| Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2017: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2016: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2015: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
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| Outline of Final Research Achievements |
Using Escherichia coli, a model bacterium, an evolution experiment was performed under highly mutagenic environment for several years. Analyzing genomic mutations revealed that mutations tends were enriched in genes related to stress response. In addition, growth reduction under highly mutagenic environment was derived not only by deleterious load of the accumulated mutations, but also by another harmful effect associated with mutagenic physiology. Moreover, mutations were likely to be accumulated at a specific sequence motif which consists of few nucleotide at least. These evidence would help us identify specific genes responsible for growth robustness against deleterious mutations at the genomic scale and design mutationally stable genome.
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