Mathematical prediction/analysis method on variation of tea-secondary metabolites in the course of cultivation and processing
Project/Area Number |
15KT0030
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Multi-year Fund |
Section | 特設分野 |
Research Field |
Food Cycle Research
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Research Institution | Shizuoka University |
Principal Investigator |
WATANABE Naoharu 静岡大学, 創造科学技術大学院, 特任教授 (90230979)
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Co-Investigator(Kenkyū-buntansha) |
吉村 仁 静岡大学, 創造科学技術大学院, 教授 (10291957)
大西 利幸 静岡大学, 農学部, 准教授 (60542165)
鈴木 秀幸 公益財団法人かずさDNA研究所, バイオ研究開発部, グループ長 (80276162)
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Co-Investigator(Renkei-kenkyūsha) |
SETOU Mitsutoshi 浜松医科大学, 医学部, 教授 (20302664)
MASE Nobuyuki 静岡大学, 工学部, 教授 (40313936)
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Research Collaborator |
SATO Kohei 静岡大学, 工学部, 助教 (30756705)
KATSUNO Tsuyoshi 静岡県農業技術研究所, 茶業研究センター, 上席研究員 (50463188)
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Project Period (FY) |
2015-07-10 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2017: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2016: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2015: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
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Keywords | チャ栽培 / 茶加工 / プロトプラスト / アポプラスト / 配糖体合成 / DNA-シークエンシング / LC-MS/MS / ウンカ / チャ葉 / 数理解析モデル / RNA-Seq / チャ / 数理解析 / コンパートメントモデル / 香気成分配糖体 / 次世代シークエンス / 液胞 |
Outline of Final Research Achievements |
Tea-volatile compounds and related secondary metabolites together with the glycoconjugates of alcoholic aroma compounds : GBVs were analyzed during cultivation at different environmental conditions (season, insects attack) and process of manufacturing (temperature, time). Based on the analytical data we have designed mathematical model for prediction of optimal conditions for tea processing. We have clarified that increase in GBVs is ascribed to enzymatic synthesis during the withering process of tea leaves. During the low temperature-withering process, the maximum levels were detected in 10-16 hr during the process, suggesting that temperature and period of withering process are the crucial factors to increase the tea aroma. Volatile constituents were varied by plucking seasons and presence or absence of damages by insects-attacks.
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Report
(4 results)
Research Products
(27 results)
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[Journal Article] Insects (Thrips hawaiiensis (Morgan)) change the stereochemical configuration of 1-phenylethanol emitted from tea (Camellia sinensis) flowers2017
Author(s)
Zhou,Y.,Zeng,L., Liao,Y., Dong,F., Peng,Q., Li,J., Tang, J., Watanabe, N., Yang, Z.
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Journal Title
RSC Advances
Volume: 7
Pages: 32336-32343
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Analytical method for metabolites involved in biosynthesis of plant volatile compounds2017
Author(s)
Fu,X., Zhou, Y., Zeng,L., Dong,F., Mei,X., Liao,Y., Watanabe,N., Yang,Z.
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Journal Title
RSC Advances
Volume: 7
Issue: 31
Pages: 19363-19372
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Optimization of the production of 1-phenylethanol using enzymes from flowers of tea (Camellia sinensis) plants2017
Author(s)
Dong, F., Zhou, Y., Zeng, L., Watanabe, N., Su, X., Yang, Z
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Journal Title
Molecules
Volume: 22
Pages: 131-131
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Analytical method for metabolites involed in biosynthesis of plant volatile compounds2017
Author(s)
Fu, X., Zhou, Y., Zeng, L., Dong, F., Mei, X., Liao,,Y.,Watanabe, N., Yang. Z
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Journal Title
RSC Advances
Volume: 31
Pages: 19363-19372
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Formation of characteristic aroma compound indole during the oolong tea manufacturing process2016
Author(s)
Zeng, L, Zhou, Y, Gui, J, Fu, X, Mei, X, Zhen, Y, Ye, T, Du, B, Dong, F, Watanabe, N, Yang. Z
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Journal Title
J. Agric Food Chem
Volume: 21
Pages: 5011-5019
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Elucidation of differential accumulation of 1-phenylethanol in flowers and leaves of tea (Camellia sinensis) plants2016
Author(s)
Dong, F., Shou, Y., Peng, Q., Chen, Y., Zhang, L., Su, X., Watanabe, N., Yang, Z.
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Journal Title
Molecules
Volume: 21
Issue: 9
Pages: 1106-1106
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Synthesis of a self-assembling gold nanoparticle-supported organocatalyst for enamine-based asymmetric aldol reactions.2016
Author(s)
Soti, P.Z., Yamashita,H., Sato,K., Narumi, T., Toda, M., Watanabe, N., Marosi,G., Mase, N.
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Journal Title
Tetrahedron
Volume: 72
Issue: 16
Pages: 1984-1990
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Characteristic fluctuations in glycosidically bound volatiles during tea processing and identification of their unstable derivatives2016
Author(s)
Cui, J., Katsuno, T., Totsuka, K., Ohnishi,T., Takemoto, H., Mase,N., Toda, M.,Narumi, T.,Sato, K., Matsuo, T., Mizutani, K., Yang, Z., Watanabe, N., Tong, H.
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Journal Title
J. Agric. Food Chem.
Volume: 64
Issue: 5
Pages: 1151-1157
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Seasonal induction of alternative principal pathway for rose flower scent.2016
Author(s)
Hirata, H., Ohnishi, T., Tomida, K., Ishida, H., Kanda, M., Sakai, M., Yoshimura, J., Suzuki, H., Ishikawa, T., Dohra, H., Watanabe, N
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Journal Title
Sci. Rep.
Volume: 6
Issue: 1
Pages: 1-9
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Regulation of formation of volatile compounds of preharvest and postharvest tea (Camellia sinensis) leaves by single light wavelength2015
Author(s)
Fu, X., Chen,Y., Mei, X., Katsuno,T., Kobayashi, E., Dong, F., Watanabe, N., Yang, Z.
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Journal Title
Sci. Rep.
Volume: 5
Issue: 1
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Does enzymatic hydrolysis of glycosidically bound volatile compounds really contribute to the formation of volatile compounds during the oolong tea manufacturing process?2015
Author(s)
Gui, J.; Fu, X.; Zhou, Y.; Katsuno, T.; Mei, X.; Deng, R.; Xu, X.; Zhang, L.; Dong, F.; Watanabe, N.; Yang, Z
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Journal Title
J. Agric. Food Chem.
Volume: 63
Issue: 31
Pages: 6905-6914
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Position-specific isotope analysis of xanthines: a 13C Nuclear magnetic resonance method to determine the 13C intramolecular composition at natural abundance.2015
Author(s)
Diomande, D., Martineau,E., Gilbert, A., Nun, P., Murata, A., Yamada, K., Watanabe, N., Tea, I., Robins, R., Yoshida, N., Remaud, G.
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Journal Title
Anal. Chem.
Volume: 87
Pages: 6600-6606
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Volatile glycosylation in tea plants: Sequential glycosilations for the biosynthesis of aroma b-primeverosides are catalyzed by two Camellia sinensis glycosyltransferases.2015
Author(s)
Ohgami, S., Ono, E., Horikawa, M., Murata, M., Totsuka, K., Toyonaga, H., Ohba, Y., Dohra, H., Asai, T., Matsui, K., Mizutani, M., Watanabe, N., Ohnishi, T.
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Journal Title
Plant Physiol.
Volume: 168
Issue: 2
Pages: 464-477
DOI
Related Report
Peer Reviewed / Open Access
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[Book] ナノ・バイオテクノロジー2016
Author(s)
原正和, 渡辺修治, 間瀬暢之, 河岸洋和, 森田達也, 丑丸敬史, 朴龍洙, 猪川洋, 岩田太, 川田善正, 三重野哲, 加藤竜也, 徳元俊伸, 山崎昌一, 小林健二, 山中正道
Total Pages
268
Publisher
静岡学術出版
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