.Z., and Kimura Y.
    • Journal Title

      Scientific Reports of the Faculty of Agriculture, Okayama University

      Volume: 106 Pages: 5-12

    • NAID

      120005970803

    • Related Report
      2016 Research-status Report
    • Open Access / Int'l Joint Research
  • [Journal Article] Molecular characterization of tomato α1,3/4-fucosidase, a member of glycosyl hydrolase family 29, involved in degradation of plant complex type N-glycans.2017

    • Author(s)
      Rahman, M.D., Maeda, M., Itano, S., Hossain, M.A., Ishimizu, T., Kimura, Y.
    • Journal Title

      J. Biochem.

      Volume: 161 Pages: 421-432

    • DOI

      10.1093/jb/mvw089

    • NAID

      40021219805

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Structural features of N-glycans linked to glycoproteins expressed in three kinds of water plants: Predominant occurrence of the plant complex type N-glycans bearing Lewis a epitope.2016

    • Author(s)
      Maeda M., Tani M., Yoshiie T., Vavricka CJ., Kimura Y.
    • Journal Title

      Carbohydr Res.

      Volume: 435 Pages: 50-57

    • DOI

      10.1016/j.carres.2016.09.008

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] 酸性PNGase過剰発現トマト(T4世代)のPNGase 活性と遊離糖鎖構造解析2018

    • Author(s)
      小椋美夏子,前田恵,木村吉伸
    • Organizer
      日本農芸化学会中四国支部第50回記念講演会(例会)広島大学東広島キャンパス
    • Related Report
      2017 Annual Research Report
  • [Presentation] 新規人工糖鎖ポリマーを利用したダイズ核/細胞質レクチンの同定2018

    • Author(s)
      高瀬美穂,前田恵,Els JM Van Damme, 木村吉伸
    • Organizer
      日本農芸化学会中四国支部第50回記念講演会(例会)広島大学東広島キャンパス
    • Related Report
      2017 Annual Research Report
  • [Presentation] 小胞体関連分解に関わる糖鎖認識タンパク質を欠損した A. thaliana が産生する遊離N-グリカン(FNGs)の構造解析2018

    • Author(s)
      古田佳織,前田恵,木村吉伸
    • Organizer
      日本農芸化学会中四国支部第50回記念講演会(例会)広島大学東広島キャンパス
    • Related Report
      2017 Annual Research Report
  • [Presentation] 脱グリコシル化酵素を欠損したA. thalianaの構築と遊離N-グリカン構造解析2018

    • Author(s)
      上村 亮太,前田 恵,三﨑 亮,藤山 和仁,木村 吉伸
    • Organizer
      日本農芸化学会中四国支部第50回記念講演会(例会)広島大学東広島キャンパス
    • Related Report
      2017 Annual Research Report
  • [Presentation] トマト導管液中に存在するLea抗原含有N-グリカン代謝関連酵素の活性と遊離N-グリカン構造解析2018

    • Author(s)
      辻森祐太,前田恵,木村吉伸
    • Organizer
      日本農芸化学会中四国支部第50回記念講演会(例会)広島大学東広島キャンパス
    • Related Report
      2017 Annual Research Report
  • [Presentation] 酸性PNGase 遺伝子を完全にノックアウトしたA.thaliana 中のPNGase 活性と遊離 N-グリカンの構造特性2017

    • Author(s)
      上村亮太,前田 恵,秋山 剛, 三﨑 亮,藤山和仁,木村吉伸
    • Organizer
      日本農芸化学会2017年度大会
    • Place of Presentation
      京都女子大学
    • Year and Date
      2017-03-18
    • Related Report
      2016 Research-status Report
  • [Presentation] 酸性PNGase過剰発現トマトの果実成熟に関わる遺伝子の発現解析2017

    • Author(s)
      藤川真奈,前田 恵,松丸千紘,中野龍平,梶浦裕之,三崎 亮,藤山和仁,木村吉伸
    • Organizer
      支部創立15周年記念 日本農芸化学会中四国第47回講演会(支部例会)
    • Place of Presentation
      島根大学 松江キャンパス
    • Year and Date
      2017-01-28
    • Related Report
      2016 Research-status Report
  • [Presentation] 酸性ペプチド: N-グリカナーゼ遺伝子ノックアウト植物に存在する遊離N-グリカン構造解析2017

    • Author(s)
      上村亮太,前田 恵,秋山 剛,三崎 亮, 藤山和仁,木村吉伸
    • Organizer
      支部創立15周年記念 日本農芸化学会中四国第47回講演会(支部例会)
    • Place of Presentation
      島根大学 松江キャンパス
    • Year and Date
      2017-01-28
    • Related Report
      2016 Research-status Report
  • [Presentation] 昆虫細胞を用いたトマトα1,3/4-フコシダーゼII (Fuc’ase Sl2) の発現系構築2017

    • Author(s)
      前田 恵,辻森祐太,Rahman Md. Ziaur,木村吉伸
    • Organizer
      支部創立15周年記念 日本農芸化学会中四国第47回講演会(支部例会)
    • Place of Presentation
      島根大学 松江キャンパス
    • Year and Date
      2017-01-28
    • Related Report
      2016 Research-status Report
  • [Presentation] 小胞体品質管理に関わるα-マンノシダーゼ二重欠損体の遊離糖鎖構造解析2017

    • Author(s)
      前田 恵,古田佳織,木村吉伸
    • Organizer
      支部創立15周年記念 日本農芸化学会中四国第47回講演会(支部例会)
    • Place of Presentation
      島根大学 松江キャンパス
    • Year and Date
      2017-01-28
    • Related Report
      2016 Research-status Report
  • [Presentation] 酸性PNGase活性を欠損させたA. thalianaの表現型と内在する遊離N-グリカンの構造特性2017

    • Author(s)
      上村 亮太、前田 恵、三崎 亮、藤山 和仁、木村 吉伸
    • Organizer
      2017年度生命科学系学会合同年次大会(ConBio2017)神戸ポートアイランド
    • Related Report
      2017 Annual Research Report
  • [Presentation] Plant free N-glycans as signaling molecules: Putative functions and Receptor proteins.2017

    • Author(s)
      Megumi Maeda
    • Organizer
      「国際シンポジウム-Biomolecules controlling cellular function and environmental adaptation in plants-」(岡山大学創立五十周年記念館 2F大会議室)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Application of Glycopolymers Bearing Multivalent N-Glycans for Detection of Lectins2017

    • Author(s)
      Megumi Maeda, Yoshinobu Kimura, Els J.M. Van Damme
    • Organizer
      28th Joint Glycobiology Meeting, RWTH, Aachen, Germany
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] MOLECULAR CHARACTERIZATION SOLANUM LYCOPERSICUM Α1,3/4-FUCOSIDASE II (Α-FUC’ASE SL-II) INVOLVED IN DEGRADATION OF PLANT COMPLEX TYPE N-GLYCAN2017

    • Author(s)
      Yuta Tsujimori , M. Rahman, M. Maeda, M. Hossain, Y. Kimura.
    • Organizer
      19th European Carbohydrate Symposium (EUROCARB), Barcelona CCIB, Spain
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ENZYME ASSAY AND FREE N-GLYCANS STRUCTURAL ANALYSIS OF ACIDIC PEPTIDE: N-GLYCANASE (APNGASE) KNOCKOUT PLANT2017

    • Author(s)
      Ryota Uemura, M. Maeda, T. Akiyama, R. Misaki, K. Fujiyama, Y. Kimura.
    • Organizer
      19th European Carbohydrate Symposium (EUROCARB), Barcelona CCIB, Spain
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Accumulation of GN1-type plant complex type free N-glycans in α1,3/4-fucosidase knockout mutant of A. thaliana2017

    • Author(s)
      M. Maeda, S. Takata, T. Ishimizu, E.J.M. Van Damme, Y. Kimura
    • Organizer
      12th Carbohydrate Bioengineering Meeting(The CBM12)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Significant decrease in the amount of GN1-type free N-glycans in ER-α-mannosidase knocked-out A. thaliana2017

    • Author(s)
      K. Furuta, M. Maeda, R. Strasser, Y. Kimura
    • Organizer
      12th Carbohydrate Bioengineering Meeting(The CBM12)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] トマト酸性Peptide: N-glycanase過剰発現体の遺伝子発現解析」(Gene expression analysis of tomato plant overexpressing acidic peptide: N-glycanase2016

    • Author(s)
      藤川真奈,前田恵,松丸千紘,中野龍平,梶浦裕之,三崎亮,藤山和仁,木村吉伸
    • Organizer
      第89回日本生化学会大会
    • Place of Presentation
      仙台国際センター
    • Year and Date
      2016-09-26
    • Related Report
      2016 Research-status Report
  • [Presentation] 酸性ペプチド:N-グリカナーゼ(aPNGase)候補遺伝子ノックアウト植物体のaPNGase 活性,遊離糖鎖構造解析及びストレス感受性解析2016

    • Author(s)
      上村亮太,秋山 剛,前田 恵,三崎 亮,藤山和仁,木村吉伸
    • Organizer
      日本農芸化学会2016年度中四国支部大会(第46回講演会)
    • Place of Presentation
      高知県立県民文化ホール グリーンホール,高知大学 朝倉キャンパス
    • Year and Date
      2016-09-26
    • Related Report
      2016 Research-status Report
  • [Presentation] Molecular characterization of second tomato α1, 3/4-fucosidase (α-Fuc’ase S1-2) involved in degradation of plant complex type N-glycans.2016

    • Author(s)
      Md. Ziaur Rahman,Megumi Maeda,Satsuki Itano,Yoshinobu Kimura
    • Organizer
      日本農芸化学会2016年度中四国支部大会(第46回講演会)
    • Place of Presentation
      高知県立県民文化ホール グリーンホール,高知大学 朝倉キャンパス
    • Year and Date
      2016-09-26
    • Related Report
      2016 Research-status Report
  • [Presentation] Effect of Pseudomonas syringae Infection on ENGase or Acidic PNGase Knockout Arabidopsis thaliana Plants.2016

    • Author(s)
      Megumi Maeda, Ryota Uemura, Tsuyoshi Akiyama, Els J.M. Van Damme and Yoshinobu Kimura
    • Organizer
      10th Tri-National Arabidopsis Meeting
    • Place of Presentation
      Vienna, Austria
    • Year and Date
      2016-09-14
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 植物コンプレックス型糖鎖の分解経路の解明:1,3-フコシダーゼの同定と解析2016

    • Author(s)
      加藤俊,林めぐみ,北川真衣,前田恵,木村吉伸,五十嵐圭日子,笠原賢洋,石水毅
    • Organizer
      第35回日本糖質学会年会
    • Place of Presentation
      高知市文化プラザ かるぽーと
    • Year and Date
      2016-09-03
    • Related Report
      2016 Research-status Report
  • [Presentation] 酸性ペプチド:N-グリカナーゼ(aPNGase)過剰発現トマトの構築と表現型解析2016

    • Author(s)
      松丸千紘,前田恵,村田翔平,中野龍平,中村浩介,三崎亮,梶浦裕之,藤山和仁,木村吉伸
    • Organizer
      第35回日本糖質学会年会
    • Place of Presentation
      高知市文化プラザ かるぽーと
    • Year and Date
      2016-09-03
    • Related Report
      2016 Research-status Report
  • [Remarks] 糖鎖機能化学研究室

    • URL

      http://www.okayama-u.ac.jp/user/agr/profile/nougaku01_3.html

    • Related Report
      2017 Annual Research Report
  • [Remarks] 岡山大学 研究者総覧

    • URL

      http://soran.cc.okayama-u.ac.jp/view?l=ja&u=ec49c68c89dc75d674506e4da22f6611#item_shuyogsk_highlight_2

    • Related Report
      2017 Annual Research Report
  • [Remarks] 岡山大学農学部糖鎖機能化学研究室

    • URL

      http://www.okayama-u.ac.jp/user/agr/profile/nougaku01_3.html

    • Related Report
      2015 Research-status Report

URL: 

Published: 2016-10-04   Modified: 2019-03-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi