| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2018: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2017: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2015: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
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| Outline of Final Research Achievements |
The adaptation phenomena of bacterial clonal cell populations include persistence, which occurs on the timescale of several generations, and long-term adaptation that occurs over tens to hundreds of generations. In this research project, we constructed E. coli cell strains with altered statistical properties of expression noise of survival-correlated genes. We then analyzed their drug response in detail using our unique single-cell measurement technology. We also constructed an analytical framework for quantitative evaluation of fitness landscape and selection strength based on the information of cell lineage trees and expression fluctuation. From these experiments, we revealed that multiple survival modes coexist in bacterial persistence and that long-term adaptation to drugs occurs through the remodeling of global gene expression state.
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