Development of nucleic acid enzymes for new nucleic acid medicines based on the transition state in molecular degradation
| Project/Area Number |
15KT0057
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Multi-year Fund |
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| Section | 特設分野 |
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| Research Field |
Transition State Control
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Okamoto Akimitsu 東京大学, 先端科学技術研究センター, 教授 (60314233)
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| Co-Investigator(Kenkyū-buntansha) |
林 剛介 東京大学, 大学院工学系研究科(工学部), 助教 (40648268)
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| Project Period (FY) |
2015-07-10 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2017: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2016: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2015: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
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| Keywords | RNA / アプタマー / 核酸構造 / バクテリア / 核酸 / 蛍光 / 発酵 / 遷移状態 / ヒュースゲン環化 |
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| Outline of Final Research Achievements |
The solid-state fermentation (SSF) bioprocesses are performed in complex solid-rich systems that present significant challenges for effective monitoring of bacterial population dynamics. We have developed an efficient chemical system that allows quantification of bacteria population by fluorescence-based analysis. The key component in the system is the exciton-controlled fluorescent RNA aptamer, which was covalently conjugated to two thiazole orange moieties and serves as a competitor of bacterial ribosome. The intensity of fluorescence from such a ribosome-sensing system was controlled by the excitonic interaction between dyes in the RNA aptamer and it increased drastically in the presence of Escherichia coli. This innovative fluorescence-based competition system is valuable for quantification without any extraction of bacterial nucleic acids, and provides the simplest and most feasible way to optimize SSF bioprocesses.
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Report
(4 results)
Research Products
(2 results)
