Overexpression of DDX27 in gastric cancer(Fostering Joint International Research)

• Project/Area Number: 15KK0351
• Research Category: Fund for the Promotion of Joint International Research (Fostering Joint International Research)
• Allocation Type: Multi-year Fund
• Research Field: Experimental pathology
• Research Institution: Oita University
• Principal Investigator: TSUKAMOTO Yoshiyuki 大分大学, 医学部, 助教 (00433053)
• Research Collaborator: CLEVERS Hans ヒュブレヒト研究所, クレバースグループ, グループリーダー
• Project Period (FY): 2016 – 2017
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
• Keywords: オルガノイド / コラーゲン / 腸管幹細胞 / 癌 / 器官培養 / 遺伝子異常 / 幹細胞

Outline of Final Research Achievements

Principal investigator collaborated with Clevers Group (Hubrecht Institute, the Netherlands), where a three dimensional culture method for intestinal organoids has been established in 2009 (Sato et al, Nature 2009). In this study, we established macroscopic intestinal tubes from small cystic organoids by using floating collagen gels. The organoids cultured in soft-floating collagen fused together via collagen gel contraction and formed continuous tube structure. We found that the tubes contain stem and most types of the differentiated intestinal cells. In future, the macroscopic tubes might be used as an in vitro model of intestinal epithelium in investigations, such as drug effects and regenerative medicine.

Research Products

• [Int'l Joint Research] Hubrecht Institute(オランダ) (2017)
  • Year and Date: 2017-04-01
• [Journal Article] Oncogene-inducible organoids as a miniature platform to assess cancer characteristics (2017)
  • Author(s): Mizutani Tomohiro、Tsukamoto Yoshiyuki、Clevers Hans
  • Journal Title: J Cell Biol. Volume: 216 Issue: 6 Pages: 1505-1507
  • DOI: 10.1083/jcb.201704014
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Intestinal epithelial organoids fuse to form self-organizing tubes in floating collagen gels. (2017)
  • Author(s): Sachs N, Tsukamoto Y, Kujala P, Peters PJ, Clevers H.
  • Journal Title: Development Volume: 144(6) Issue: 6 Pages: 1107-1112
  • DOI: 10.1242/dev.143933
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Reduced phosphorylation of ribosomal protein S6 is associated with sensitivity to MEK inhibition in gastric cancer cells. (2016)
  • Author(s): Hirashita Y, Kodama M, Okimoto T, Murakami K, Moriyama M, et al.
  • Journal Title: Cancer Science Volume: 107(12) Issue: 12 Pages: 1919-1928
  • DOI: 10.1111/cas.13094
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Genomic loss of DUSP4 contributes to the progression of intraepithelial neoplasm of pancreas to invasive carcinoma. (2016)
  • Author(s): Hijiya N, Tsukamoto Y, Nakada C, Tung NL, Kai T, Matsuura K, Shibata K, Inomata M, Uchida T, Tokunaga A, Amada K, Shirao K, Yamada Y, Mori H, Takeuchi I, Seto M, Aoki M, Takekawa M, Moriyama M.
  • Journal Title: Cancer Res. Volume: 76 Issue: 9 Pages: 2612-2625
  • DOI: 10.1158/0008-5472.can-15-1846
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Kidney-specific knockout of Sav1 in the mouse promotes hyper-proliferation of renal tubular epithelium through suppression of the Hippo pathway. (2016)
  • Author(s): Kai T, Tsukamoto Y, Hijiya N, Tokunaga A, Nakada C, Uchida T, Daa T, Iha H, Takahashi M, Nomura T, Sato F, Mimata H, Ikawa M, Seto M, Matsuura K, Moriyama M.
  • Journal Title: J Pathol. Volume: 印刷中 Issue: 1 Pages: 97-108
  • DOI: 10.1002/path.4706
  • Peer Reviewed / Open Access
• [Journal Article] Downregulation of WDR20 due to loss of 14q is involved in the malignant transformation of clear cell renal cell carcinoma. (2016)
  • Author(s): Takahashi M, Tsukamoto Y, Kai T, Tokunaga A, Nakada C, Hijiya N, Uchida T, Daa T, Nomura T, Sato F, Mimata H, Matsuura K, Moriyama M.
  • Journal Title: Cancer Science Volume: 107(4) Issue: 4 Pages: 417-423
  • DOI: 10.1111/cas.12892
  • Peer Reviewed / Open Access