| Budget Amount *help |
¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
Fiscal Year 2017: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2016: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2015: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
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| Outline of Final Research Achievements |
The solid-state fermentation (SSF) bioprocesses are performed in complex solid-rich systems that present significant challenges for effective monitoring of bacterial population dynamics. We have developed an efficient chemical system that allows quantification of bacteria population by fluorescence-based analysis. The key component in the system is the exciton-controlled fluorescent RNA aptamer, which was covalently conjugated to two thiazole orange moieties and serves as a competitor of bacterial ribosome. The intensity of fluorescence from such a ribosome-sensing system was controlled by the excitonic interaction between dyes in the RNA aptamer and it increased drastically in the presence of Escherichia coli. This innovative fluorescence-based competition system is valuable for quantification without any extraction of bacterial nucleic acids, and provides the simplest and most feasible way to optimize SSF bioprocesses.
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