Investigation of Bone Marrow-derived Stem Cells in Periodontal Ligament(Fostering Joint International Research)

• Project/Area Number: 15KK0337
• Research Category: Fund for the Promotion of Joint International Research (Fostering Joint International Research)
• Allocation Type: Multi-year Fund
• Research Field: Prosthodontics/ Dental materials science and
• Research Institution: Niigata University
• Principal Investigator: Kaku Masaru 新潟大学, 医歯学系, 准教授 (30547542)
• Project Period (FY): 2016 – 2019
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000)
• Keywords: 歯根膜 / 組織幹細胞 / 血行性幹細胞 / 細胞系譜解析 / 神経堤細胞 / 蛍光プローブ / 細胞増殖 / 細胞トラッキング / 深部解析 / 蛍光イメージング

Outline of Final Research Achievements

Periodontal ligament plays important roles in the occlusal function therefore, its maintenance/regeneration is essential for achieving healthy longevity of life. It has been speculated that the growth factors and extracellular matrix proteins secreted by periodontal ligament-constituting cells regulate the maintenance/differentiation of periodontal ligament in a site-specific manner. However, the site-specificity of the periodontal ligament-constituting cells are still not well characterized. The present study revealed that not only the cells existing from the developmental stage but also the hematogenously supplied bone marrow-derived cells are present in the periodontal ligament. These cells contribute to the maintenance of periodontal ligament homeostasis.

Academic Significance and Societal Importance of the Research Achievements

本研究により，口腔機能に重要な歯根膜における，血行性幹細胞の供給と組織内の部位特異的な分化機構の一端が明らかとなった．本研究成果は歯根膜の維持機構の解明のみならず，歯根膜組織の再生法の開発という点からも重要である．歯根膜組織の再生には，組織内外に存在する幹細胞を，時と場に応じた細胞に分化させる技術が必要となる．本研究成果からも明らかなように，歯根膜組織中の細胞は，極めてヘテロな細胞集団であることから，より詳細な歯根膜構成細胞の特性解析が必要である．

Research Products

• [Int'l Joint Research] The University of Texas(米国) (2017)
  • Year and Date: 2017-05-28
• [Journal Article] Regeneration of Bone/ Periodontal ligament and Mechanical load (2019)
  • Author(s): 魚島 勝美, 加来 賢, 長澤 麻沙子
  • Journal Title: Annals of Japan Prosthodontic Society Volume: 11 Issue: 1 Pages: 14-19
  • DOI: 10.2186/ajps.11.14
  • NAID: 130007557989
  • ISSN: 1883-4426, 1883-6860
  • Peer Reviewed / Open Access
• [Journal Article] Response to Letter to the Editor: Concerns on modeling postmenopausal osteoporosis in young female rats (2019)
  • Author(s): Rocabado Juan Marcelo Rosales、Kaku Masaru、Nozaki Kosuke、Ida Takako、Kitami Megumi、Aoyagi Yujin、Uoshima Katsumi
  • Journal Title: Journal of Orthopaedic Surgery and Research Volume: 14 Issue: 1 Pages: 451-451
  • DOI: 10.1186/s13018-019-1485-2
  • Peer Reviewed / Open Access
• [Journal Article] オッセオインテグレーションの獲得に関わる骨代謝とコラーゲン架橋 (2019)
  • Author(s): 加来 賢、井田 貴子、長澤 麻沙子、魚島 勝美
  • Journal Title: 日本口腔インプラント学会誌 Volume: 32 Pages: 108-115
  • NAID: 130007689445
• [Journal Article] IFT20 is required for the maintenance of cartilaginous matrix in condylar cartilage (2019)
  • Author(s): Kitami Megumi、Yamaguchi Hiroyuki、Ebina Masayuki、Kaku Masaru、Chen Di、Komatsu Yoshihiro
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 509 Issue: 1 Pages: 222-226
  • DOI: 10.1016/j.bbrc.2018.12.107
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A multi-factorial analysis of bone morphology and fracture strength of rat femur in response to ovariectomy (2018)
  • Author(s): Rosales Rocabado Juan Marcelo、Kaku Masaru、Nozaki Kosuke、Ida Takako、Kitami Megumi、Aoyagi Yujin、Uoshima Katsumi
  • Journal Title: Journal of Orthopaedic Surgery and Research Volume: 13 Issue: 1 Pages: 318-328
  • DOI: 10.1186/s13018-018-1018-4
  • Peer Reviewed / Open Access
• [Journal Article] VWC2 Increases Bone Formation Through Inhibiting Activin Signaling (2018)
  • Author(s): Almehmadi Ahmad、Ohyama Yoshio、Kaku Masaru、Alamoudi Ahmed、Husein Dina、Katafuchi Michitsuna、Mishina Yuji、Mochida Yoshiyuki
  • Journal Title: Calcified Tissue International Volume: 103 Issue: 6 Pages: 663-674
  • DOI: 10.1007/s00223-018-0462-9
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Extracellular matrix with defective collagen cross-linking affects the differentiation of bone cells (2018)
  • Author(s): Ida Takako、Kaku Masaru、Kitami Megumi、Terajima Masahiko、Rosales Rocabado Juan Marcelo、Akiba Yosuke、Nagasawa Masako、Yamauchi Mitsuo、Uoshima Katsumi
  • Journal Title: PLOS ONE Volume: 13 Issue: 9 Pages: 1-18
  • DOI: 10.1371/journal.pone.0204306
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] BRCA1 and BRCA2 tumor suppressors in neural crest cells are essential for craniofacial bone development (2018)
  • Author(s): Kitami Kohei、Kitami Megumi、Kaku Masaru、Wang Bin、Komatsu Yoshihiro
  • Journal Title: PLOS Genetics Volume: 14 Issue: 5 Pages: e1007340-e1007340
  • DOI: 10.1371/journal.pgen.1007340
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] An ENU-induced splice site mutation of mouse Col1a1 causing recessive osteogenesis imperfecta and revealing a novel splicing rescue. (2017)
  • Author(s): Tabeta K, Du X, Arimatsu K, Yokoji M, Takahashi N, Amizuka N, Hasegawa T, Crozat K, Maekawa T, Miyauchi S, Matsuda Y, Ida T, Kaku M, Hoebe K, Ohno K, Yoshie H, Yamazaki K, Moresco EMY, Beutler B
  • Journal Title: Sci. Rep. Volume: 7(1) Issue: 1 Pages: 11717-11717
  • DOI: 10.1038/s41598-017-10343-9
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Functional Diversity of Ciliary Proteins in Bone Development and Disease (2017)
  • Author(s): Kaku M, Komatsu Y
  • Journal Title: Curr Osteoporos Rep Volume: Epub ahead of print Issue: 2 Pages: 96-102
  • DOI: 10.1007/s11914-017-0351-6
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] A paradigm shift for bone quality in dentistry: A literature review (2017)
  • Author(s): Kuroshima Shinichiro, Kaku Masaru, Ishimoto Takuya Sasaki Muneteru, Nakano Takayoshi, Sawase Takashi
  • Journal Title: Journal of Prosthodontic Research Volume: 61 Issue: 4 Pages: 353-362
  • DOI: 10.1016/j.jpor.2017.05.006
  • NAID: 130006198474
  • ISSN: 1883-1958, 1883-9207
  • Peer Reviewed / Open Access
• [Presentation] Fate of Tissue Stem Cells in Periodontal ligament. (2020)
  • Author(s): Kaku M
  • Organizer: Internatinal Colaborative Symposium on Development of Human Resources in Practical Oral Helath and Treatment
  • Int'l Joint Research / Invited
• [Presentation] Long-term lineage tracing of the periodontal ligament cells in vivo. (2020)
  • Author(s): Kaku M, Mizukoshi M, Kitami K, Rocabado JMR, Kaku S, Maeda T, Saito I, Uoshima K
  • Organizer: IADR General Session
  • Int'l Joint Research
• [Presentation] In Vivo Analysis of Cell Proliferation Dynamics in Periodontal Ligament. (2019)
  • Author(s): Kaku M, Mizukoshi M, Ida T, Kitami K, Uoshima K.
  • Organizer: ASMB 2019 Workshop Fibroblasts
  • Int'l Joint Research