Identification and characterization of microbial drivers involved in nitrous oxide gas emission from upland field

• Project/Area Number: 15KT0024
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Multi-year Fund
• Section: 特設分野
• Research Field: Food Cycle Research
• Research Institution: The University of Tokyo
• Principal Investigator: Senoo Keishi 東京大学, 大学院農学生命科学研究科(農学部), 教授 (40206652)
• Co-Investigator(Kenkyū-buntansha): 磯部 一夫 東京大学, 大学院農学生命科学研究科(農学部), 助教 (30621833)
• Co-Investigator(Renkei-kenkyūsha): OTSUKA Shigeto 東京大学, 農学生命科学研究科, 准教授 (10313074)
• Research Collaborator: SHIRATORI Yutaka 新潟県農業総合研究所, 基盤研究部, 専門研究員 ; KAKUTA Hiroko 東京大学, 農学生命科学研究科 ; MASUDA Yoko 東京大学, 農学生命科学研究科 ; MASAKI Katsuya 東京大学, 農学生命科学研究科
• Project Period (FY): 2015-07-10 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000); Fiscal Year 2017: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2016: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2015: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
• Keywords: 一酸化二窒素ガス / 畑土壌 / 土壌微生物 / 環境調和型農業 / 土壌学 / N2Oガス発生 / 脱窒細菌 / N2O発生削減 / 植物生育促進効果 / ゲノム解読

Outline of Final Research Achievements

Large emission of nitrous oxide (N2O) gas is observed in upland field soil due to application of nitrogen fertilizer. To mitigate the N2O emission, identification and characterization of the microbial drivers of N2O generation and elimination in soils are essential. In this study, we successfully identify N2O generating/eliminating microorganisms in soil based on the analysis of soil DNA/RNA and bacterial isolates. N2O eliminating activity of large variety of bacterial isolates was enhanced by addition of Cu to culture medium. Based on the genome information, mechanism of plant growth promoting activity of two strains of N2O eliminating bacteria was clarified. These results will be useful to develop novel bio-fertilizer with both N2O mitigation and plant growth promoting ability.

Research Products

• [Journal Article] Nitrous oxide (N2O)-reducing denitrifier-inoculated organic fertilizer mitigates N2O emission from agricultural soils (2017)
  • Author(s): Nan Gao, Weishou Shen, Estefania Camargo, Yutaka Shiratori, Tomoyasu Nishizawa, Kazuo Isobe, Xinhua He, Keishi Senoo
  • Journal Title: Biology and Fertility of Soils Volume: 53 Issue: 8 Pages: 885-898
  • DOI: 10.1007/s00374-017-1231-z
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Azoarcus sp. KH32C株が保有するポリケチド合成酵素遺伝子の分子遺伝学的解析 (2018)
  • Author(s): 西澤 智康・迫田 翠・花岡 彩乃・鈴木 義人・山下 竜司・兼井 啓介・原田 健一・妹尾 啓史
  • Organizer: 日本ゲノム微生物学会年会
• [Presentation] Microbial Drivers of Nitrogen Transformation in Rice Paddy Soil: Identification, Isolation and Application to N2O Mitigation (2018)
  • Author(s): Keishi Senoo
  • Organizer: 南京農業大学セミナー
  • Int'l Joint Research / Invited
• [Presentation] Microbial Drivers of Nitrogen Transformation in Rice Paddy Soil: Identification, Isolation and Application to N2O Mitigation (2018)
  • Author(s): Keishi Senoo
  • Organizer: 南京信息工程大学セミナー
  • Int'l Joint Research / Invited
• [Presentation] Identification and isolation of active N2O reducers in rice paddy soil, and application to N2O mitigation (2018)
  • Author(s): Keishi Senoo
  • Organizer: China-Japan Symposium on Rhizosphere Cross-talk (NFSC-JST Cooperative Research Projects), Nanjing
  • Int'l Joint Research / Invited
• [Presentation] 水田土壌の窒素変換微生物：特定・分離とN2O削減への利用 (2017)
  • Author(s): 妹尾啓史
  • Organizer: 第34回 土・水研究会
  • Invited
• [Presentation] Microbial Drivers of Nitrogen Transformation in Rice Paddy Soil: Identification, Isolation and Application to N2O Mitigation (2017)
  • Author(s): Keishi Senoo
  • Organizer: 浙江大学セミナー
  • Int'l Joint Research / Invited
• [Presentation] Who produces N2O in cropland fields? (2015)
  • Author(s): Isobe, Kazuo, Wei, Wei, Shiratori, Yutaka, Nishizawa, Tomoyasu, Otsuka, Shigeto, Senoo, Keishi
  • Organizer: 日本微生物生態学会第30回大会、7th JTK Symposium
  • Place of Presentation: 土浦
  • Year and Date: 2015-09-17
  • Int'l Joint Research
• [Presentation] 陸域環境における亜硝酸還元酵素遺伝子の分布と組成 (2015)
  • Author(s): 畔上 洋一, 磯部 一夫, 魏 魏, 角田 洋子, 白鳥 豊, 西澤 智康, 木庭啓介 啓介, 大手 信人, 大塚 重人, 妹尾 啓史
  • Organizer: 日本微生物生態学会第30回大会、7th JTK Symposium
  • Place of Presentation: 土浦
  • Year and Date: 2015-09-17
  • Int'l Joint Research
• [Patent(Industrial Property Rights)] 農耕地用一酸化二窒素低減材 (2017)
  • Inventor(s): 白鳥豊、土田沙由里、妹尾啓史、平柳貴弘、永峰賢、深野透
  • Industrial Property Rights Holder: 白鳥豊、土田沙由里、妹尾啓史、平柳貴弘、永峰賢、深野透
  • Industrial Property Rights Type: 特許
  • Filing Date: 2017