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Mathematical prediction/analysis method on variation of tea-secondary metabolites in the course of cultivation and processing

Research Project

Project/Area Number 15KT0030
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeMulti-year Fund
Section特設分野
Research Field Food Cycle Research
Research InstitutionShizuoka University

Principal Investigator

WATANABE Naoharu  静岡大学, 創造科学技術大学院, 特任教授 (90230979)

Co-Investigator(Kenkyū-buntansha) 吉村 仁  静岡大学, 創造科学技術大学院, 教授 (10291957)
大西 利幸  静岡大学, 農学部, 准教授 (60542165)
鈴木 秀幸  公益財団法人かずさDNA研究所, バイオ研究開発部, グループ長 (80276162)
Co-Investigator(Renkei-kenkyūsha) SETOU Mitsutoshi  浜松医科大学, 医学部, 教授 (20302664)
MASE Nobuyuki  静岡大学, 工学部, 教授 (40313936)
Research Collaborator SATO Kohei  静岡大学, 工学部, 助教 (30756705)
KATSUNO Tsuyoshi  静岡県農業技術研究所, 茶業研究センター, 上席研究員 (50463188)
Project Period (FY) 2015-07-10 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2017: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2016: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2015: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Keywordsチャ栽培 / 茶加工 / プロトプラスト / アポプラスト / 配糖体合成 / DNA-シークエンシング / LC-MS/MS / ウンカ / チャ葉 / 数理解析モデル / RNA-Seq / チャ / 数理解析 / コンパートメントモデル / 香気成分配糖体 / 次世代シークエンス / 液胞
Outline of Final Research Achievements

Tea-volatile compounds and related secondary metabolites together with the glycoconjugates of alcoholic aroma compounds : GBVs were analyzed during cultivation at different environmental conditions (season, insects attack) and process of manufacturing (temperature, time). Based on the analytical data we have designed mathematical model for prediction of optimal conditions for tea processing. We have clarified that increase in GBVs is ascribed to enzymatic synthesis during the withering process of tea leaves. During the low temperature-withering process, the maximum levels were detected in 10-16 hr during the process, suggesting that temperature and period of withering process are the crucial factors to increase the tea aroma. Volatile constituents were varied by plucking seasons and presence or absence of damages by insects-attacks.

Report

(4 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (27 results)

All 2018 2017 2016 2015 Other

All Int'l Joint Research (3 results) Journal Article (16 results) (of which Int'l Joint Research: 13 results,  Peer Reviewed: 16 results,  Open Access: 16 results,  Acknowledgement Compliant: 1 results) Presentation (7 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results) Book (1 results)

  • [Int'l Joint Research] 中国科学院華南植物園(中国)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] 華南植物園(中国)

    • Related Report
      2016 Research-status Report
  • [Int'l Joint Research] 中国科学院華南植物園/西南大学(中国)

    • Related Report
      2015 Research-status Report
  • [Journal Article] Tuning the catalyst reactivity of imidazolylidene catalysts through substituent effects on the N-aryl groups2017

    • Author(s)
      R. Kyan, K. Sato, N. Mase, N. Watanabe, T. Narumi
    • Journal Title

      Org. Lett.

      Volume: 19 Issue: 10 Pages: 2750-2753

    • DOI

      10.1021/acs.orglett.7b01105

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Fine-bubble-based strategy for the palladium-catalyzed hydrogenation of nitro groups: measurement of ultrafine bubbles in organic solvents2017

    • Author(s)
      N. Mase, Y. Nishina, S. Isomura, K. Sato, T. Narumi, N. Watanabe
    • Journal Title

      Synlett

      Volume: 28 Issue: 16 Pages: 2184-2188

    • DOI

      10.1055/s-0036-1588869

    • NAID

      120006488774

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Insects (Thrips hawaiiensis (Morgan)) change the stereochemical configuration of 1-phenylethanol emitted from tea (Camellia sinensis) flowers2017

    • Author(s)
      Zhou,Y.,Zeng,L., Liao,Y., Dong,F., Peng,Q., Li,J., Tang, J., Watanabe, N., Yang, Z.
    • Journal Title

      RSC Advances

      Volume: 7 Pages: 32336-32343

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Analytical method for metabolites involved in biosynthesis of plant volatile compounds2017

    • Author(s)
      Fu,X., Zhou, Y., Zeng,L., Dong,F., Mei,X., Liao,Y., Watanabe,N., Yang,Z.
    • Journal Title

      RSC Advances

      Volume: 7 Issue: 31 Pages: 19363-19372

    • DOI

      10.1039/c7ra00766c

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Optimization of the production of 1-phenylethanol using enzymes from flowers of tea (Camellia sinensis) plants2017

    • Author(s)
      Dong, F., Zhou, Y., Zeng, L., Watanabe, N., Su, X., Yang, Z
    • Journal Title

      Molecules

      Volume: 22 Pages: 131-131

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Analytical method for metabolites involed in biosynthesis of plant volatile compounds2017

    • Author(s)
      Fu, X., Zhou, Y., Zeng, L., Dong, F., Mei, X., Liao,,Y.,Watanabe, N., Yang. Z
    • Journal Title

      RSC Advances

      Volume: 31 Pages: 19363-19372

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Formation of characteristic aroma compound indole during the oolong tea manufacturing process2016

    • Author(s)
      Zeng, L, Zhou, Y, Gui, J, Fu, X, Mei, X, Zhen, Y, Ye, T, Du, B, Dong, F, Watanabe, N, Yang. Z
    • Journal Title

      J. Agric Food Chem

      Volume: 21 Pages: 5011-5019

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Elucidation of differential accumulation of 1-phenylethanol in flowers and leaves of tea (Camellia sinensis) plants2016

    • Author(s)
      Dong, F., Shou, Y., Peng, Q., Chen, Y., Zhang, L., Su, X., Watanabe, N., Yang, Z.
    • Journal Title

      Molecules

      Volume: 21 Issue: 9 Pages: 1106-1106

    • DOI

      10.3390/molecules21091106

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Synthesis of a self-assembling gold nanoparticle-supported organocatalyst for enamine-based asymmetric aldol reactions.2016

    • Author(s)
      Soti, P.Z., Yamashita,H., Sato,K., Narumi, T., Toda, M., Watanabe, N., Marosi,G., Mase, N.
    • Journal Title

      Tetrahedron

      Volume: 72 Issue: 16 Pages: 1984-1990

    • DOI

      10.1016/j.tet.2016.02.065

    • NAID

      120006411284

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Recent advances in the emission and functions of plant vegetative volatiles2016

    • Author(s)
      Dong, F., Fu, X., Watanabe, N., Su, X., Yang
    • Journal Title

      Molecules

      Volume: 21 Issue: 2 Pages: 124-124

    • DOI

      10.3390/molecules21020124

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Characteristic fluctuations in glycosidically bound volatiles during tea processing and identification of their unstable derivatives2016

    • Author(s)
      Cui, J., Katsuno, T., Totsuka, K., Ohnishi,T., Takemoto, H., Mase,N., Toda, M.,Narumi, T.,Sato, K., Matsuo, T., Mizutani, K., Yang, Z., Watanabe, N., Tong, H.
    • Journal Title

      J. Agric. Food Chem.

      Volume: 64 Issue: 5 Pages: 1151-1157

    • DOI

      10.1021/acs.jafc.5b05072

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Seasonal induction of alternative principal pathway for rose flower scent.2016

    • Author(s)
      Hirata, H., Ohnishi, T., Tomida, K., Ishida, H., Kanda, M., Sakai, M., Yoshimura, J., Suzuki, H., Ishikawa, T., Dohra, H., Watanabe, N
    • Journal Title

      Sci. Rep.

      Volume: 6 Issue: 1 Pages: 1-9

    • DOI

      10.1038/srep20234

    • NAID

      120005771327

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Regulation of formation of volatile compounds of preharvest and postharvest tea (Camellia sinensis) leaves by single light wavelength2015

    • Author(s)
      Fu, X., Chen,Y., Mei, X., Katsuno,T., Kobayashi, E., Dong, F., Watanabe, N., Yang, Z.
    • Journal Title

      Sci. Rep.

      Volume: 5 Issue: 1

    • DOI

      10.1038/srep16858

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Does enzymatic hydrolysis of glycosidically bound volatile compounds really contribute to the formation of volatile compounds during the oolong tea manufacturing process?2015

    • Author(s)
      Gui, J.; Fu, X.; Zhou, Y.; Katsuno, T.; Mei, X.; Deng, R.; Xu, X.; Zhang, L.; Dong, F.; Watanabe, N.; Yang, Z
    • Journal Title

      J. Agric. Food Chem.

      Volume: 63 Issue: 31 Pages: 6905-6914

    • DOI

      10.1021/acs.jafc.5b02741

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Position-specific isotope analysis of xanthines: a 13C Nuclear magnetic resonance method to determine the 13C intramolecular composition at natural abundance.2015

    • Author(s)
      Diomande, D., Martineau,E., Gilbert, A., Nun, P., Murata, A., Yamada, K., Watanabe, N., Tea, I., Robins, R., Yoshida, N., Remaud, G.
    • Journal Title

      Anal. Chem.

      Volume: 87 Pages: 6600-6606

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Volatile glycosylation in tea plants: Sequential glycosilations for the biosynthesis of aroma b-primeverosides are catalyzed by two Camellia sinensis glycosyltransferases.2015

    • Author(s)
      Ohgami, S., Ono, E., Horikawa, M., Murata, M., Totsuka, K., Toyonaga, H., Ohba, Y., Dohra, H., Asai, T., Matsui, K., Mizutani, M., Watanabe, N., Ohnishi, T.
    • Journal Title

      Plant Physiol.

      Volume: 168 Issue: 2 Pages: 464-477

    • DOI

      10.1104/pp.15.00403

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] NMR,MSで探る花の不思議2018

    • Author(s)
      渡辺修治
    • Organizer
      静岡大学共同利用機器センター主催基礎分析講座
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Research and development on tea sciences and technologies in Shizuoka, Japan2017

    • Author(s)
      Naoharu Watanabe
    • Organizer
      Tea EXPO 2017, Hangzhou, China
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 花芽誘導物質LDS1 構造要求性の解明2016

    • Author(s)
      山田 優作、土屋 諒、廣住 操俊、佐藤 浩平、鳴海 哲夫、戸田 三津夫、大西 利幸、間瀬 暢之、渡辺 修治
    • Organizer
      日本農芸化学会2016年度大会
    • Place of Presentation
      札幌コンベンションセンター(札幌市)
    • Year and Date
      2016-03-30
    • Related Report
      2015 Research-status Report
  • [Presentation] 7-ヒドロキシキノリニウム型ケージド基の創製研究:キノリンのN-アルキル化による高度化、2016

    • Author(s)
      二位 明崇, 山口 竣, 佐藤 浩平, 間瀬 暢之, 渡辺 修治, 鳴海 哲夫
    • Organizer
      日本薬学会第136回年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-03-28
    • Related Report
      2015 Research-status Report
  • [Presentation] 化学選択的誘導体化によるイオン強度増大を指向したイメージング質量分析におけるラベル化剤の開発2016

    • Author(s)
      岡村 拓磨・瀬藤 光利・佐藤 浩平・鳴海 哲夫・渡辺 修治・間瀬 暢之
    • Organizer
      日本化学会第96春季年会
    • Place of Presentation
      同志社大学(京都府京都市)
    • Year and Date
      2016-03-25
    • Related Report
      2015 Research-status Report
  • [Presentation] チャ香気に関与するテルペン生合成酵素の機能解明2015

    • Author(s)
      大西利幸, 井上智宏, 渡辺修治, 鈴木秀幸
    • Organizer
      植物化学調節学会第50回大会
    • Place of Presentation
      東京大学農学部(東京都文京区)
    • Year and Date
      2015-10-01
    • Related Report
      2015 Research-status Report
  • [Presentation] 花芽誘導物質LDS1および構造改変体の合成2015

    • Author(s)
      山田優作、土屋諒、廣住操俊、佐藤浩平、鳴海哲夫、戸田三津夫、大西利幸、間瀬暢之、渡辺修治
    • Organizer
      2015年度日本農芸化学会中部・関西支部合同大会(中部支部第147回例会・関西支部第471回講演会)
    • Place of Presentation
      富山県立大学(富山県射水市)
    • Year and Date
      2015-09-20
    • Related Report
      2015 Research-status Report
  • [Book] ナノ・バイオテクノロジー2016

    • Author(s)
      原正和, 渡辺修治, 間瀬暢之, 河岸洋和, 森田達也, 丑丸敬史, 朴龍洙, 猪川洋, 岩田太, 川田善正, 三重野哲, 加藤竜也, 徳元俊伸, 山崎昌一, 小林健二, 山中正道
    • Total Pages
      268
    • Publisher
      静岡学術出版
    • Related Report
      2015 Research-status Report

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Published: 2015-07-14   Modified: 2019-03-29  

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