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Characterization of exosomes released from Epstein-Barr virus-infected cells(Fostering Joint International Research)

Research Project

Project/Area Number 15KK0324
Research Category

Fund for the Promotion of Joint International Research (Fostering Joint International Research)

Allocation TypeMulti-year Fund
Research Field Virology
Research InstitutionHokkaido University

Principal Investigator

Nanbo Asuka  北海道大学, 医学研究院, 准教授 (60359487)

Research Collaborator Sugden Bill  ウィスコンシン大学マディソン校, マッカードル癌研究所, 教授
OHBA Yusuke  
YOSHIYAMA Hironori  
KATANO Harutaka  
NODA Takeshi  
OHASHI Makoto  
Project Period (FY) 2016 – 2018
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000)
KeywordsEpstein-Barrウイルス / エキソソーム / microRNA / EBV関連がん / miRNA / 次世代シーケンス / Epstein-Barr virus / マイクロRNA
Outline of Final Research Achievements

Infection of Epstein-Barr virus (EBV), a ubiquitous human herpesvirus, is associated with various malignancies. EBV-infected cells have been shown to secrete extracellular micro vesicles, exosomes, which possess various functions by transferring sets of proteins, lipids and RNAs to the recipient cells.
Although accumulating evidence demonstrates that exosomes released form EBV-infected cells transfer viral factors including microRNAs to recipient cells, little is known about their roles in vital lifecycle. Here, we found that the biogenesis of exosomes is upregulated in infected cells. We also observed that several specific microRNAs were predominantly incorporated in the exosomes released from infected cells, potentially contributing to phenotypic changes in cells receiving these exosomes.

Academic Significance and Societal Importance of the Research Achievements

近年、がん細胞から放出されたエキソソームが、特定のマイクロRNA を標的細胞に供給することで、がんの増殖や転移に関与することが報告されつつある。また,特定のマイクロRNA ががんの早期段階で発現し、エキソソームを介して血液中に放出される現象を利用し、血液中のマイクロRNA の量を測定することで、がんの早期発見や診断法に応用できるものと期待されている。今後、本研究を発展させることで、EBV関連がん発症メカニズムの詳細な解明につながることが期待される。また、本研究で同定された、エキソソームに濃縮される特定のマイクロRNA を対象として、EBV関連がんの診断法の実現につながることが期待される。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (30 results)

All 2019 2018 2017 2016 Other

All Int'l Joint Research (1 results) Journal Article (13 results) (of which Int'l Joint Research: 6 results,  Peer Reviewed: 12 results,  Open Access: 10 results,  Acknowledgement Compliant: 1 results) Presentation (13 results) (of which Int'l Joint Research: 4 results,  Invited: 2 results) Book (2 results) Remarks (1 results)

  • [Int'l Joint Research] ウィスコンシン大学マディソン校マッカードル癌研究所(米国)2016

    • Year and Date
      2016-06-01
    • Related Report
      2018 Annual Research Report
  • [Journal Article] A Peptide Derived from Phosphoinositide 3-kinase Inhibits Endocytosis and Influenza Virus Infection2019

    • Author(s)
      Fujioka Y, Satoh AO, Horiuchi K, Fujioka M, Tsutsumi K, Sasaki J, Nepal P, Kashiwagi S, Paudel S, Nishide S, Nanbo A, Sasaki T, Ohba Y
    • Journal Title

      Cell Structure and Function

      Volume: 44 Issue: 1 Pages: 61-74

    • DOI

      10.1247/csf.19001

    • NAID

      130007636814

    • ISSN
      0386-7196, 1347-3700
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Extracellular Vesicles in Epstein-Barr Virus' Life Cycle and Pathogenesis2019

    • Author(s)
      Zhao M, Nanbo A, Sun L, Lin Z
    • Journal Title

      Microorganisms

      Volume: 7 Issue: 2 Pages: 48-48

    • DOI

      10.3390/microorganisms7020048

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A Sialylated Voltage-Dependent Ca2+ Channel Binds Hemagglutinin and Mediates Influenza A Virus Entry into Mammalian Cells2018

    • Author(s)
      Fujioka Y, Nishide S, Ose T, Suzuki T, Kato I, Fukuhara H, Fujioka M, Horiuchi K, Satoh AO, Nepal P, Kashiwagi S, Wang J, Horiguchi M, Sato Y, Paudel S, Nanbo A, Miyazaki T, Hasegawa H, Maenaka K, Ohba Y
    • Journal Title

      Cell Host Microbe

      Volume: 23 Issue: 6 Pages: 809-818

    • DOI

      10.1016/j.chom.2018.04.015

    • NAID

      120006643588

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] The Role of Transforming Growth Factor β in Cell-to-Cell Contact-Mediated Epstein-Barr Virus Transmission2018

    • Author(s)
      Nanbo A, Ohashi M, Yoshiyama H, Ohba Y
    • Journal Title

      Front Microbiol

      Volume: 9 Pages: 984-984

    • DOI

      10.3389/fmicb.2018.00984

    • NAID

      120006489803

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Infection of Epstein?Barr Virus in Type III Latency Modulates Biogenesis of Exosomes and the Expression Profile of Exosomal miRNAs in the Burkitt Lymphoma Mutu Cell Lines2018

    • Author(s)
      Nanbo Asuka、Katano Harutaka、Kataoka Michiyo、Hoshina Shiho、Sekizuka Tsuyoshi、Kuroda Makoto、Ohba Yusuke
    • Journal Title

      Cancers

      Volume: 10 Issue: 7 Pages: 237-237

    • DOI

      10.3390/cancers10070237

    • NAID

      120006491456

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Budding of Ebola Virus Particles Requires the Rab11-Dependent Endocytic Recycling Pathway2018

    • Author(s)
      Nanbo A, Ohba Y
    • Journal Title

      J Infect Dis

      Volume: 218 Issue: suppl_5 Pages: S388-S396

    • DOI

      10.1093/infdis/jiy460

    • NAID

      120006773327

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Molecular Mechanism of Externalization of Phosphatidylserine on the Surface of Ebola Virus Particles2018

    • Author(s)
      Nanbo A, Kawaoka Y
    • Journal Title

      DNA Cell Biol

      Volume: 38 Issue: 2 Pages: 115-120

    • DOI

      10.1089/dna.2018.4485

    • NAID

      130007812446

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ebola virus requires a host scramblase for externalization of phosphatidylserine on the surface of viral particles2018

    • Author(s)
      Nanbo Asuka、Maruyama Junki、Imai Masaki、Ujie Michiko、Fujioka Yoichiro、Nishide Shinya、Takada Ayato、Ohba Yusuke、Kawaoka Yoshihiro
    • Journal Title

      PLoS Pathog

      Volume: 14 Issue: 1 Pages: e1006848-e1006848

    • DOI

      10.1371/journal.ppat.1006848

    • NAID

      120006416019

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Epstein-Barr Virus Acquires Its Final Envelope on Intracellular Compartments With Golgi Markers2018

    • Author(s)
      Nanbo Asuka、Noda Takeshi、Ohba Yusuke
    • Journal Title

      Front Microbiol

      Volume: 9 Pages: 454-454

    • DOI

      10.3389/fmicb.2018.00454

    • NAID

      120006416029

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Inside the Cell: Assembly of Filoviruses2017

    • Author(s)
      Kolesnikova Larissa、Nanbo Asuka、Becker Stephan、Kawaoka Yoshihiro
    • Journal Title

      Curr Top Microbiol Immunol

      Volume: 411 Pages: 353-380

    • DOI

      10.1007/82_2017_15

    • ISBN
      9783319689463, 9783319689487
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Journal Article] Improved FRET Biosensor for the Measurement of BCR-ABL Activity in Chronic Myeloid Leukemia Cells2017

    • Author(s)
      M. Horiguchi, M. Fujioka, T. Kondo, Y. Fujioka, X. Li, K. Horiuchi, A.O. Satoh, P. Nepal, S. Nishide, A. Nanbo, T. Teshima and Y. Ohba.
    • Journal Title

      Cell Structure and Function

      Volume: 42 Issue: 1 Pages: 15-26

    • DOI

      10.1247/csf.16019

    • NAID

      130005308488

    • ISSN
      0386-7196, 1347-3700
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Epstein-Barr virus exploits host endocytic machinery for cell-to-cell viral transmission rather than a virological synapse.2016

    • Author(s)
      A. Nanbo, K. Kachi, H. Yoshiyama and Y. Ohba.
    • Journal Title

      J. Gen. Virol.

      Volume: 97 Issue: 11 Pages: 2989-3006

    • DOI

      10.1099/jgv.0.000605

    • NAID

      120006360051

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Fcγ-receptor IIa-mediated Src Signaling Pathway Is Essential for the Antibody-Dependent Enhancement of Ebola Virus Infection.2016

    • Author(s)
      Furuyama W, Marzi A, Carmody AB, Maruyama J, Kuroda M, Miyamoto H, Nanbo A, Manzoor R, Yoshida R, Igarashi M, Feldmann H, Takada A.
    • Journal Title

      PLoS Pathog.

      Volume: 12(12)

    • NAID

      120005981447

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] Epstein-BarrウイルスIII型潜伏感染はエクソソーム産生ならびに内包されるmicroRNAの発現様式を変動する2018

    • Author(s)
      南保明日香,片野晴隆,片岡紀代,保科しほ,関塚剛史,黒田誠,Bill Sugden,大場雄介
    • Organizer
      第32回ヘルペスウイルス研究会(ホテル福岡ガーデンパレス、福岡市)
    • Related Report
      2018 Annual Research Report
  • [Presentation] 細胞間接触を介したEpstein-Barrウイルス感染機構におけるTGF-bの役割2018

    • Author(s)
      南保明日香,大橋誠,吉山裕規,大場雄介
    • Organizer
      第15回EBV研究会(山口大学医学部、宇部市)
    • Related Report
      2018 Annual Research Report
  • [Presentation] エボラウイルスの細胞内侵入機構の解明2018

    • Author(s)
      南保明日香
    • Organizer
      第30回北海道輸血シンポジウム(日本赤十字社北海道ブロック血液センター、札幌市)
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Understanding of molecular mechanism of Ebola virus entry2018

    • Author(s)
      南保明日香
    • Organizer
      第17回あわじしま感染症・免疫フォーラム(淡路夢舞台国際会議場、淡路島)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Budding of Ebola Virus Particles Requires the Rab11-Dependent Endocytic Recycling Pathway2018

    • Author(s)
      南保明日香,大場雄介
    • Organizer
      第66回日本ウイルス学会学術集会(京都テルサ、京都市)
    • Related Report
      2018 Annual Research Report
  • [Presentation] Epstein-Barr virus acquires its final envelope on intracellular compartments with Golgi markers2018

    • Author(s)
      Nanbo A, Noda T, Ohba Y
    • Organizer
      International Conference on EBV and KSHV (University of Wisconsin, Madison, Madison, USA)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] エボラウイルスエンベロープへのホスファチジルセリン集積に関与する宿主スクランブラーゼの同定2018

    • Author(s)
      南保明日香
    • Organizer
      7th Negative Strand Virus-Japan (サザンビーチホテル&リゾート沖縄, 糸満市)
    • Related Report
      2017 Research-status Report
  • [Presentation] Epstein-Barrウイルス粒子出芽機構に関する研究2017

    • Author(s)
      南保明日香,Bill Sugden,大場雄介
    • Organizer
      第31回ヘルペスウイルス研究会 (松江ニューアーバンホテル、松江市)
    • Related Report
      2017 Research-status Report
  • [Presentation] 細胞接触を介したEpstein-Barrウイルス感染機構における分子機構の解明2017

    • Author(s)
      南保明日香,加持邦弘,吉山裕規,大場雄介
    • Organizer
      第14回EBV研究会 (旭川グランドホテル、旭川市)
    • Related Report
      2017 Research-status Report
  • [Presentation] EBOV requires a host scramblase for its efficient entry2017

    • Author(s)
      Nanbo A,Maruyama J,Komatsu H,Watanabe T,Fujimuro M,Takada A,Ohba Y,Kawaoka Y
    • Organizer
      第65回日本ウイルス学会学術集会(大阪国際会議場、大阪市)
    • Related Report
      2017 Research-status Report
  • [Presentation] EBOV requires a host scramblase for its efficient entry2017

    • Author(s)
      Nanbo A, Maruyama J,Watanabe T,Fujimuro M,Ohba Y,Takada A,Kawaoka Y
    • Organizer
      9th International Symposium on Filoviruses (Welcome Hotel Marburg, Marburg)
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Epstein-Barr virus exploits host endocytic machinery for cell-to-cell viral transmission2016

    • Author(s)
      Nanbo A, Kachi K, Yoshiyama H, Ohba Y
    • Organizer
      17th International symposium on Epstein-Barr virus and associated disease
    • Place of Presentation
      University of Tower, Zurich
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Characterization of exosomes derived from Epstein-Barr virus-infected cells2016

    • Author(s)
      南保明日香、片野晴隆、飯笹久、吉山裕規、Bill Sugden、大場雄介
    • Organizer
      第64回日本ウイルス学会学術集会
    • Place of Presentation
      札幌コンベンションホール, 札幌
    • Related Report
      2016 Research-status Report
  • [Book] Marburg and Ebola Viruses: From Ecosystems to Molecules, Inside the Cell2017

    • Author(s)
      Kolesnikova L, Nanbo A, Kawaoka Y, and Becker S,
    • Publisher
      Springer
    • Related Report
      2016 Research-status Report
  • [Book] EBウイルス関連胃癌2016

    • Author(s)
      南保明日香
    • Total Pages
      5
    • Publisher
      診断と治療社
    • Related Report
      2016 Research-status Report
  • [Remarks] EBウイルス感染細胞がまき散らす粒子の性質を解明

    • URL

      https://www.hokudai.ac.jp/news/180806_pr.pdf

    • Related Report
      2018 Annual Research Report

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Published: 2016-10-04   Modified: 2020-03-30  

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