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Experimental evolution of an primitive cell model to understand possible evolutionary process

Research Project

Project/Area Number 15KT0080
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeMulti-year Fund
Section特設分野
Research Field Constructive Systems Biology
Research InstitutionThe University of Tokyo (2018-2019)
Osaka University (2015-2017)

Principal Investigator

Ichihashi Norikazu  東京大学, 大学院総合文化研究科, 教授 (20448096)

Project Period (FY) 2015-07-10 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2015: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
KeywordsRNA / 進化 / 生命の起源 / 協力関係 / 共進化 / 実験進化 / 協力関係の進化 / 分子進化 / 自己複製RNA / 協力 / 複製 / NDK / ハイパーサイクル / 再構成 / RNA複製 / 生命の初期進化 / 翻訳システム
Outline of Final Research Achievements

In this study, we investigate the conditions that allow the maintenance and development of cooperation between primitive self-replicators. First, we construct two types of RNAs that encode different functions. Second, we performed a long-term evolutionary experiment of the RNAs and found that the most important conditions that allow the maintenance and development of the cooperation are micro-compartmentalization, the average RNA concentration, and the mixing rate of the compartments. The results were supported by computer simulation. This study revealed essential conditions under which primitive life-forms could develop their functions.

Academic Significance and Societal Importance of the Research Achievements

単純な化合物からどうやって生命が生まれたのかは、人類に課された大きな謎のひとつである。本研究では、原始の生命体の実験モデルをつくってみて、実際に試験管内で進化させてみることにより生命誕生の秘密を明らかにすることを試みた。生命がうまれるためには多くの機能を進化させる必要があり、そのためには異なる機能を持ったRNAが協力して働く必要があると言われている。本研究ではどうやったらRNA間の協力関係が安定になるのかを明らかにすることができた。本研究結果は、生命が誕生するための条件としてRNAの濃度や攪拌の程度が重要だと示した。この結果により、生命の誕生場の条件を絞り込むことができるだろう。

Report

(6 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • 2015 Research-status Report
  • Research Products

    (20 results)

All 2019 2018 2017 2016 2015

All Journal Article (10 results) (of which Peer Reviewed: 10 results,  Open Access: 7 results,  Acknowledgement Compliant: 1 results) Presentation (9 results) (of which Int'l Joint Research: 6 results,  Invited: 8 results) Book (1 results)

  • [Journal Article] A Fusion Method to Develop an Expanded Artificial Genomic RNA Replicable by Qβ Replicase2019

    • Author(s)
      Ueda Kensuke、Mizuuchi Ryo、Matsuda Fumio、Ichihashi Norikazu
    • Journal Title

      ChemBioChem

      Volume: 20 Issue: 18 Pages: 2331-2335

    • DOI

      10.1002/cbic.201900120

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural transition of replicable RNAs during in vitro evolution with Qβ replicase2019

    • Author(s)
      Mizuuchi Ryo、Usui Kimihito、Ichihashi Norikazu
    • Journal Title

      RNA

      Volume: 26 Issue: 1 Pages: 83-90

    • DOI

      10.1261/rna.073106.119

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Automated in vitro evolution of a translation-coupled RNA replication system in a droplet flow reactor2018

    • Author(s)
      Yoshiyama Tomoaki、Ichii Tetsuo、Yomo Tetsuya、Ichihashi Norikazu
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 11867-11875

    • DOI

      10.1038/s41598-018-30374-0

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Sustainable replication and coevolution of cooperative RNAs in an artificial cell-like system2018

    • Author(s)
      Mizuuchi Ryo、Ichihashi Norikazu
    • Journal Title

      Nature Ecology & Evolution

      Volume: 2 Issue: 10 Pages: 1654-1660

    • DOI

      10.1038/s41559-018-0650-z

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Sustainability of a compartmentalized host-parasite replicator system2018

    • Author(s)
      Furubayashi, T., Ichihashi, N.
    • Journal Title

      Life

      Volume: 8 Issue: 1 Pages: 1-10

    • DOI

      10.3390/life8010003

    • Related Report
      2018 Research-status Report 2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Combinatorial selection for replicable RNA by Qβ replicase while maintaining encoded gene function2017

    • Author(s)
      Yumura, M., Yamamoto, N., Yokoyama, K., Mori, H., Yomo, T., Ichihashi, N.
    • Journal Title

      Plos ONE

      Volume: 12 Issue: 3 Pages: 1-14

    • DOI

      10.1371/journal.pone.0174130

    • Related Report
      2017 Research-status Report 2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] A transcription and translation-coupled DNA replication system using rolling-circle replication2015

    • Author(s)
      Sakatani, Y., Ichihashi, N., Kazuta, Y., Yomo, T.
    • Journal Title

      Scientific Reports

      Volume: 5

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Periodic Pattern of Genetic and Fitness Diversity during Evolution of an Artificial Cell-Like System2015

    • Author(s)
      Ichihashi, N., Aita, T., Motooka, D., Nakamura, S., Yomo, T.
    • Journal Title

      Molecular Biology and Evolution

      Volume: 32 Pages: 3205-3214

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Replication of partial double-stranded RNAs by Qbeta replicase2015

    • Author(s)
      Tomita, K., Ichihashi, N., Yomo, T.
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 467 Pages: 293-296

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] A design principle for a single-stranded RNA genome that replicates with less double-strand formation2015

    • Author(s)
      Usui, K., Ichihashi, N., Yomo, T.
    • Journal Title

      Nucleic Acids Research

      Volume: 43 Pages: 8033-8043

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Making an artificial cell capable of Darwinian evolution2019

    • Author(s)
      Norikazu Ichihashi
    • Organizer
      BDR symposium
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Experimental evolution of self-replicating RNAs with spontaneously-appeared parasites2018

    • Author(s)
      Norikazu Ichihashi
    • Organizer
      46th Naito Conference
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Sustainability and evolvability of cooperative RNAs in an artificial cell-like system2018

    • Author(s)
      Norikazu Ichihashi
    • Organizer
      Biophysics Society Annual Meeting
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] Experimental evolution of self-replicating RNAs with spontaneously-appeared parasites2018

    • Author(s)
      Norikazu Ichihashi
    • Organizer
      EVOLUTION Genetic Novelty/Genomic Variations by RNA Networks and Viruses
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Evolution of a self-replicable RNA, the roles of compartment, parasites, and hypercycle2017

    • Author(s)
      Norikazu Ichihashi
    • Organizer
      International Conference The Origin of life
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Host-parasite oscillation dynamics and evolution in a compartmentalized RNA replication system2017

    • Author(s)
      Y. Bansho , T. Furubayashi , N. Ichihashi
    • Organizer
      XVIIIth International Conference on the Origin of Life
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 実験進化で探る 物質から生命への進化可能性2017

    • Author(s)
      市橋 伯一
    • Organizer
      生命の起源と進化学会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] Synthesizing an evolvable replication system from bacterial components2016

    • Author(s)
      Ichihashi N.
    • Organizer
      Synthetic biology workshop
    • Place of Presentation
      奈良先端科学技術大学院大学(奈良県・生駒市)
    • Year and Date
      2016-03-17
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 人工RNA複製システムの構築と寄生体との共進化2015

    • Author(s)
      市橋伯一
    • Organizer
      進化学会年会
    • Place of Presentation
      中央大学(東京都・文京区)
    • Year and Date
      2015-08-20
    • Related Report
      2015 Research-status Report
    • Invited
  • [Book] 協力と裏切りの生命進化史2019

    • Author(s)
      市橋伯一
    • Total Pages
      240
    • Publisher
      光文社
    • ISBN
      9784334044008
    • Related Report
      2018 Research-status Report

URL: 

Published: 2015-07-14   Modified: 2022-11-04  

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