Analysis of expression mechanisms and adherence functions of streptococcal pili(Fostering Joint International Research)
| Project/Area Number |
15KK0306
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| Research Category |
Fund for the Promotion of Joint International Research (Fostering Joint International Research)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2016 – 2019
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000)
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| Keywords | レンサ球菌 / 線毛 / 化膿レンサ球菌 |
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| Outline of Final Research Achievements |
Streptococcus pyogenes produces a variety of pili and thermosensitive expression of pilus genes was previously observed in a specific serotype strain. To reveal the mechanism involved, pilus expression analysis of clinical isolates was conducted. Thermosensitive pilus production was only observed in strains possessing the nra gene encoding a transcriptional regulator. Additionally, analysis using mutant strains indicated that the Nra protein is a positive regulator of pilus genes and showed a relationship of its intracellular level with pilus production. The translational efficiency of nra mRNA was increased when the temperature was decreased and a putative stem loop structure within the coding region of nra mRNA was also shown to be involved in that. Thus, during the initial infection step, S. pyogenes may adapt to the external body surface temperature, and modulate both the translational efficiency of nra and expression levels of pili required for initial adherence to host tissues.
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| Academic Significance and Societal Importance of the Research Achievements |
化膿レンサ球菌の感染初期に機能する細菌因子の産生量を規定する仕組みとして，転写を促進する因子のmRNAが環境温度の変化により構造を変化させ，その翻訳が調節されることが推察された．環境温度を感知するmRNAの部位は，主に報告されてきた非翻訳領域ではなく，翻訳領域にあるという，本菌では初めて明らかにされたmRNAによる温度感知機構であった．さらに，臨床現場で用いられてきた化膿レンサ球菌のTタイピング法において低温培養が必要とされてきたが，その理由の一端が明らかになった．
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Report
(5 results)
Research Products
(68 results)
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[Journal Article] Allergic conversion of protective mucosal immunity against nasal bacteria in patients with chronic rhinosinusitis with nasal polyposis.2019
Author(s)
Takeda K, Sakakibara S, Yamashita K, Motooka D, Nakamura S, El Hussien MA, Katayama J, Maeda Y, Nakata M, Hamada S, Standley DM, Hayama M, Shikina T, Inohara H, Kikutani H.
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Journal Title
J Allergy Clin Immunol
Volume: 143
Issue: 3
Pages: 1163-1175
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Streptococcus sanguinis non-coding csRNAs negatively regulate expression of type IV pilus retraction ATPase PilT and biofilm formation2018
Author(s)
Ota C, Morisaki H, Nakata M, Arimoto T, Fukamachi H, Kataoka H, Masuda Y, Suzuki N, Miyazaki T, Okahashi N, Kuwata H
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Journal Title
Infect Immun
Volume: 86
Issue: 3
Pages: 1-13
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Streptococcus sanguinis induces neutrophil cell death by production of hydrogen peroxide.2017
Author(s)
Sumioka, R., Nakata, M., Okahashi, N., Li, Y., Wada, S., Yamaguchi, M., Sumitomo, T., Hayashi, M., Kawabata, S.
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Journal Title
PLoS One
Volume: 12
Issue: 2
Pages: e0172223-e0172223
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Evolutionary inactivation of a sialidase ingroup B Streptococcus.2016
Author(s)
Yamaguchi, M., Hirose, Y., Nakata, M., Uchiyama, S., Yamaguchi, Y., Goto, K., Sumitomo, T., Lewis, A., Kawabata, S., Nizet, V.
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Journal Title
Scientific Reports
Volume: 6
Issue: 1
Pages: 28852-28852
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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